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Blood, 15 October 2003, Vol. 102, No. 8, pp. 2803-2810. Prepublished online as a Blood First Edition Paper on July 3, 2003; DOI 10.1182/blood-2003-01-0306. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-01-0306v1 102/8/2803    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bijnens, A. P. J. J. Articles by Cleutjens, K. B. J. M. Search for Related Content PubMed PubMed Citation Articles by Bijnens, A. P. J. J. Articles by Cleutjens, K. B. J. M. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Vasculin, a novel vascular protein differentially expressed in human atherogenesis Ann P. J. J. Bijnens, Ann Gils, Barry Jutten, Birgit C. G. Faber, Sylvia Heeneman, Peter J. E. H. M. Kitslaar, Jan H. M. Tordoir, Carlie J. M. de Vries, Abraham A. Kroon, Mat J. A. P. Daemen, and Kitty B. J. M. Cleutjens From the Departments of Pathology, General Surgery, and Internal Medicine Cardiovascular Research Institute Maastricht (CARIM), University of Maastricht, the Netherlands; and Department of Biochemistry, Academical Medical Center, University of Amsterdam, The Netherlands. Recent suppressive subtractive hybridization analysis on human atherosclerotic plaque-derived RNA revealed genes upregulated in plaques with a thrombus versus stable plaques. Clone SSH6, containing part of a putative open reading frame of an unknown protein, was further investigated. Full-length cDNA, coding for a 473–amino acid (aa) protein, was identified in a vascular smooth muscle cell (SMC) cDNA library. Bioinformatics suggested the presence of multiple SSH6 variants due to alternative splicing of exon 3. Multiple-tissue Northern blot analysis demonstrated a differential expression pattern of these variants, as a ubiquitously expressed SSH6 mRNA missing exon 3, was detected apart from a putative vascular SMC–specific form containing exon 3. Western blot analysis indicated a ubiquitous 35-kDa protein (SSH6-), in addition to a 45-kDa protein (vasculin), detected in the vascular wall and in plasma. Analysis of arteries displaying various stages of atherosclerosis indicated that the vasculin/SSH6- ratio increases throughout atherogenesis. Immunohistochemical analysis demonstrated cytoplasmic expression of SSH6 gene products in macrophages, endothelial cells, and SMCs. In summary, we identified a novel mRNA/protein, vasculin, in the arterial wall and plasma. The regulated expression of vasculin in plaques suggests a role in atherogenesis. Moreover, its presence in plasma opens perspectives for vasculin as a marker for atherosclerosis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A.P.J.J. Bijnens, E. Lutgens, T. Ayoubi, J. Kuiper, A.J. Horrevoets, and M.J.A.P. Daemen Genome-Wide Expression Studies of Atherosclerosis: Critical Issues in Methodology, Analysis, Interpretation of Transcriptomics Data Arterioscler Thromb Vasc Biol, June 1, 2006; 26(6): 1226 - 1235. [Abstract] [Full Text] [PDF] E. Lutgens, B. Faber, K. Schapira, C. T.A. Evelo, R. van Haaften, S. Heeneman, K. B.J.M. Cleutjens, A. P. Bijnens, L. Beckers, J. G. Porter, et al. Gene Profiling in Atherosclerosis Reveals a Key Role for Small Inducible Cytokines: Validation Using a Novel Monocyte Chemoattractant Protein Monoclonal Antibody Circulation, June 28, 2005; 111(25): 3443 - 3452. [Abstract] [Full Text] [PDF] E. Lutgens, R.-J. van Suylen, B. C. Faber, M. J. Gijbels, P. M. Eurlings, A.-P. Bijnens, K. B. Cleutjens, S. Heeneman, and M. J.A.P. Daemen Atherosclerotic Plaque Rupture: Local or Systemic Process? Arterioscler Thromb Vasc Biol, December 1, 2003; 23(12): 2123 - 2130. [Abstract] [Full Text] [PDF]

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