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Blood, 15 February 2004, Vol. 103, No. 4, pp. 1270-1277. Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-05-1618. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-05-1618v1 103/4/1270    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sergejeva, S. Articles by Lötvall, J. Search for Related Content PubMed PubMed Citation Articles by Sergejeva, S. Articles by Lötvall, J. Related Collections Hematopoiesis and Stem Cells Immunobiology Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Allergen exposure–induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments Svetlana Sergejeva, Anna-Karin Johansson, Carina Malmhäll, and Jan Lötvall From The Lung Pharmacology Group, Department of Respiratory Medicine and Allergology, Institute of Internal Medicine, Göteborg University, Göteborg, Sweden We hypothesized that the allergen-induced increased number of airway eosinophils results from increased recruitment of eosinophils from bone marrow (BM) and local development of CD34+ cells into eosinophils. We also assumed that the phenotype of airway eosinophils depends on whether these cells have differentiated within BM or airway. C57BL/6 mice were sensitized and subsequently exposed to ovalbumin (OVA) on 5 consecutive days. Newly produced cells were labeled with a thymidine analog. Clonogenic activity and interleukin 5 (IL-5) release from bronchoalveolar lavage fluid (BALf) CD34+ cells were evaluated by using cell-culture techniques. Allergen exposure induces increase in CD135+ primitive myeloid progenitors within the BM CD34+ cell population, without significant changes in total number of CD34+ cells or newly produced CD34+ cells. CD34+/IL-5R+ cells in the first stage of cell differentiation were found only in BM, arguing that early commitment of CD34+ cells into the eosinophil lineage is restricted to the BM compartment. Allergen exposure induces a shift in differentiation of BM, blood, and BALf eosinophillineage–committed CD34+ cells toward mature eosinophils and recruitment of these cells via blood into airway. We further demonstrate in vitro that ability to multiply persists in BALf CD34+ cells but not CD34– cells, likely via autocrine IL-5 release and IL-5–induced up-regulation of IL-5R. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. H. Kang, D. H. Lee, H. Seo, J. S. Park, K. H. Nam, S. Y. Shin, C.-S. Park, and I. Y. Chung Regulation of Functional Phenotypes of Cord Blood Derived Eosinophils by {gamma}-Secretase Inhibitor Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 571 - 577. [Abstract] [Full Text] [PDF]

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