|
|
Blood, 1 June 2004, Vol. 103, No. 11, pp. 4310-4316.
Prepublished online as a Blood First Edition Paper on February 19, 2004; DOI 10.1182/blood-2003-09-3362.
 Previous Article | Table of Contents | Next Article 
RED CELLS
Caspase-3 has a nonapoptotic function in erythroid maturation
Graeme W. Carlile,
Deborah H. Smith, and
Martin Wiedmann
From the Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY; and Weill Graduate School of Medical Sciences of Cornell University, New York, NY.
Caspase-3 plays a central role in apoptosis. It is also activated in normal erythropoiesis, with its activity peaking early during development (erythroid colony-forming unit [CFU-E] stage). In the present study, we have reduced the expression and subsequent enzymatic activity of caspase-3 by transfection of small interfering RNA (siRNA) directed to caspase-3 in a differentiating human erythroid culture system. We find that siRNA treatment yields a 50% reduction in cells that undergo enucleation with no change in the fraction of cells that undergo apoptosis, measured throughout the culture. Furthermore, a substantial fraction of treated cells are unable to complete the transition from pronormoblasts to basophilic normoblasts. These results demonstrate that caspase-3 is required for efficient erythropoiesis in this model system. (Blood. 2004;103:4310-4316)
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Blanc, C. Barres, P. Bette-Bobillo, and M. Vidal
Reticulocyte-secreted exosomes bind natural IgM antibodies: involvement of a ROS-activatable endosomal phospholipase iPLA2
Blood,
November 1, 2007;
110(9):
3407 - 3416.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. De Franceschi, L. Ronzoni, M. D. Cappellini, F. Cimmino, A. Siciliano, S. L. Alper, V. Servedio, C. Pozzobon, and A. Iolascon
K-CL co-transport plays an important role in normal and {beta} thalassemic erythropoiesis
Haematologica,
October 1, 2007;
92(10):
1319 - 1326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Rebe, S. Cathelin, S. Launay, R. Filomenko, L. Prevotat, C. L'Ollivier, E. Gyan, O. Micheau, S. Grant, A. Dubart-Kupperschmitt, et al.
Caspase-8 prevents sustained activation of NF-{kappa}B in monocytes undergoing macrophagic differentiation
Blood,
February 15, 2007;
109(4):
1442 - 1450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Zandy and S. Bassnett
Proteolytic Mechanisms Underlying Mitochondrial Degradation in the Ocular Lens
Invest. Ophthalmol. Vis. Sci.,
January 1, 2007;
48(1):
293 - 302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Q. Nhan, W. C. Liles, and S. M. Schwartz
Physiological Functions of Caspases Beyond Cell Death
Am. J. Pathol.,
September 1, 2006;
169(3):
729 - 737.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kalamvoki, U. Georgopoulou, and P. Mavromara
The NS5A Protein of the Hepatitis C Virus Genotype 1a Is Cleaved by Caspases to Produce C-terminal-truncated Forms of the Protein That Reside Mainly in the Cytosol
J. Biol. Chem.,
May 12, 2006;
281(19):
13449 - 13462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Martin, O. Sabido, P. Durand, and R. Levy
Phosphatidylserine externalization in human sperm induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction
Hum. Reprod.,
December 1, 2005;
20(12):
3459 - 3468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Palis
To condense is not to die
Blood,
September 15, 2005;
106(6):
1900 - 1900.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. W. Krauss, A. J. Lo, S. A. Short, M. J. Koury, N. Mohandas, and J. A. Chasis
Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins
Blood,
September 15, 2005;
106(6):
2200 - 2205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. F. Weber and A. S. Menko
The Canonical Intrinsic Mitochondrial Death Pathway Has a Non-apoptotic Role in Signaling Lens Cell Differentiation
J. Biol. Chem.,
June 10, 2005;
280(23):
22135 - 22145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Decordier, E. Cundari, and M. Kirsch-Volders
Influence of caspase activity on micronuclei detection: a possible role for caspase-3 in micronucleation
Mutagenesis,
May 1, 2005;
20(3):
173 - 179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Q. Nhan, W. C. Liles, and S. M. Schwartz
Role of Caspases in Death and Survival of the Plaque Macrophage
Arterioscler. Thromb. Vasc. Biol.,
May 1, 2005;
25(5):
895 - 903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Koury, S. T. Koury, P. Kopsombut, and M. C. Bondurant
In vitro maturation of nascent reticulocytes to erythrocytes
Blood,
March 1, 2005;
105(5):
2168 - 2174.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
