|
|
Blood, 1 March 2004, Vol. 103, No. 5, pp. 1710-1719.
Prepublished online as a Blood First Edition Paper on October 30, 2003; DOI 10.1182/blood-2003-06-1783.
 Previous Article | Table of Contents | Next Article 
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Syndecan-2 is essential for angiogenic sprouting during zebrafish development
Eleanor Chen,
Spencer Hermanson, and
Stephen C. Ekker
From the Arnold and Mabel Beckman Center for Transposon Research, the Department of Genetics, Cell Biology, and Development, University of Minnesota; the Molecular, Cellular, Developmental Biology, and Genetics Graduate Program, University of Minnesota; and the Combined MD/PhD training program, University of Minnesota, Minneapolis, MN.
We used a morpholino-based gene-targeting screen to identify a novel protein essential for vascular development using the zebrafish, Danio rerio. We show that syndecan-2, a cell-surface heparan sulfate proteoglycan, is essential for angiogenic sprouting during embryogenesis. The vascular function of syndecan-2 is likely conserved, as zebrafish and mouse syndecan-2 show similar expression patterns around major trunk vessels, and human syndecan-2 can restore angiogenic sprouting in syndecan-2 morphants. In contrast, forced expression of a truncated form of syndecan-2 results in embryos with defects in angiogenesis, indicating that the highly conserved cytoplasmic tail is important for the vascular function of syndecan-2. We further show that vascular endothelial growth factor (VEGF) and syndecan-2 genetically interact in vivo using both gain-of-function and loss-of-function studies in zebrafish. VEGF-mediated ectopic signaling is compromised in syndecan-2 morphants, and ectopic syndecan-2 potentiates ectopic VEGF signaling. Syndecan-2 as a novel angiogenic factor is a potential candidate for use in the development of angiogenesis-based therapies.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. B. Arrington and H. J. Yost
Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo
Development,
September 15, 2009;
136(18):
3143 - 3152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Takenaka, M. Horiba, H. Ishiguro, A. Sumida, M. Hojo, A. Usui, T. Akita, S. Sakuma, Y. Ueda, I. Kodama, et al.
Midkine prevents ventricular remodeling and improves long-term survival after myocardial infarction
Am J Physiol Heart Circ Physiol,
February 1, 2009;
296(2):
H462 - H469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Whiteford, S. Ko, W. Lee, and J. R. Couchman
Structural and Cell Adhesion Properties of Zebrafish Syndecan-4 Are Shared with Higher Vertebrates
J. Biol. Chem.,
October 24, 2008;
283(43):
29322 - 29330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Patan
Lycat and cloche at the Switch Between Blood Vessel Growth and Differentiation?
Circ. Res.,
May 9, 2008;
102(9):
1005 - 1007.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A M Patterson, A Cartwright, G David, O Fitzgerald, B Bresnihan, B A Ashton, and J Middleton
Differential expression of syndecans and glypicans in chronically inflamed synovium
Ann Rheum Dis,
May 1, 2008;
67(5):
592 - 601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. C. Dews and K. R. MacKenzie
Transmembrane domains of the syndecan family of growth factor coreceptors display a hierarchy of homotypic and heterotypic interactions
PNAS,
December 26, 2007;
104(52):
20782 - 20787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Kim, I-H. Liu, Y. Song, J.-A. Lee, W. Halfter, R. J. Balice-Gordon, E. Linney, and G. J. Cole
Agrin is required for posterior development and motor axon outgrowth and branching in embryonic zebrafish
Glycobiology,
February 1, 2007;
17(2):
231 - 247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. V. Byzova
Matrix rules: microfibrillar protein controls vascular development
Blood,
June 1, 2006;
107(11):
4202 - 4203.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Chen, J. D. Larson, and S. C. Ekker
Functional analysis of zebrafish microfibril-associated glycoprotein-1 (Magp1) in vivo reveals roles for microfibrils in vascular development and function
Blood,
June 1, 2006;
107(11):
4364 - 4374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Y. Fears, C. L. Gladson, and A. Woods
Syndecan-2 Is Expressed in the Microvasculature of Gliomas and Regulates Angiogenic Processes in Microvascular Endothelial Cells
J. Biol. Chem.,
May 26, 2006;
281(21):
14533 - 14536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Miano
Fishing for Function in Zebrafish
Circ. Res.,
March 31, 2006;
98(6):
723 - 726.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J.M. Best, J. W. Gillespie, Y. Yi, G. V.R. Chandramouli, M. A. Perlmutter, Y. Gathright, H. S. Erickson, L. Georgevich, M. A. Tangrea, P. H. Duray, et al.
Molecular Alterations in Primary Prostate Cancer after Androgen Ablation Therapy
Clin. Cancer Res.,
October 1, 2005;
11(19):
6823 - 6834.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kubota, Y. Oike, S. Satoh, Y. Tabata, Y. Niikura, T. Morisada, M. Akao, T. Urano, Y. Ito, T. Miyamoto, et al.
Cooperative interaction of Angiopoietin-like proteins 1 and 2 in zebrafish vascular development
PNAS,
September 20, 2005;
102(38):
13502 - 13507.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ashikari-Hada, H. Habuchi, Y. Kariya, and K. Kimata
Heparin Regulates Vascular Endothelial Growth Factor165-dependent Mitogenic Activity, Tube Formation, and Its Receptor Phosphorylation of Human Endothelial Cells: COMPARISON OF THE EFFECTS OF HEPARIN AND MODIFIED HEPARINS
J. Biol. Chem.,
September 9, 2005;
280(36):
31508 - 31515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Tkachenko, J. M. Rhodes, and M. Simons
Syndecans: New Kids on the Signaling Block
Circ. Res.,
March 18, 2005;
96(5):
488 - 500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
