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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1669-1675. Prepublished online as a Blood First Edition Paper on October 23, 2003; DOI 10.1182/blood-2003-05-1670. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-05-1670v1 103/5/1669    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lee, O. K. Articles by Chen, T.-H. Search for Related Content PubMed PubMed Citation Articles by Lee, O. K. Articles by Chen, T.-H. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Isolation of multipotent mesenchymal stem cells from umbilical cord blood Oscar K. Lee, Tom K. Kuo, Wei-Ming Chen, Kuan-Der Lee, Shie-Liang Hsieh, and Tain-Hsiung Chen From the Department of Orthopaedics and Traumatology, Veterans General Hospital-Taipei, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan; Cancer Research Division, National Health Research Institute, Taipei, Taiwan; and Department of Immunology, National Yang-Ming University, Taipei, Taiwan. It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different connective tissue lineages such as bone, cartilage, and fat, and little success has been reported in the literature about the isolation of such cells from cord blood. Here we report a novel method to obtain single cell-derived, clonally expanded mesenchymal stem cells that are of multilineage differentiation potential by negative immunoselection and limiting dilution. The immunophenotype of these clonally expanded cells is consistent with that reported for bone marrow mesenchymal stem cells. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, and fat. Surprisingly, these cells were also able to differentiate into neuroglial- and hepatocyte-like cells under appropriate induction conditions and, thus, these cells may be more than mesenchymal stem cells as evidenced by their ability to differentiate into cell types of all 3 germ layers. In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D.-H. Lee, Y. Ahn, S. U. Kim, K.-C. Wang, B.-K. Cho, J. H. Phi, I. H. Park, P. M. Black, R. S. Carroll, J. Lee, et al. Targeting Rat Brainstem Glioma Using Human Neural Stem Cells and Human Mesenchymal Stem Cells Clin. Cancer Res., August 1, 2009; 15(15): 4925 - 4934. [Abstract] [Full Text] [PDF] V. Katsares, Z. Paparidis, E. Nikolaidou, I. Karvounidou, K.-A. Ardelean, N. Drossas, N. Grigoriadis, and J. Grigoriadis Reference Ranges for Umbilical Cord Blood Hematological Values Lab Med, July 1, 2009; 40(7): 437 - 439. [Abstract] [Full Text] [PDF] T. Mizokami, H. Hisha, S. Okazaki, T. Takaki, X.-L. Wang, C.-Y. Song, Q. Li, J. Kato, N. Hosaka, M. Inaba, et al. Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells Haematologica, May 1, 2009; 94(5): 618 - 628. [Abstract] [Full Text] [PDF] H Aurich, M Sgodda, P Kaltwasser, M Vetter, A Weise, T Liehr, M Brulport, J G Hengstler, M M Dollinger, W E Fleig, et al. Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo Gut, April 1, 2009; 58(4): 570 - 581. [Abstract] [Full Text] [PDF] S. M. Kim, J. Y. Lim, S. I. Park, C. H. Jeong, J. H. Oh, M. Jeong, W. Oh, S.-H. Park, Y.-C. Sung, and S.-S. 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Functional integration of hepatocytes derived from human mesenchymal stem cells into mouse livers Gut, March 1, 2007; 56(3): 405 - 415. [Abstract] [Full Text] [PDF] K. W. Christopherson, T. Bakhshi, S. Bodie, S. Kidd, R. Zabriskie, and S. Ramin Mesenchymal Stem Cells from the Wharton's Jelly of Umbilical Cord Segments Support the Maintenance of Long Term Culture-Initiating Cells from Cord Blood. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 3650 - 3650. [Abstract] [PDF] K. H. Wu, Y. L. Liu, B. Zhou, and Z. C. Han Cellular therapy and myocardial tissue engineering: the role of adult stem and progenitor cells Eur. J. Cardiothorac. Surg., November 1, 2006; 30(5): 770 - 781. [Abstract] [Full Text] [PDF] T. Yazawa, T. Mizutani, K. Yamada, H. Kawata, T. Sekiguchi, M. Yoshino, T. Kajitani, Z. Shou, A. Umezawa, and K. 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Cell, March 1, 2005; 16(3): 1491 - 1499. [Abstract] [Full Text] [PDF]

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