SEARCH:   Advanced

Blood, 15 February 2004, Vol. 103, No. 4, pp. 1408-1416. Prepublished online as a Blood First Edition Paper on October 16, 2003; DOI 10.1182/blood-2003-03-0930. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-03-0930v1 103/4/1408    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Landais, E. Articles by Houssaint, E. Search for Related Content PubMed PubMed Citation Articles by Landais, E. Articles by Houssaint, E. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Direct killing of Epstein-Barr virus (EBV)–infected B cells by CD4 T cells directed against the EBV lytic protein BHRF1 Elise Landais, Xavier Saulquin, Emmanuel Scotet, Lydie Trautmann, Marie-Alix Peyrat, John L. Yates, William W. Kwok, Marc Bonneville, and Elisabeth Houssaint From the INSERM U463, Institut de Biologie, Nantes, France; Faculté des Sciences de Nantes, Nantes, France; Virginia Mason Research Center, Seattle, WA; and Roswell Park Center Institute, Buffalo, NY. Due to their low frequency, CD4 T-cell responses to Epstein-Barr virus (EBV) lytic antigens are, so far, poorly characterized. Human peptide major histocompatibility complex (MHC) class II multimers provide a means to detect and characterize such rare T cells. Along a screening of T-cell responses to lytic or latent EBV antigens within peripheral blood leukocyte (PBL)– or synovial-derived CD4 T-cell lines, we identified an human leukocyte antigen–DR*0401 (HLA-DR*0401)–restricted epitope derived from BHRF1 (BamHI fragment H rightward open reading frame 1), a viral protein produced during the early stages of the lytic cycle. We show here that T-cell responses to this particular BHRF1 epitope are shared by most EBV-infected DR*0401+ individuals, as BHRF1-specific CD4 T cells could be sorted out from all the DRB*0401 T-cell lines analyzed, using magnetic beads coated with recombinant BHRF1/DR*0401 complexes. Sorting with these peptide MHC class II multimers was very efficient, as the yield of recovery of BHRF1-specific T cells was nearly 100%. Functional analysis of a large number of clones responding to BHRF1/DR*0401 demonstrated their cytolytic action against autologous and allogeneic DR*0401+ EBV-transformed B-lymphoblastoid cell lines (B-LCLs), with 40% to 80% killing efficiency and potent interferon production, thus suggesting that this CD4 T-cell population contributes to the control of EBV replication. B-LCL lysis by these T-cell clones was DR*0401 dependent, EBV dependent, and was not merely due to bystander killing. Taken together, these data provide the first demonstration that a lytic antigen can induce a direct cytolytic response against EBV-infected cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. A. Stuller and E. Flano CD4 T Cells Mediate Killing during Persistent Gammaherpesvirus 68 Infection J. Virol., May 1, 2009; 83(9): 4700 - 4703. [Abstract] [Full Text] [PDF] J. Klibi, T. Niki, A. Riedel, C. Pioche-Durieu, S. Souquere, E. Rubinstein, S. L. E. Moulec, J. Guigay, M. Hirashima, F. Guemira, et al. Blood diffusion and Th1-suppressive effects of galectin-9-containing exosomes released by Epstein-Barr virus-infected nasopharyngeal carcinoma cells Blood, February 26, 2009; 113(9): 1957 - 1966. [Abstract] [Full Text] [PDF] J. D. Lunemann, O. Frey, T. Eidner, M. Baier, S. Roberts, J. Sashihara, R. Volkmer, J. I. Cohen, G. Hein, T. Kamradt, et al. Increased Frequency of EBV-Specific Effector Memory CD8+ T Cells Correlates with Higher Viral Load in Rheumatoid Arthritis J. Immunol., July 15, 2008; 181(2): 991 - 1000. [Abstract] [Full Text] [PDF] S. Kannanganat, C. Ibegbu, L. Chennareddi, H. L. Robinson, and R. R. Amara Multiple-Cytokine-Producing Antiviral CD4 T Cells Are Functionally Superior to Single-Cytokine-Producing Cells J. Virol., August 15, 2007; 81(16): 8468 - 8476. [Abstract] [Full Text] [PDF] G. J. MacArthur, A. D. Wilson, M. A. Birchall, and A. J. Morgan Primary CD4+ T-Cell Responses Provide both Helper and Cytotoxic Functions during Epstein-Barr Virus Infection and Transformation of Fetal Cord Blood B Cells J. Virol., May 1, 2007; 81(9): 4766 - 4775. [Abstract] [Full Text] [PDF] G. S. Taylor, H. M. Long, T. A. Haigh, M. Larsen, J. Brooks, and A. B. Rickinson A Role for Intercellular Antigen Transfer in the Recognition of EBV-Transformed B Cell Lines by EBV Nuclear Antigen-Specific CD4+ T Cells J. Immunol., September 15, 2006; 177(6): 3746 - 3756. [Abstract] [Full Text] [PDF] E. Landais, A. Morice, H. M. Long, T. A. Haigh, B. Charreau, M. Bonneville, G. S. Taylor, and E. Houssaint EBV-Specific CD4+ T Cell Clones Exhibit Vigorous Allogeneic Responses J. Immunol., August 1, 2006; 177(3): 1427 - 1433. [Abstract] [Full Text] [PDF] K. N. Heller, C. Gurer, and C. Munz Virus-specific CD4+ T cells: ready for direct attack J. Exp. Med., April 17, 2006; 203(4): 805 - 808. [Abstract] [Full Text] [PDF] E. Landais, X. Saulquin, M. Bonneville, and E. Houssaint Long-Term MHC Class II Presentation of the EBV Lytic Protein BHRF1 by EBV Latently Infected B Cells following Capture of BHRF1 Antigen J. Immunol., December 15, 2005; 175(12): 7939 - 7946. [Abstract] [Full Text] [PDF] E. Piriou, K. van Dort, N. M. Nanlohy, M. H. J. van Oers, F. Miedema, and D. van Baarle Loss of EBNA1-specific memory CD4+ and CD8+ T cells in HIV-infected patients progressing to AIDS-related non-Hodgkin lymphoma Blood, November 1, 2005; 106(9): 3166 - 3174. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020