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Blood, 1 November 2003, Vol. 102, No. 9, pp. 3387-3395. Prepublished online as a Blood First Edition Paper on July 17, 2003; DOI 10.1182/blood-2003-02-0621. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-02-0621v1 102/9/3387    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carlyon, J. A. Articles by Fikrig, E. Search for Related Content PubMed PubMed Citation Articles by Carlyon, J. A. Articles by Fikrig, E. Related Collections Phagocytes Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES Murine neutrophils require 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum Jason A. Carlyon, Mustafa Akkoyunlu, Lijun Xia, Tadayuki Yago, Tian Wang, Richard D. Cummings, Rodger P. McEver, and Erol Fikrig From the Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT; Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK; and Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK. Anaplasma phagocytophilum causes human granulocytic ehrlichiosis, the second most common tick-borne disease in the United States. Mice are natural reservoirs for this bacterium and man is an inadvertent host. A phagocytophilum's tropism for human neutrophils is linked to neutrophil expression of P-selectin glycoprotein ligand-1 (PSGL-1), as well as sialylated and 1,3-fucosylated glycans. To determine whether A phagocytophilum uses similar molecular features to infect murine neutrophils, we assessed in vitro bacterial binding to neutrophils from and infection burden in wild-type mice; mice lacking 1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice lacking PSGL-1. Binding to Fuc-TIV-/-/Fuc-TVII-/- neutrophils and infection of Fuc-TIV-/-/Fuc-TVII-/- mice were significantly reduced relative to wild-type mice. A phagocytophilum binding to PSGL-1-/- neutrophils was modestly reduced, whereas sialidase treatment significantly decreased binding to both wild-type and PSGL-1-/- neutrophils. A phagocytophilum similarly infected PSGL-1-/- and wild-type mice in vivo. A phagocytophilum induced comparable levels of chemokines from wild-type and PSGL-1-/- neutrophils in vitro, while those induced from Fuc-TIV-/-/Fuc-TVII-/- neutrophils were appreciably reduced. Therefore, A phagocytophilum infection in mice, as in humans, requires sialylation and 1,3-fucosylation of neutrophils. However, murine infection does not require neutrophil PSGL-1 expression, which has important implications for understanding how A phagocytophilum binds and infects neutrophils. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. J. Troese and J. A. Carlyon Anaplasma phagocytophilum Dense-Cored Organisms Mediate Cellular Adherence through Recognition of Human P-Selectin Glycoprotein Ligand 1 Infect. Immun., September 1, 2009; 77(9): 4018 - 4027. [Abstract] [Full Text] [PDF] M. J. Troese, M. Sarkar, N. L. Galloway, R. J. Thomas, S. A. Kearns, D. V. Reneer, T. Yang, and J. A. Carlyon Differential Expression and Glycosylation of Anaplasma phagocytophilum Major Surface Protein 2 Paralogs during Cultivation in Sialyl Lewis x-Deficient Host Cells Infect. Immun., May 1, 2009; 77(5): 1746 - 1756. [Abstract] [Full Text] [PDF] M. Sarkar, D. V. Reneer, and J. A. Carlyon Sialyl-Lewis x-Independent Infection of Human Myeloid Cells by Anaplasma phagocytophilum Strains HZ and HGE1 Infect. Immun., December 1, 2007; 75(12): 5720 - 5725. [Abstract] [Full Text] [PDF] U. Blas-Machado, J. de la Fuente, E. F. Blouin, C. Almazan, K. M. Kocan, and J. V. Mysore Experimental Infection of C3H/HeJ Mice with the NY18 Isolate of Anaplasma phagocytophilum Vet. Pathol., January 1, 2007; 44(1): 64 - 73. [Abstract] [Full Text] [PDF] T. Ju, Q. Zheng, and R. D. Cummings Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans Glycobiology, October 1, 2006; 16(10): 947 - 958. [Abstract] [Full Text] [PDF] R. R. Dinglasan and M. Jacobs-Lorena Insight into a Conserved Lifestyle: Protein-Carbohydrate Adhesion Strategies of Vector-Borne Pathogens Infect. Immun., December 1, 2005; 73(12): 7797 - 7807. [Full Text] [PDF] J. A. Carlyon, D. Ryan, K. Archer, and E. Fikrig Effects of Anaplasma phagocytophilum on Host Cell Ferritin mRNA and Protein Levels Infect. Immun., November 1, 2005; 73(11): 7629 - 7636. [Abstract] [Full Text] [PDF] J. A. Carlyon, D. A. Latif, M. Pypaert, P. Lacy, and E. Fikrig Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection Infect. Immun., August 1, 2004; 72(8): 4772 - 4783. [Abstract] [Full Text] [PDF]

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