SEARCH:   Advanced

Blood, 1 June 2004, Vol. 103, No. 11, pp. 4294-4301. Prepublished online as a Blood First Edition Paper on February 12, 2004; DOI 10.1182/blood-2003-08-2688. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-08-2688v1 103/11/4294    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rosenbauer, F. Articles by Tenen, D. G. Search for Related Content PubMed PubMed Citation Articles by Rosenbauer, F. Articles by Tenen, D. G. Related Collections Phagocytes Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES pDP4, a novel glycoprotein secreted by mature granulocytes, is regulated by transcription factor PU.1 Frank Rosenbauer, Katharina Wagner, Pu Zhang, Klaus-Peter Knobeloch, Atsushi Iwama, and Daniel G. Tenen From the Harvard Institutes of Medicine, Harvard Medical School, Boston, MA; Department of Molecular Genetics, Institute of Molecular Pharmacology, Berlin, Germany; and Laboratory of Stem Cell Therapy, Center for Experimental Medicine, The Institute of Medical Science, University of Tokyo, Japan. The transcription factor PU.1 (Spi-1) is a well-characterized regulator of myeloid and lymphoid development. However, its role in mature functional cells is poorly studied. Here we report the characterization of the novel murine gene pDP4 (PU.1 difference product 4), which is absent from fetal livers of PU.1-deficient mice. pDP4 is transcribed as a single 3.2-kb mRNA with a 1518-base pair open reading frame encoded by 5 exons on chromosome 14. pDP4 expression is strongest in small intestine and bone marrow, in which it is expressed predominately in mature neutrophils. Interestingly, however, pDP4 expression is markedly down-regulated in neutrophils of the peripheral blood and peritoneum. The pDP4 gene encodes a secreted 57-kDa glycoprotein with an olfactomedin-like C-terminus. PU.1 binds to a functional site within the pDP4 promoter, and pDP4 expression in myeloid cells is strictly dependent on PU.1 and the presence of this site. In conclusion, we have identified a novel PU.1-regulated extracellular glycoprotein of the olfactomedin-like family with a possible role in neutrophilic trafficking. (Blood. 2004;103:4294-4301) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: O. Grinchuk, Z. Kozmik, X. Wu, and S. Tomarev The Optimedin Gene Is a Downstream Target of Pax6 J. Biol. Chem., October 21, 2005; 280(42): 35228 - 35237. [Abstract] [Full Text] [PDF] Y. Okuno, G. Huang, F. Rosenbauer, E. K. Evans, H. S. Radomska, H. Iwasaki, K. Akashi, F. Moreau-Gachelin, Y. Li, P. Zhang, et al. Potential Autoregulation of Transcription Factor PU.1 by an Upstream Regulatory Element Mol. Cell. Biol., April 1, 2005; 25(7): 2832 - 2845. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020