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Blood, 1 November 2003, Vol. 102, No. 9, pp. 3238-3240. Prepublished online as a Blood First Edition Paper on July 17, 2003; DOI 10.1182/blood-2003-03-0966. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-03-0966v1 102/9/3238    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ward, A. C. Articles by Lieschke, G. J. Search for Related Content PubMed PubMed Citation Articles by Ward, A. C. Articles by Lieschke, G. J. Related Collections Phagocytes Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Brief report The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish Alister C. Ward, Dora O. McPhee, Melanie M. Condron, Sony Varma, Stephen H. Cody, Sara M. N. Onnebo, Barry H. Paw, Leonard I. Zon, and Graham J. Lieschke From the Centre for Cellular and Molecular Biology, Deakin University, Burwood, Victoria, Australia; Ludwig Institute for Cancer Research, Parkville, Victoria, Australia; and Howard Hughes Medical Institute, Children's Hospital, Boston, MA. The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. To enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kilobase promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of enhanced green fluorescent protein (EGFP) in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germ line transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Lin, J. A. Loughman, B. H. Zinselmeyer, M. J. Miller, and M. G. Caparon Streptolysin S Inhibits Neutrophil Recruitment during the Early Stages of Streptococcus pyogenes Infection Infect. Immun., November 1, 2009; 77(11): 5190 - 5201. [Abstract] [Full Text] [PDF] O. W. Stockhammer, A. Zakrzewska, Z. 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