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Blood, 1 January 2004, Vol. 103, No. 1, pp. 188-193. Prepublished online as a Blood First Edition Paper on July 24, 2003; DOI 10.1182/blood-2003-05-1487. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-05-1487v1 103/1/188    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marafioti, T. Articles by Mason, D. Y. Search for Related Content PubMed PubMed Citation Articles by Marafioti, T. Articles by Mason, D. Y. Related Collections Neoplasia Signal Transduction Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Expression of intracellular signaling molecules in classical and lymphocyte predominance Hodgkin disease Teresa Marafioti, Michela Pozzobon, Martin-Leo Hansmann, Georges Delsol, Stefano A. Pileri, and David Y. Mason From the Nuffield Department of Clinical Laboratory Sciences and Leukaemia Research Fund Immunodiagnostics Unit, John Radcliffe Hospital, Oxford, United Kingdom; Senckenbergisches Institut für Pathologie, Klinikum der Johann Wolfgang Goethe-Universität, Frankfurt, Germany; Centre de Physiopathologie de Toulouse Institut National de la Santé et de la Recherche Médicale (INSERM) (U 563), Purpan, Toulouse, France; and Cattedra di Anatomia Patologica, Servizio di Ematopatologia, Istituto di Ematologia ed Oncologia Medica "L. e A. Seragnoli," Policlinico S. Orsola, Bologna, Italy. The neoplastic cells in classical Hodgkin disease (Reed-Sternberg cells) are of B-lymphoid origin, but they lack many markers of this cell lineage, for example, immunoglobulin, CD20, and B-cell–associated transcription factors. In contrast, the neoplastic cells ("L&H" cells) in lymphocyte predominance Hodgkin disease retain the molecular profile of germinal center B cells. In this paper, we investigated the expression in Hodgkin disease (45 cases and 3 cell lines) of 5 intracellular signaling molecules found in B cells. The Src family kinase Syk, the B-cell adaptor protein BLNK, and phospholipase C (PLC)–2 were consistently absent from Reed-Sternberg cells, whereas 2 other Src kinases (Lyn and Fyn) were heterogeneously expressed in a proportion of cases (12% and 42%, respectively). In contrast, the tumor cells in all cases of lymphocyte predominance Hodgkin disease were positive for Fyn, Syk, BLNK, and PLC-2, and Lyn immunostaining was seen in a minority of biopsies. These results indicate that in Reed-Sternberg cells, the defect in B-cell lineage marker expression includes a spectrum of molecules involved in intracellular signaling, a finding in keeping with recent gene expression profiling studies. Furthermore, the clear difference in expression of signaling proteins between the 2 major subtypes of Hodgkin disease may be of diagnostic value. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Marafioti, J. C. Paterson, E. Ballabio, K. K. Reichard, S. Tedoldi, K. Hollowood, M. Dictor, M.-L. Hansmann, S. A. Pileri, M. J. Dyer, et al. Novel markers of normal and neoplastic human plasmacytoid dendritic cells Blood, April 1, 2008; 111(7): 3778 - 3792. [Abstract] [Full Text] [PDF] G. Mattsson, S. Y. Tan, D. J.P. Ferguson, W. Erber, S. H. Turner, T. Marafioti, and D. Y. 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[Abstract] [Full Text] [PDF] S. Tedoldi, J. C. Paterson, M.-L. Hansmann, Y. Natkunam, T. Rudiger, P. Angelisova, M. Q. Du, H. Roberton, G. Roncador, L. Sanchez, et al. Transmembrane adaptor molecules: a new category of lymphoid-cell markers Blood, January 1, 2006; 107(1): 213 - 221. [Abstract] [Full Text] [PDF] Y. Natkunam, I. S. Lossos, B. Taidi, S. Zhao, X. Lu, F. Ding, A. S. Hammer, T. Marafioti, G. E. Byrne Jr, S. Levy, et al. Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation Blood, May 15, 2005; 105(10): 3979 - 3986. [Abstract] [Full Text] [PDF]

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