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Prepublished online as a Blood First Edition Paper on June 5, 2003; DOI 10.1182/blood-2002-07-1972. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-07-1972v1 102/6/2156    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zloza, A. Articles by Al-Harthi, L. Search for Related Content PubMed PubMed Citation Articles by Zloza, A. Articles by Al-Harthi, L. Related Collections Immunobiology Chemokines, Cytokines, and Interleukins Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 September 2003, Vol. 102, No. 6, pp. 2156-2164 IMMUNOBIOLOGY CD8+ T cells that express CD4 on their surface (CD4dimCD8bright T cells) recognize an antigen-specific target, are detected in vivo, and can be productively infected by T-tropic HIV Andrew Zloza, Yvonne B. Sullivan, Elizabeth Connick, Alan L. Landay, and Lena Al-Harthi From the Department of Immunology and Microbiology, Rush-Presbyterian-St Luke's Medical Center, Rush University, Chicago, IL; and the Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver. CD4 can be up-regulated on CD8+ T cells generating a CD4dimCD8bright phenotype. We previously demonstrated that the CD4dimCD8bright phenotype constitutes an activated phenotype of CD8+ T cells. We demonstrate here that the activated CD4dimCD8bright T cells are not undergoing apoptosis and do not produce significant intracellular levels of interferon (IFN), interleukin 2 (IL-2), or IL-10 but express elevated levels of intracellular IL-4 in comparison to CD8+CD4– and CD4+ T cells. In response to cytomegalovirus (CMV) peptide (pp65) priming, CD4dimCD8bright cells recognized CMV pp65 tetramer approximately 19-fold higher than CD4–CD8+ T cells, indicating that these cells are capable of antigen-specific recognition to a far greater extent than CD4–CD8+ T cells. CD4dimCD8bright T cells also express both CXCR4 and CCR5 but are susceptible to T-tropic and not M-tropic HIV infection. A soluble factor believed to be -chemokine is responsible for the inhibition of M-tropic HIV infection in CD4dimCD8bright T cells. CD8+ T cells from HIV+ patients were capable of up-regulating CD4 on CD8+ T cells. We also provide evidence of the presence of peripheral blood CD4dimCD8bright T cells in HIV+ patients, albeit at low frequency. Collectively, these data suggest a role of CD4dimCD8bright T cells in both normal T-cell biology and HIV pathogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Mutation analysis of the transcription factor PU.1 in younger adults (16 to 60 years) with acute myeloid leukemia: a study of the AML Study Group Ulm (AMLSG ULM) Konstanze Döhner, Karen Tobis, Tobias Bischof, Stefan Hein, Richard F. Schlenk, Stefan Fröhling, Hartmut Döhner, Beatrice U. Mueller, Thomas Pabst, Motomi Osato, Norio Asou, Lisa M. Johansen, Tajhal Dayaram, Gerhard Behre, Wolfgang Hiddemann, Yoshiaki Ito, and Daniel G. Tenen Blood 2003 102: 3850-3851. [Full Text] [PDF] This article has been cited by other articles: A. Zloza, J. M. Schenkel, A. R. Tenorio, J. A. Martinson, P. M. Jeziorczak, and L. Al-Harthi Potent HIV-specific responses are enriched in a unique subset of CD8+ T cells that coexpresses CD4 on its surface Blood, October 29, 2009; 114(18): 3841 - 3853. [Abstract] [Full Text] [PDF] X. Wang, A. Das, A. A. Lackner, R. S. Veazey, and B. Pahar Intestinal double-positive CD4+CD8+ T cells of neonatal rhesus macaques are proliferating, activated memory cells and primary targets for SIVMAC251 infection Blood, December 15, 2008; 112(13): 4981 - 4990. [Abstract] [Full Text] [PDF] A. Zloza and L. Al-Harthi Multiple populations of T lymphocytes are distinguished by the level of CD4 and CD8 coexpression and require individual consideration J. Leukoc. Biol., January 1, 2006; 79(1): 4 - 6. [Full Text] [PDF] A. Cochrane, S. Imlach, C. Leen, G. Scott, D. Kennedy, and P. Simmonds High Levels of Human Immunodeficiency Virus Infection of CD8 Lymphocytes Expressing CD4 In Vivo J. Virol., September 15, 2004; 78(18): 9862 - 9871. [Abstract] [Full Text] [PDF] S. J. Potter, P. Lemey, G. Achaz, C. B. Chew, A.-M. Vandamme, D. E. Dwyer, and N. K. Saksena HIV-1 compartmentalization in diverse leukocyte populations during antiretroviral therapy J. Leukoc. Biol., September 1, 2004; 76(3): 562 - 570. [Abstract] [Full Text] [PDF] M. Nascimbeni, E.-C. Shin, L. Chiriboga, D. E. Kleiner, and B. Rehermann Peripheral CD4+CD8+ T cells are differentiated effector memory cells with antiviral functions Blood, July 15, 2004; 104(2): 478 - 486. [Abstract] [Full Text] [PDF] S. G. Kitchen, N. R. Jones, S. LaForge, J. K. Whitmire, B.-A. Vu, Z. Galic, D. G. Brooks, S. J. Brown, C. M. R. Kitchen, and J. A. Zack CD4 on CD8+ T cells directly enhances effector function and is a target for HIV infection PNAS, June 8, 2004; 101(23): 8727 - 8732. [Abstract] [Full Text] [PDF]

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