|
|
Blood, 15 January 2004, Vol. 103, No. 2, pp. 530-537.
Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-06-1797.
 Previous Article | Table of Contents | Next Article 
HEMATOPOIESIS
Identification of the earliest prethymic T-cell progenitors in murine fetal blood
Tomokatsu Ikawa,
Kyoko Masuda,
Min Lu,
Nagahiro Minato,
Yoshimoto Katsura, and
Hiroshi Kawamoto
From the Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan; Department of Immunology and Cell Biology, Graduate School of Biostudies, Kyoto University, Kyoto, Japan; Laboratory for Lymphocyte Development, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan; and Division of Cell Regeneration and Transplantation, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo, Japan.
During murine fetal development, hemato-poietic progenitors start to colonize the thymic anlage at day 11 of gestation via blood stream. The present study aims at identifying the earliest prethymic progenitors in circulation. Here, we show that the interleukin-7 receptor–positive (IL-7R+) cells in Lin– c-kit+ population are circulating exclusively between days 11 and 14 of fetal age. Clonal analysis revealed that these IL-7R+ cells mostly contain T-cell lineage–restricted progenitors (p-Ts). The proportion of circulating p-Ts reaches 30% of the total p-Ts during these fetal ages, whereas virtually all B-cell lineage–restricted progenitors stay in the fetal liver, suggesting that the p-Ts are selectively released to the circulation. The circulating p-Ts retain the potential to generate natural killer cells and dendritic cells and exhibit extensive proliferation before the occurrence of T-cell receptor  (TCR) chain gene rearrangement. We propose that the wave of p-Ts in fetal blood disclosed by this study represents the ontogenically earliest thymic immigrants.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. de Andres, I. Cortegano, N. Serrano, B. del Rio, P. Martin, P. Gonzalo, M. A. R. Marcos, and M. L. Gaspar
A Population of CD19highCD45R /lowCD21low B Lymphocytes Poised for Spontaneous Secretion of IgG and IgA Antibodies
J. Immunol.,
October 15, 2007;
179(8):
5326 - 5334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Masuda, K. Kakugawa, T. Nakayama, N. Minato, Y. Katsura, and H. Kawamoto
T Cell Lineage Determination Precedes the Initiation of TCRbeta Gene Rearrangement
J. Immunol.,
September 15, 2007;
179(6):
3699 - 3706.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Heinzel, C. Benz, V. C. Martins, I. D. Haidl, and C. C. Bleul
Bone Marrow-Derived Hemopoietic Precursors Commit to the T Cell Lineage Only after Arrival in the Thymic Microenvironment
J. Immunol.,
January 15, 2007;
178(2):
858 - 868.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Perry, R. S. Welner, T. Kouro, P. W. Kincade, and X.-H. Sun
Primitive lymphoid progenitors in bone marrow with T lineage reconstituting potential.
J. Immunol.,
September 1, 2006;
177(5):
2880 - 2887.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ikawa, H. Kawamoto, A. W. Goldrath, and C. Murre
E proteins and Notch signaling cooperate to promote T cell lineage specification and commitment
J. Exp. Med.,
May 15, 2006;
203(5):
1329 - 1342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yokota, J. Huang, M. Tavian, Y. Nagai, J. Hirose, J.-C. Zuniga-Pflucker, B. Peault, and P. W. Kincade
Tracing the first waves of lymphopoiesis in mice
Development,
May 15, 2006;
133(10):
2041 - 2051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. L. Veinotte, C. P. Greenwood, N. Mohammadi, C. A. Parachoniak, and F. Takei
Expression of rearranged TCR{gamma} genes in natural killer cells suggests a minor thymus-dependent pathway of lineage commitment
Blood,
April 1, 2006;
107(7):
2673 - 2679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lu, R. Tayu, T. Ikawa, K. Masuda, I. Matsumoto, H. Mugishima, H. Kawamoto, and Y. Katsura
The Earliest Thymic Progenitors in Adults Are Restricted to T, NK, and Dendritic Cell Lineage and Have a Potential to Form More Diverse TCR{beta} Chains than Fetal Progenitors
J. Immunol.,
November 1, 2005;
175(9):
5848 - 5856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. C. Harman, W. E. Jenkinson, S. M. Parnell, S. W. Rossi, E. J. Jenkinson, and G. Anderson
T/B lineage choice occurs prior to intrathymic Notch signaling
Blood,
August 1, 2005;
106(3):
886 - 892.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. T. Petrie and P. W. Kincade
Many roads, one destination for T cell progenitors
J. Exp. Med.,
July 5, 2005;
202(1):
11 - 13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Miyazaki, H. Kawamoto, Y. Kato, M. Itoi, K. Miyazaki, K. Masuda, S. Tashiro, H. Ishihara, K. Igarashi, T. Amagai, et al.
Polycomb Group Gene mel-18 Regulates Early T Progenitor Expansion by Maintaining the Expression of Hes-1, a Target of the Notch Pathway
J. Immunol.,
March 1, 2005;
174(5):
2507 - 2516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Masuda, M. Itoi, T. Amagai, N. Minato, Y. Katsura, and H. Kawamoto
Thymic Anlage Is Colonized by Progenitors Restricted to T, NK, and Dendritic Cell Lineages
J. Immunol.,
March 1, 2005;
174(5):
2525 - 2532.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zamisch, B. Moore-Scott, D.-m. Su, P. J. Lucas, N. Manley, and E. R. Richie
Ontogeny and Regulation of IL-7-Expressing Thymic Epithelial Cells
J. Immunol.,
January 1, 2005;
174(1):
60 - 67.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Liu, T. Ueno, S. Kuse, F. Saito, T. Nitta, L. Piali, H. Nakano, T. Kakiuchi, M. Lipp, G. A. Hollander, et al.
The role of CCL21 in recruitment of T-precursor cells to fetal thymi
Blood,
January 1, 2005;
105(1):
31 - 39.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Schmitt, M. Ciofani, H. T. Petrie, and J. C. Zuniga-Pflucker
Maintenance of T Cell Specification and Differentiation Requires Recurrent Notch Receptor-Ligand Interactions
J. Exp. Med.,
August 16, 2004;
200(4):
469 - 479.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
