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Blood, 15 October 2003, Vol. 102, No. 8, pp. 2724-2727. Prepublished online as a Blood First Edition Paper on June 19, 2003; DOI 10.1182/blood-2003-01-0007. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-01-0007v1 102/8/2724    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Meissner, A. Articles by Körner, H. Search for Related Content PubMed PubMed Citation Articles by Meissner, A. Articles by Körner, H. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Cell Adhesion and Motility Gene Expression Brief Reports Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES Brief report CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress Anja Meissner, Olaf Zilles, Rosa Varona, Katrin Jozefowski, Uwe Ritter, Gabriel Marquez, Rupert Hallmann, and Heinrich Körner From the Interdisciplinary Center for Clinical Research, the Department for Experimental Medicine I of the University Erlangen-Nürnberg, Nikolaus-Fiebiger-Center for Molecular Medicine, Erlangen, Germany; the Departamento de Inmunología y Oncología, Centro Nacional de Biotecnología, Universidad Autónoma, Madrid, Spain; and the Department of Experimental Pathology, Lund University, Sweden. Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)–activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein–mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor down-regulation and reduced B-cell adhesion. We conclude that CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. K. Lundy, S. A. Lira, J. J. Smit, D. N. Cook, A. A. Berlin, and N. W. Lukacs Attenuation of Allergen-Induced Responses in CCR6-/- Mice Is Dependent upon Altered Pulmonary T Lymphocyte Activation J. Immunol., February 15, 2005; 174(4): 2054 - 2060. [Abstract] [Full Text] [PDF] U. Ritter, F. Wiede, D. Mielenz, Z. Kiafard, J. Zwirner, and H. Korner Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells J. Leukoc. Biol., August 1, 2004; 76(2): 472 - 476. [Abstract] [Full Text] [PDF]

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