|
|
Blood, 15 February 2004, Vol. 103, No. 4, pp. 1278-1285.
Prepublished online as a Blood First Edition Paper on October 23, 2003; DOI 10.1182/blood-2003-06-2158.
 Previous Article | Table of Contents | Next Article 
HEMATOPOIESIS
BUBR1 deficiency results in abnormal megakaryopoiesis
Qi Wang,
Tongyi Liu,
Yuqiang Fang,
Suqing Xie,
Xuan Huang,
Radma Mahmood,
Gita Ramaswamy,
Kathleen M. Sakamoto,
Zbigniew Darzynkiewicz,
Ming Xu, and
Wei Dai
From the Division of Molecular Carcinogenesis, Department of Medicine, Brander Cancer Institute, and Department of Pathology, New York Medical College, Valhalla, NY; Core Facility for Histopathology, Albert Einstein College of Medicine, Bronx, NY; Division of Hematology-Oncology, Mattel Children's Hospital, Department of Pathology and Laboratory Medicine, University of California at Los Angeles (UCLA) Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, Los Angeles, CA; and Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH.
The physiologic function of BUBR1, a key component of the spindle checkpoint, was examined by generating BUBR1-mutant mice. BUBR1–/– embryos failed to survive beyond day 8.5 in utero as a result of extensive apoptosis. Whereas BUBR1+/– blastocysts grew relatively normally in vitro, BUBR1–/– blastocysts exhibited impaired proliferation and atrophied. Adult BUBR1+/– mice manifested splenomegaly and abnormal megakaryopoiesis. BUBR1 haploinsufficiency resulted in an increase in the number of splenic megakaryocytes, which was correlated with an increase in megakaryocytic, but a decrease in erythroid, progenitors in bone marrow cells. RNA interference–mediated down-regulation of BUBR1 also caused an increase in polyploidy formation in murine embryonic fibroblast cells and enhanced megakaryopoiesis in bone marrow progenitor cells. However, enhanced megakaryopoiesis in BUBR1+/– mice was not correlated with a significant increase in platelets in peripheral blood, which was at least partly due to a defect in the formation of proplatelet-producing megakaryocytes. Together, these results indicate that BUBR1 is essential for early embryonic development and normal hematopoiesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. A.A. Weaver and D. W. Cleveland
Aneuploidy: Instigator and Inhibitor of Tumorigenesis
Cancer Res.,
November 1, 2007;
67(21):
10103 - 10105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Jeganathan, L. Malureanu, D. J. Baker, S. C. Abraham, and J. M. van Deursen
Bub1 mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis
J. Cell Biol.,
October 22, 2007;
179(2):
255 - 267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Leung, Y. Xu, B. Mularski, H. Liu, S. Gurbuxani, and J. D. Crispino
Requirements for survivin in terminal differentiation of erythroid cells and maintenance of hematopoietic stem and progenitor cells
J. Exp. Med.,
July 9, 2007;
204(7):
1603 - 1611.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Perez de Castro, G. de Carcer, and M. Malumbres
A census of mitotic cancer genes: new insights into tumor cell biology and cancer therapy
Carcinogenesis,
May 1, 2007;
28(5):
899 - 912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-L. Lo, J.-C. Yu, S.-T. Chen, G.-C. Hsu, Y.-C. Mau, S.-L. Yang, P.-E. Wu, and C.-Y. Shen
Breast cancer risk associated with genotypic polymorphism of the mitotic checkpoint genes: a multigenic study on cancer susceptibility
Carcinogenesis,
May 1, 2007;
28(5):
1079 - 1086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ito, C. R. Mantel, M.-K. Han, S. Basu, S. Fukuda, S. Cooper, and H. E. Broxmeyer
Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells
Blood,
March 1, 2007;
109(5):
1923 - 1930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Lee, D. J. Lee, S. P. Oh, H. D. Park, H. H. Nam, J. M. Kim, and D.-S. Lim
Mouse emi1 Has an Essential Function in Mitotic Progression during Early Embryogenesis.
Mol. Cell. Biol.,
July 1, 2006;
26(14):
5373 - 5381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Dai and X. Wang
Aging in check.
Sci. Aging Knowl. Environ.,
March 15, 2006;
2006(7):
pe9 - pe9.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. J. Baker, K. B. Jeganathan, L. Malureanu, C. Perez-Terzic, A. Terzic, and J. M.A. van Deursen
Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice.
J. Cell Biol.,
February 13, 2006;
172(4):
529 - 540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kim, K. Murphy, F. Liu, S. E. Parker, M. L. Dowling, W. Baff, and G. D. Kao
Caspase-Mediated Specific Cleavage of BubR1 Is a Determinant of Mitotic Progression
Mol. Cell. Biol.,
November 1, 2005;
25(21):
9232 - 9248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Huang, R. Hatcher, J. P. York, and P. Zhang
Securin and Separase Phosphorylation Act Redundantly to Maintain Sister Chromatid Cohesion in Mammalian Cells
Mol. Biol. Cell,
October 1, 2005;
16(10):
4725 - 4732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gurbuxani, Y. Xu, G. Keerthivasan, A. Wickrema, and J. D. Crispino
Differential requirements for survivin in hematopoietic cell development
PNAS,
August 9, 2005;
102(32):
11480 - 11485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. V. Rao, Y.-M. Yang, M. V. Swamy, T. Liu, Y. Fang, R. Mahmood, M. Jhanwar-Uniyal, and W. Dai
Colonic tumorigenesis in BubR1+/-ApcMin/+ compound mutant mice is linked to premature separation of sister chromatids and enhanced genomic instability
PNAS,
March 22, 2005;
102(12):
4365 - 4370.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Myung, S. Smith, and R. D. Kolodner
Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae
PNAS,
November 9, 2004;
101(45):
15980 - 15985.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Geddis and K. Kaushansky
Megakaryocytes express functional Aurora-B kinase in endomitosis
Blood,
August 15, 2004;
104(4):
1017 - 1024.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
