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Blood, 1 February 2004, Vol. 103, No. 3, pp. 1099-1104. Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-04-1069. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-04-1069v1 103/3/1099    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, C. Articles by Hänsch, G. M. Search for Related Content PubMed PubMed Citation Articles by Wagner, C. Articles by Hänsch, G. M. Related Collections Immunobiology Phagocytes Apoptosis Gene Expression Related Letters in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES Granzyme B and perforin: constitutive expression in human polymorphonuclear neutrophils Christof Wagner, Christof Iking-Konert, Birgit Denefleh, Sabine Stegmaier, Friederike Hug, and G. Maria Hänsch From the Institut für Immunologie der Universität Heidelberg, Germany. Polymorphonuclear neutrophils (PMNs) produce an abundance of bactericidal and cytotoxic molecules consistent with their role as first-line defense against bacterial infection. PMNs, however, also cause efficient cellular cytotoxicity when targeted through Fc receptors to appropriate antibody-coated target cells. Although this so-called antibody-dependent cellular cytotoxicity (ADCC) was described many years ago, the mechanism of killing is still elusive. We now have found that PMNs contain perforin and granzyme B, the 2 molecules known as the cytotoxic entity of natural killer cells and of cytotoxic T lymphocytes as well. Lysates of PMNs were lytic for chicken erythrocytes in a time-, temperature-, and Ca2+-dependent manner. Moreover, apoptosis of Jurkat cells was induced, consistent with the observation that the PMN lysates contain enzymatically active granzyme B. Taken together, our data provide evidence for the presence of perforin and granzyme B within the cytotoxic arsenal of PMNs. (Blood. 2004;103:1099-1104) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letters in Blood Online: Granzyme A: an additional weapon of human polymorphonuclear neutrophils (PMNs) in innate immunity? Kathrin Hochegger, Philipp Eller, and Alexander R. Rosenkranz Blood 2004 103: 1176. [Full Text] [PDF] Human neutrophils lack granzyme A, granzyme B, and perforin Sunil S. Metkar and Christopher J. Froelich Blood 2004 104: 905-906. [Full Text] [PDF] This article has been cited by other articles: M. Wellington, K. Dolan, and D. J. Krysan Live Candida albicans Suppresses Production of Reactive Oxygen Species in Phagocytes Infect. Immun., January 1, 2009; 77(1): 405 - 413. [Abstract] [Full Text] [PDF] H. Horner, C. Frank, C. Dechant, R. Repp, M. Glennie, M. Herrmann, and B. Stockmeyer Intimate Cell Conjugate Formation and Exchange of Membrane Lipids Precede Apoptosis Induction in Target Cells during Antibody-Dependent, Granulocyte-Mediated Cytotoxicity J. Immunol., July 1, 2007; 179(1): 337 - 345. [Abstract] [Full Text] [PDF] S. Jaillon, G. Peri, Y. Delneste, I. Fremaux, A. Doni, F. Moalli, C. Garlanda, L. Romani, H. Gascan, S. Bellocchio, et al. The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps J. Exp. Med., April 16, 2007; 204(4): 793 - 804. [Abstract] [Full Text] [PDF] C. D. Coldren, J. A. Nick, K. R. Poch, M. D. Woolum, B. W. Fouty, J. M. O'Brien, M. P. Gruber, M. R. Zamora, D. Svetkauskaite, D. A. Richter, et al. Functional and genomic changes induced by alveolar transmigration in human neutrophils Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1267 - L1276. [Abstract] [Full Text] [PDF] C. M. Tschopp, N. Spiegl, S. Didichenko, W. Lutmann, P. Julius, J. C. Virchow, C. E. Hack, and C. A. Dahinden Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma Blood, October 1, 2006; 108(7): 2290 - 2299. [Abstract] [Full Text] [PDF] B. Bade, H. E. Boettcher, J. Lohrmann, C. Hink-Schauer, K. Bratke, D. E. Jenne, J. C. Virchow Jr, and W. Luttmann Differential expression of the granzymes A, K and M and perforin in human peripheral blood lymphocytes Int. Immunol., November 1, 2005; 17(11): 1419 - 1428. [Abstract] [Full Text] [PDF] P. Martin, R. Wallich, J. Pardo, A. Mullbacher, M. Munder, M. Modolell, and M. M. Simon Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin: similarities or differences with human polymorphonuclear leukocytes? Blood, October 15, 2005; 106(8): 2871 - 2878. [Abstract] [Full Text] [PDF] J. C. Choy, V. H.Y. Hung, A. L. Hunter, P. K. Cheung, B. Motyka, I. S. Goping, T. Sawchuk, R. C. Bleackley, T. J. Podor, B. M. McManus, et al. Granzyme B Induces Smooth Muscle Cell Apoptosis in the Absence of Perforin: Involvement of Extracellular Matrix Degradation Arterioscler Thromb Vasc Biol, December 1, 2004; 24(12): 2245 - 2250. [Abstract] [Full Text] [PDF] K. A. Lindstedt, M. J. Leskinen, and P. T. Kovanen Proteolysis of the Pericellular Matrix: A Novel Element Determining Cell Survival and Death in the Pathogenesis of Plaque Erosion and Rupture Arterioscler Thromb Vasc Biol, August 1, 2004; 24(8): 1350 - 1358. [Abstract] [Full Text] [PDF] S. S. Metkar and C. J. Froelich Human neutrophils lack granzyme A, granzyme B, and perforin Blood, August 1, 2004; 104(3): 905 - 906. [Full Text] [PDF] W. J. Grossman, T. J. Ley, K. Hochegger, C. Wagner, A. Rosenkranz, and G. M. Hansch Granzymes A and B are not expressed in human neutrophils Blood, August 1, 2004; 104(3): 906 - 908. [Full Text] [PDF]

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