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Blood, 1 January 2004, Vol. 103, No. 1, pp. 222-228. Prepublished online as a Blood First Edition Paper on September 11, 2003; DOI 10.1182/blood-2003-05-1538. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-05-1538v1 103/1/222    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by An, J. Articles by Rettig, M. B. Search for Related Content PubMed PubMed Citation Articles by An, J. Articles by Rettig, M. B. Related Collections Neoplasia Oncogenes and Tumor Suppressors Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Transcriptional coactivation of c-Jun by the KSHV-encoded LANA Jiabin An, Yiping Sun, and Matthew B. Rettig From the VA Greater Los Angeles Healthcare System, Los Angeles, CA; and Department of Medicine, UCLA School of Medicine, Los Angeles, CA. The Kaposi sarcoma–associated herpesvirus (KSHV)–encoded latency-associated nuclear antigen (LANA) modulates viral and cellular gene expression, including interleukin 6 (IL-6), a growth factor for KSHV-associated diseases. LANA-driven IL-6 expression is dependent on the activator protein 1 (AP1) response element (RE) within the IL-6 promoter. We show that LANA activates the AP1 RE in a Jun-dependent fashion and that LANA enhances the transcriptional activity of a GAL4-Jun fusion protein. Coimmunoprecipitation studies documented a physical interaction between LANA and c-Jun in transiently transfected 293 cells as well as the KSHV-infected BCBL-1 primary effusion lymphoma (PEL) cell line. Taken together, these data indicate that LANA is a transcriptional coactivator of c-Jun. In addition, electrophoretic mobility shift assays demonstrated that LANA induces binding of a c-Jun-Fos heterodimer to the AP1 RE, but does not itself bind to the AP1 RE. RNA interference experiments confirmed that LANA activates the AP1 RE, stimulates binding of a c-Jun-Fos heterodimer to the AP1 RE, and induces expression of IL-6. These data indicate that LANA is a transcriptional coactivator of c-Jun, a function that may have implications for the pathogenesis of KSHV-associated diseases. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Lu, S. C. Verma, M. Murakami, Q. Cai, P. Kumar, B. Xiao, and E. S. 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Hernandez, C. Corriveau-Bourque, L. M. Fawaz, J. Hiscott, and R. Lin Leukotriene A4 Hydrolase Expression in PEL Cells Is Regulated at the Transcriptional Level and Leads to Increased Leukotriene B4 Production. J. Immunol., June 1, 2006; 176(11): 7051 - 7061. [Abstract] [Full Text] [PDF] M. Fujimuro, J. Liu, J. Zhu, H. Yokosawa, and S. D. Hayward Regulation of the Interaction between Glycogen Synthase Kinase 3 and the Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen J. Virol., August 15, 2005; 79(16): 10429 - 10441. [Abstract] [Full Text] [PDF] S. M. DeWire and B. Damania The Latency-Associated Nuclear Antigen of Rhesus Monkey Rhadinovirus Inhibits Viral Replication through Repression of Orf50/Rta Transcriptional Activation J. Virol., March 1, 2005; 79(5): 3127 - 3138. [Abstract] [Full Text] [PDF] L.-Y. Wong, G. A. Matchett, and A. C. Wilson Transcriptional Activation by the Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Is Facilitated by an N-Terminal Chromatin-Binding Motif J. Virol., September 15, 2004; 78(18): 10074 - 10085. [Abstract] [Full Text] [PDF]

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