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Blood, 1 November 2003, Vol. 102, No. 9, pp. 3396-3403. Prepublished online as a Blood First Edition Paper on July 10, 2003; DOI 10.1182/blood-2003-01-0151. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-01-0151v1 102/9/3396    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yang, D. Articles by Oppenheim, J. J. Search for Related Content PubMed PubMed Citation Articles by Yang, D. Articles by Oppenheim, J. J. Related Collections Immunobiology Phagocytes Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells De Yang, Helene F. Rosenberg, Qian Chen, Kimberly D. Dyer, Kahori Kurosaka, and Joost J. Oppenheim From the Basic Research Program, SAIC-Frederick, MD; Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD; Eosinophil Pathophysiology Section, Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD. Recent publications have highlighted the chemotactic activities of antimicrobial proteins derived from the granules of neutrophils and basophils. Eosinophil granules also contain antimicrobial proteins. One of them is eosinophil-derived neurotoxin (EDN), a protein belonging to the ribonuclease A (RNase A) superfamily, which has recently been found to have antiviral activity in vitro. We found that EDN was selectively chemotactic for dendritic cells (DCs). The DC chemotactic activity of EDN was inhibited by either pretreatment of DCs with pertussis toxin or by simultaneous addition of placental RNase inhibitor to inhibit the activity of EDN. EDN was not chemotactic for leukocytes other than DCs. Mouse eosinophilassociated RNase 2 (mEAR2), one of a cluster of divergent orthologs of human EDN, was also chemotactic for human as well as mouse DCs. Sequence and mutational analysis demonstrated the importance of the N-terminal region of mEAR2 in mediating its chemotactic effect on DCs. EDN also induced the activation of p42/44 mitogen-activated protein kinase (MAPK) in DCs. Furthermore, injection of mEAR2 into the air pouches of mice resulted in the recruitment of DCs into the air pouches. Thus, EDN and its mouse ortholog, mEAR2, are eosinophil granule–derived antimicrobial RNases that function as chemoattractants for DCs in vitro and in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. F. Rosenberg RNase A ribonucleases and host defense: an evolving story J. Leukoc. Biol., May 1, 2008; 83(5): 1079 - 1087. [Abstract] [Full Text] [PDF] D. Yang, Q. Chen, S. B. Su, P. Zhang, K. Kurosaka, R. R. Caspi, S. M. Michalek, H. F. Rosenberg, N. Zhang, and J. J. Oppenheim Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2-MyD88 signal pathway in dendritic cells and enhances Th2 immune responses J. Exp. Med., January 21, 2008; 205(1): 79 - 90. [Abstract] [Full Text] [PDF] J. Harder, R. Glaser, and J.-M. Schroder Review: Human antimicrobial proteins effectors of innate immunity Innate Immunity, December 1, 2007; 13(6): 317 - 338. [Abstract] [PDF] T. Nitto, K. D. Dyer, M. Czapiga, and H. F. Rosenberg Evolution and Function of Leukocyte RNase A Ribonucleases of the Avian Species, Gallus gallus J. Biol. Chem., September 1, 2006; 281(35): 25622 - 25634. [Abstract] [Full Text] [PDF] N. Zhang, D. Yang, H. Dong, Q. Chen, D. I. Dimitrova, T. J. Rogers, M. Sitkovsky, and J. J. Oppenheim Adenosine A2a receptors induce heterologous desensitization of chemokine receptors Blood, July 1, 2006; 108(1): 38 - 44. [Abstract] [Full Text] [PDF] T. L. Garvey, K. D. Dyer, J. A. Ellis, C. A. Bonville, B. Foster, C. Prussin, A. J. Easton, J. B. Domachowske, and H. F. Rosenberg Inflammatory Responses to Pneumovirus Infection in IFN-{alpha}{beta}R Gene-Deleted Mice J. Immunol., October 1, 2005; 175(7): 4735 - 4744. [Abstract] [Full Text] [PDF] K. Kurosaka, Q. Chen, F. Yarovinsky, J. J. Oppenheim, and D. Yang Mouse Cathelin-Related Antimicrobial Peptide Chemoattracts Leukocytes Using Formyl Peptide Receptor-Like 1/Mouse Formyl Peptide Receptor-Like 2 as the Receptor and Acts as an Immune Adjuvant J. Immunol., May 15, 2005; 174(10): 6257 - 6265. [Abstract] [Full Text] [PDF] D. Yang, Q. Chen, H. F. Rosenberg, S. M. Rybak, D. L. Newton, Z. Y. Wang, Q. Fu, V. T. Tchernev, M. Wang, B. Schweitzer, et al. Human Ribonuclease A Superfamily Members, Eosinophil-Derived Neurotoxin and Pancreatic Ribonuclease, Induce Dendritic Cell Maturation and Activation J. Immunol., November 15, 2004; 173(10): 6134 - 6142. [Abstract] [Full Text] [PDF] O. Levy Antimicrobial proteins and peptides: anti-infective molecules of mammalian leukocytes J. Leukoc. Biol., November 1, 2004; 76(5): 909 - 925. [Abstract] [Full Text] [PDF] Q. Chen, D. Wade, K. Kurosaka, Z. Y. Wang, J. J. Oppenheim, and D. 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