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Blood, 1 November 2003, Vol. 102, No. 9, pp. 3136-3146. Prepublished online as a Blood First Edition Paper on July 17, 2003; DOI 10.1182/blood-2003-03-0923. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-03-0923v1 102/9/3136    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kolbus, A. Articles by Beug, H. Search for Related Content PubMed PubMed Citation Articles by Kolbus, A. Articles by Beug, H. Related Collections Signal Transduction Gene Expression Genomics Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Cooperative signaling between cytokine receptors and the glucocorticoid receptor in the expansion of erythroid progenitors: molecular analysis by expression profiling Andrea Kolbus, Montserrat Blázquez-Domingo, Sebastian Carotta, Walbert Bakker, Susanna Luedemann, Marieke von Lindern, Peter Steinlein, and Hartmut Beug From the Research Institute of Molecular Pathology (IMP), Vienna, Austria; and the Institute of Hematology, Erasmus MC, Rotterdam, The Netherlands. Erythroid progenitors undergo renewal (proliferation without apparent differentiation) in response to erythropoietin (Epo), stem cell factor (SCF), and glucocorticoids (dexamethasone) (Dex). SCF and Dex cooperate with Epo to promote proliferation and inhibit differentiation of erythroid progenitors, while Epo alone is required to protect erythroid cells from apoptosis during terminal red cell maturation. To examine the mechanism of the synergistic interactions of Epo, SCF, and Dex, we analyzed gene expression patterns using DNA chip–based large-scale comparative gene profiling using microarrays enriched in hematopoietic transcripts or containing randomly selected genes. Differentially regulated genes were validated by real-time reverse transcription–polymerase chain reaction (RT-PCR). The results reveal cooperative regulation of gene expression by glucocorticoids and Epo/SCF on a number of genes, such as CIS, BTG1, VDUP1, CXCR4, GILZ, and RIKEN29300106B05. While Epo and SCF never showed opposite effects on gene expression, Dex either enhanced or attenuated the effect of Epo and/or SCF. Several glucocorticoid receptor (GR)–target genes were regulated by Dex only in the presence of Epo and/or SCF, suggesting that the GR functions in the context of a larger transactivation complex to regulate these genes. The data also suggest that modulation of cytokine-induced signals by the GR is an important mechanism in erythroid progenitor renewal. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: O. Tanabe, Y. Shen, Q. Liu, A. D. Campbell, T. Kuroha, M. Yamamoto, and J. D. Engel The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription Genes & Dev., November 1, 2007; 21(21): 2832 - 2844. [Abstract] [Full Text] [PDF] W. J. Bakker, T. B. van Dijk, M. Parren-van Amelsvoort, A. Kolbus, K. Yamamoto, P. Steinlein, R. G. W. Verhaak, T. W. Mak, H. Beug, B. Lowenberg, et al. Differential Regulation of Foxo3a Target Genes in Erythropoiesis Mol. Cell. Biol., May 15, 2007; 27(10): 3839 - 3854. 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