|
|
Prepublished online as a Blood First Edition Paper on May 29, 2003; DOI 10.1182/blood-2002-10-3312.
 Previous Article | Table of Contents | Next Article 
Blood, 15 September 2003, Vol. 102, No. 6, pp. 2243-2250
PHAGOCYTES
Role of macrophages in the generation of circulating blood nucleosomes from dead and dying cells
Ning Jiang,
Charles F. Reich, III, and
David S. Pisetsky
From the Division of Rheumatology, Duke University Medical Center, Durham; and the Veterans Administration Hospital, Durham, NC.
After apoptosis or necrosis, macrophages clear dead cells by phagocytosis. Although this process is efficient, circulating nucleosomes can occur in certain diseases, presumably reflecting either increased production or impaired clearance. To investigate the generation of blood nucleosomes, graded numbers of apoptotic and necrotic cells were administered to healthy mice, and levels of blood nucleosomes and DNA were determined. Using Jurkat cells as a model, nucleosomes and DNA were detected in the blood after the administration of 108 apoptotic or necrotic cells per mouse by the intraperitoneal route. The kinetics of the response were similar for both types of cells. The role of macrophages was assessed by eliminating these cells with clodronate liposomes or silica. Although clodronate treatment alone produced a peak level of blood DNA, the subsequent administration of dead cells caused no change in DNA levels. In contrast, silica treatment alone did not elicit a blood DNA response, though this treatment limited the rise in DNA from administered cells. Molecular studies showed that the blood DNA following the administration of apoptotic or necrotic cells arose from the mouse and the Jurkat cells, and its size distribution was consistent with apoptosis. Together, these findings suggest that the generation of blood nucleosomes depends on macrophages, with apoptosis a concomitant of a high burden of dead and dying cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J.-M. Hou, A. Greystoke, L. Lancashire, J. Cummings, T. Ward, R. Board, E. Amir, S. Hughes, M. Krebs, A. Hughes, et al.
Evaluation of Circulating Tumor Cells and Serological Cell Death Biomarkers in Small Cell Lung Cancer Patients Undergoing Chemotherapy
Am. J. Pathol.,
August 1, 2009;
175(2):
808 - 816.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. O'Brien, D. F. Claxton, M. Crump, S. Faderl, T. Kipps, M. J. Keating, J. Viallet, and B. D. Cheson
Phase I study of obatoclax mesylate (GX15-070), a small molecule pan-Bcl-2 family antagonist, in patients with advanced chronic lymphocytic leukemia
Blood,
January 8, 2009;
113(2):
299 - 305.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Pisetsky
The Role of Nuclear Macromolecules in Innate Immunity
Proceedings of the ATS,
July 1, 2007;
4(3):
258 - 262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Bell, W. Jiang, C. F. Reich III, and D. S. Pisetsky
The extracellular release of HMGB1 during apoptotic cell death
Am J Physiol Cell Physiol,
December 1, 2006;
291(6):
C1318 - C1325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Denny, P. Chandaroy, P. D. Killen, R. Caricchio, E. E. Lewis, B. C. Richardson, K.-D. Lee, J. Gavalchin, and M. J. Kaplan
Accelerated Macrophage Apoptosis Induces Autoantibody Formation and Organ Damage in Systemic Lupus Erythematosus
J. Immunol.,
February 15, 2006;
176(4):
2095 - 2104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Jiang and D. S. Pisetsky
The effect of inflammation on the generation of plasma DNA from dead and dying cells in the peritoneum
J. Leukoc. Biol.,
March 1, 2005;
77(3):
296 - 302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Jiang and D. S. Pisetsky
The Effect of Dexamethasone on the Generation of Plasma DNA from Dead and Dying Cells
Am. J. Pathol.,
May 1, 2004;
164(5):
1751 - 1759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
