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Blood, 1 January 2004, Vol. 103, No. 1, pp. 50-57. Prepublished online as a Blood First Edition Paper on July 24, 2003; DOI 10.1182/blood-2003-03-0909. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-03-0909v1 103/1/50    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Socié, G. Articles by Janin, A. Search for Related Content PubMed PubMed Citation Articles by Socié, G. Articles by Janin, A. Related Collections Transplantation Apoptosis Clinical Trials and Observations Immunobiology Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CLINICAL OBSERVATIONS, INTERVENTIONS, AND THERAPEUTIC TRIALS Prognostic value of apoptotic cells and infiltrating neutrophils in graft-versus-host disease of the gastrointestinal tract in humans: TNF and Fas expression Gérard Socié, Jean-Yves Mary, Marc Lemann, Marjan Daneshpouy, Philippe Guardiola, Véronique Meignin, Lionel Ades, Hélène Esperou, Patricia Ribaud, Agnès Devergie, Eliane Gluckman, Jean-Claude Ameisen, and Anne Janin From the Service d'Hématologie Greffe de Moelle, INSERM ERM-0220, INSERM ERM-0321, Service d'Hépato-gastroentérologie, Service de Pathologie, and INSERM EMI 9922, Université Paris VII and Hôpital Saint Louis and Bichat, Paris, France. The gastrointestinal (GI) tract is a major target in graft-versus-host disease (GvHD). In rodents both tumor necrosis factor (TNF) and Fas-dependent apoptosis have been shown to play a major role in GvHD lesions, but data in humans on TNF and Fas in situ expression are scarce. More recently, the role of non-T cells as GvHD effectors has also been suggested in experimental models. Here we report a detailed quantitative pathologic analysis in 95 patients who underwent gastroduodenal biopsy. This analysis included characterization and quantification of the cellular infiltrate, TNF, TNF receptors, and Fas in situ expression analyses and quantification of apoptotic cell numbers. TNF was expressed in all biopsies and it was highly specific for acute GvHD. In multivariate analysis, including pathologic factors only, increased early transplantrelated mortality (TRM) was associated with the presence of more than 20 neutrophils per field. Factors affecting early and late TRM were then assessed by multivariate analyses including both pathologic and clinical factors. Increased day-90 TRM was associated with the presence of more than 5 apoptotic bodies per field within the cellular infiltrate, and with stage II or higher acute liver GvHD. One-year TRM associated with the same 2 factors and with chronic GvHD. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Luft, M. Conzelmann, A. Benner, M. Rieger, M. Hess, U. Strohhaecker, M. Gorner, U. Hegenbart, A. D. Ho, and P. Dreger Serum cytokeratin-18 fragments as quantitative markers of epithelial apoptosis in liver and intestinal graft-versus-host disease Blood, December 15, 2007; 110(13): 4535 - 4542. [Abstract] [Full Text] [PDF] M. Ertault-Daneshpouy, C. Leboeuf, M. Lemann, F. Bouhidel, L. Ades, E. Gluckman, G. Socie, and A. Janin Pericapillary hemorrhage as criterion of severe human digestive graft-versus-host disease Blood, June 15, 2004; 103(12): 4681 - 4684. [Abstract] [Full Text] [PDF]

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