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Blood, 15 October 2003, Vol. 102, No. 8, pp. 2940-2947. Prepublished online as a Blood First Edition Paper on July 3, 2003; DOI 10.1182/blood-2002-12-3651. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-12-3651v1 102/8/2940    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Granucci, F. Articles by Ricciardi-Castagnoli, P. Search for Related Content PubMed PubMed Citation Articles by Granucci, F. Articles by Ricciardi-Castagnoli, P. Related Collections Immunobiology Phagocytes Cytoskeleton Signal Transduction Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia Francesca Granucci, Filippo Petralia, Matteo Urbano, Stefania Citterio, Francesco Di Tota, Laura Santambrogio, and Paola Ricciardi-Castagnoli From the Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milan, Italy; and the Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA. Macrophage receptor with collagenous structure (MARCO) is a scavenger receptor expressed in peritoneal macrophages and in a subpopulation of macrophages in the marginal zone of the spleen and in the medullary cord of lymph nodes. By global gene expression analysis, it has been found that the MARCO mRNA was one of the most up-regulated in splenic dendritic cells (DCs) following lipopolysaccharide or bacterial activation and in granulocyte-macrophage colony-stimulating factor (GM-CSF)–treated microglial cells. Here we show that MARCO is expressed on splenic DCs at late time points after activation and that its expression correlates with profound changes in actin cytoskeleton organization in DCs and microglia. During maturation, DCs undergo profound rearrangements of actin cytoskeleton. Immature DCs are adherent with visible actin cables, while fully mature, MARCO-expressing, splenic DCs are nonadherent, round in shape, and have an actin cytoskeleton with a punctate distribution. The simple expression of MARCO was sufficient to induce these cytoskeleton modifications in DCs. MARCO-transfected immature DCs acquired a typical morphology of mature DCs and did not rearrange the actin cytoskeleton following activation. Moreover, DCs in which MARCO was knocked down did not reach the mature phenotype and maintained the typical morphology of transitional DCs. MARCO expression in DCs and microglial cells was also associated with a decrease of antigen internalization capacity. Thus, the MARCO receptor is important for actin cytoskeleton rearrangements and the down-regulation of antigen uptake function during DC and microglial cell maturation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. M. Reddy, V. Suryanarayana, D. V. Kalvakolanu, M. Yamamoto, T. W. Kensler, P. M. Hassoun, S. R. Kleeberger, and S. P. Reddy Innate Immunity against Bacterial Infection following Hyperoxia Exposure Is Impaired in NRF2-Deficient Mice J. Immunol., October 1, 2009; 183(7): 4601 - 4608. 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