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Prepublished online as a Blood First Edition Paper on May 29, 2003; DOI 10.1182/blood-2002-10-3190. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-10-3190v1 102/6/1978    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wright, N. Articles by Teixidó, J. Search for Related Content PubMed PubMed Citation Articles by Wright, N. Articles by Teixidó, J. Related Collections Hematopoiesis and Stem Cells Immunobiology Cell Adhesion and Motility Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 September 2003, Vol. 102, No. 6, pp. 1978-1984 CHEMOKINES Transforming growth factor-1 down-regulates expression of chemokine stromal cell–derived factor-1: functional consequences in cell migration and adhesion Natalia Wright, Teresa Laín de Lera, Carelia García-Moruja, Rosa Lillo, Félix García-Sánchez, Antonio Caruz, and Joaquin Teixidó From the Centro de Investigaciones Biológicas, Department of Immunology, Madrid, Spain; Department of Experimental Biology, University of Jaén, Spain; and Histocompatibility and Molecular Biology Laboratory, Centro de Transfusión de la Comunidad de Madrid, Spain. Chemokine stromal cell–derived factor-1 (SDF-1) is expressed by bone marrow (BM) stromal cells and plays key roles in BM cell migration. Modulation of its expression could affect the migratory capacity of cells trafficking the BM, such as hematopoietic progenitor and leukemic cells. Transforming growth factor-1 (TGF-1) is present in the BM environment and constitutes a pivotal molecule controlling BM cell proliferation and differentiation. We used the BM stromal cell line MS-5 as a model to investigate whether SDF-1 expression constitutes a target for TGF-1 regulation and its functional consequences. We show here that TGF-1 down-regulates SDF-1 expression, both at the mRNA level, involving a decrease in transcriptional efficiency, and at the protein level, as detected in lysates and supernatants from MS-5 cells. Reduction of SDF-1 in supernatants from TGF-1–treated MS-5 cells correlated with decreased, SDF-1–dependent, chemotactic, and transendothelial migratory responses of the BM model cell lines NCI-H929 and Mo7e compared with their responses to supernatants from untreated MS-5 cells. In addition, supernatants from TGF-1–exposed MS-5 cells had substantially lower efficiency in promoting integrin 41–mediated adhesion of NCI-H929 and Mo7e cells to soluble vascular cell adhesion molecule-1 (sVCAM-1) and CS-1/fibronectin than their untreated counterparts. Moreover, human cord blood CD34+ hematopoietic progenitor cells displayed SDF-1–dependent reduced responses in chemotaxis, transendothelial migration, and up-regulation of adhesion to sVCAM-1 when supernatants from TGF-1–treated MS-5 cells were used compared with supernatants from untreated cells. These data indicate that TGF-1–controlled reduction in SDF-1 expression influences BM cell migration and adhesion, which could affect the motility of cells trafficking the bone marrow. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Salogni, T. Musso, D. Bosisio, M. Mirolo, V. R. Jala, B. Haribabu, M. Locati, and S. 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