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Blood, 1 June 2004, Vol. 103, No. 11, pp. 4344-4352. Prepublished online as a Blood First Edition Paper on February 19, 2004; DOI 10.1182/blood-2003-07-2534. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-07-2534v1 103/11/4344    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, B. J. Articles by Chao, N. J. Search for Related Content PubMed PubMed Citation Articles by Chen, B. J. Articles by Chao, N. J. Related Collections Hematopoiesis and Stem Cells Immunobiology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation Benny J. Chen, Xiuyu Cui, Gregory D. Sempowski, Jos Domen, and Nelson J. Chao From the Bone Marrow Transplantation Program, Departments of Medicine and Immunology, Human Vaccine Institute, Duke University Medical Center, Durham, NC. In the current study, we tested whether higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation model (C57BL/Ka, CD45.1, Thy1.1C57BL/6, CD45.2, Thy1.2) using purified hematopoietic stem cells (c-Kit+Thy1.1lowLin-/lowSca-1+). There were 3 different doses of stem cells used (400, 1000, and 5000). Phenotypic analyses in peripheral blood and spleen demonstrated that higher numbers of infused stem cells are associated with more rapid regeneration of T cells (CD4+, CD8+, naive CD4+, naive CD8+) and B cells at early time points. The numbers of T and B cells eventually became equivalent between different dose groups at late time points. Production of interleukin-2 and inter-feron- per T cell was similar regardless of stem cell dose even when tested at the time when there were significant differences in peripheral T-cell counts. The improved immune recovery was attributed to a more rapid regeneration of donor-type immune cells. Higher numbers of total thymocytes and signal joint T-cell receptor excision circles were observed in the higher dose stem cell recipients, suggesting that accelerated regeneration of T cells was due to enhanced thymopoiesis. (Blood. 2004;103:4344-4352) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Mariotti, J. Foley, U. Jung, T. Borenstein, N. Kantardzic, S. Han, J. T. Hanson, E. Wong, N. Buxhoeveden, J. B. Trepel, et al. Ex Vivo Rapamycin Generates Apoptosis-Resistant Donor Th2 Cells That Persist In Vivo and Prevent Hemopoietic Stem Cell Graft Rejection J. Immunol., January 1, 2008; 180(1): 89 - 105. [Abstract] [Full Text] [PDF] M. H. Dallas, B. Varnum-Finney, P. J. Martin, and I. D. Bernstein Enhanced T-cell reconstitution by hematopoietic progenitors expanded ex vivo using the Notch ligand Delta1 Blood, April 15, 2007; 109(8): 3579 - 3587. [Abstract] [Full Text] [PDF] B. J. Chen, D. Deoliveira, X. Cui, N. T. Le, J. Son, J. F. Whitesides, and N. J. Chao Inability of memory T cells to induce graft-versus-host disease is a result of an abortive alloresponse Blood, April 1, 2007; 109(7): 3115 - 3123. [Abstract] [Full Text] [PDF] N. J. Chao, S. G. Emerson, and K. I. Weinberg Stem Cell Transplantation (Cord Blood Transplants) Hematology, January 1, 2004; 2004(1): 354 - 371. [Abstract] [Full Text] [PDF]

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