SEARCH:   Advanced

Blood, 1 January 2004, Vol. 103, No. 1, pp. 313-316. Prepublished online as a Blood First Edition Paper on September 11, 2003; DOI 10.1182/blood-2003-05-1710. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-05-1710v1 103/1/313    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Trivedi, P. Articles by Faggioni, A. Search for Related Content PubMed PubMed Citation Articles by Trivedi, P. Articles by Faggioni, A. Related Collections Immunobiology Neoplasia Gene Expression Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Brief report Infection of HHV-8+ primary effusion lymphoma cells with a recombinant Epstein-Barr virus leads to restricted EBV latency, altered phenotype, and increased tumorigenicity without affecting TCL1 expression Pankaj Trivedi, Kumi Takazawa, Claudia Zompetta, Laura Cuomo, Elena Anastasiadou, Antonino Carbone, Stefania Uccini, Filippo Belardelli, Kenzo Takada, Luigi Frati, and Alberto Faggioni From the Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Experimental Medicine and Pathology, University of Rome, "La Sapienza," Rome, Italy; Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan; Department of Clinical Pathology, San Filippo Neri Hospital, Rome, Italy; Division of Pathology and Scientific Direction, Centro di Riferimento Oncologico-IRCCS, National Cancer Institute, Aviano, Italy; Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy; and INM, Neuromed, Pozzilli (IS), Italy. To investigate the role of Epstein-Barr virus (EBV) in the pathogenesis of primary effusion lymphoma (PEL), we infected human herpesvirus 8 (HHV-8+) but EBV- PEL lines BC-3, CRO-AP/6, and CRO-AP/3 cells with the recombinant Akata EBV strain. All EBV-infected clones expressed EBER-1, EBNA-1, and LMP2A. The expression of LMP1 and LMP2B was variable. None, however, expressed EBNA2-6. The surface markers CD30, CD74, and syndecan-1 were down-regulated in EBV convertants. EBV-infected BC-3 and CRO-AP/6 cells were highly tumorigenic in severe combined immunodeficiency (SCID) mice in contrast to their respective EBV- parental cells. However, neither the parental cells nor the virusconverted counterparts expressed TCL1. The results showing that PEL cells on in vitro EBV infection do not sustain latency III despite the absence of immune pressure indicate that the choice of EBV latent gene expression program is cell dependent. The data suggest an important role of EBV in the pathogenesis of PEL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A. Mack and B. Sugden EBV Is Necessary for Proliferation of Dually Infected Primary Effusion Lymphoma Cells Cancer Res., September 1, 2008; 68(17): 6963 - 6968. [Abstract] [Full Text] [PDF] D. Xu, T. Coleman, J. Zhang, A. Fagot, C. Kotalik, L. Zhao, P. Trivedi, C. Jones, and L. Zhang Epstein-Barr Virus Inhibits Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication in Primary Effusion Lymphomas J. Virol., June 1, 2007; 81(11): 6068 - 6078. [Abstract] [Full Text] [PDF] E. Boulanger, L. Gerard, J. Gabarre, J.-M. Molina, C. Rapp, J.-F. Abino, J. Cadranel, S. Chevret, and E. Oksenhendler Prognostic Factors and Outcome of Human Herpesvirus 8-Associated Primary Effusion Lymphoma in Patients With AIDS J. Clin. Oncol., July 1, 2005; 23(19): 4372 - 4380. [Abstract] [Full Text] [PDF] A. Farina, R. Feederle, S. Raffa, R. Gonnella, R. Santarelli, L. Frati, A. Angeloni, M. R. Torrisi, A. Faggioni, and H.-J. Delecluse BFRF1 of Epstein-Barr Virus Is Essential for Efficient Primary Viral Envelopment and Egress J. Virol., March 15, 2005; 79(6): 3703 - 3712. [Abstract] [Full Text] [PDF] W. Fan, D. Bubman, A. Chadburn, W. J. Harrington Jr., E. Cesarman, and D. M. Knowles Distinct Subsets of Primary Effusion Lymphoma Can Be Identified Based on Their Cellular Gene Expression Profile and Viral Association J. Virol., January 15, 2005; 79(2): 1244 - 1251. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020