SEARCH:   Advanced

Blood, 1 March 2004, Vol. 103, No. 5, pp. 1710-1719. Prepublished online as a Blood First Edition Paper on October 30, 2003; DOI 10.1182/blood-2003-06-1783. This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 2003-06-1783v1 103/5/1710    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, E. Articles by Ekker, S. C. Search for Related Content PubMed PubMed Citation Articles by Chen, E. Articles by Ekker, S. C. Related Collections Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Syndecan-2 is essential for angiogenic sprouting during zebrafish development Eleanor Chen, Spencer Hermanson, and Stephen C. Ekker From the Arnold and Mabel Beckman Center for Transposon Research, the Department of Genetics, Cell Biology, and Development, University of Minnesota; the Molecular, Cellular, Developmental Biology, and Genetics Graduate Program, University of Minnesota; and the Combined MD/PhD training program, University of Minnesota, Minneapolis, MN. We used a morpholino-based gene-targeting screen to identify a novel protein essential for vascular development using the zebrafish, Danio rerio. We show that syndecan-2, a cell-surface heparan sulfate proteoglycan, is essential for angiogenic sprouting during embryogenesis. The vascular function of syndecan-2 is likely conserved, as zebrafish and mouse syndecan-2 show similar expression patterns around major trunk vessels, and human syndecan-2 can restore angiogenic sprouting in syndecan-2 morphants. In contrast, forced expression of a truncated form of syndecan-2 results in embryos with defects in angiogenesis, indicating that the highly conserved cytoplasmic tail is important for the vascular function of syndecan-2. We further show that vascular endothelial growth factor (VEGF) and syndecan-2 genetically interact in vivo using both gain-of-function and loss-of-function studies in zebrafish. VEGF-mediated ectopic signaling is compromised in syndecan-2 morphants, and ectopic syndecan-2 potentiates ectopic VEGF signaling. Syndecan-2 as a novel angiogenic factor is a potential candidate for use in the development of angiogenesis-based therapies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Takenaka, M. Horiba, H. Ishiguro, A. Sumida, M. Hojo, A. Usui, T. Akita, S. Sakuma, Y. Ueda, I. Kodama, et al. Midkine prevents ventricular remodeling and improves long-term survival after myocardial infarction Am J Physiol Heart Circ Physiol, February 1, 2009; 296(2): H462 - H469. [Abstract] [Full Text] [PDF] S. Patan Lycat and cloche at the Switch Between Blood Vessel Growth and Differentiation? Circ. Res., May 9, 2008; 102(9): 1005 - 1007. [Full Text] [PDF] A M Patterson, A Cartwright, G David, O Fitzgerald, B Bresnihan, B A Ashton, and J Middleton Differential expression of syndecans and glypicans in chronically inflamed synovium Ann Rheum Dis, May 1, 2008; 67(5): 592 - 601. [Abstract] [Full Text] [PDF] I. C. Dews and K. R. MacKenzie Transmembrane domains of the syndecan family of growth factor coreceptors display a hierarchy of homotypic and heterotypic interactions PNAS, December 26, 2007; 104(52): 20782 - 20787. [Abstract] [Full Text] [PDF] M. J. Kim, I-H. Liu, Y. Song, J.-A. Lee, W. Halfter, R. J. Balice-Gordon, E. Linney, and G. J. Cole Agrin is required for posterior development and motor axon outgrowth and branching in embryonic zebrafish Glycobiology, February 1, 2007; 17(2): 231 - 247. [Abstract] [Full Text] [PDF] T. V. Byzova Matrix rules: microfibrillar protein controls vascular development Blood, June 1, 2006; 107(11): 4202 - 4203. [Full Text] [PDF] E. Chen, J. D. Larson, and S. C. Ekker Functional analysis of zebrafish microfibril-associated glycoprotein-1 (Magp1) in vivo reveals roles for microfibrils in vascular development and function Blood, June 1, 2006; 107(11): 4364 - 4374. [Abstract] [Full Text] [PDF] C. Y. Fears, C. L. Gladson, and A. Woods Syndecan-2 Is Expressed in the Microvasculature of Gliomas and Regulates Angiogenic Processes in Microvascular Endothelial Cells J. Biol. Chem., May 26, 2006; 281(21): 14533 - 14536. [Abstract] [Full Text] [PDF] J. M. Miano Fishing for Function in Zebrafish Circ. Res., March 31, 2006; 98(6): 723 - 726. [Full Text] [PDF] C. J.M. Best, J. W. Gillespie, Y. Yi, G. V.R. Chandramouli, M. A. Perlmutter, Y. Gathright, H. S. Erickson, L. Georgevich, M. A. Tangrea, P. H. Duray, et al. Molecular Alterations in Primary Prostate Cancer after Androgen Ablation Therapy Clin. Cancer Res., October 1, 2005; 11(19): 6823 - 6834. [Abstract] [Full Text] [PDF] Y. Kubota, Y. Oike, S. Satoh, Y. Tabata, Y. Niikura, T. Morisada, M. Akao, T. Urano, Y. Ito, T. Miyamoto, et al. Cooperative interaction of Angiopoietin-like proteins 1 and 2 in zebrafish vascular development PNAS, September 20, 2005; 102(38): 13502 - 13507. [Abstract] [Full Text] [PDF] S. Ashikari-Hada, H. Habuchi, Y. Kariya, and K. Kimata Heparin Regulates Vascular Endothelial Growth Factor165-dependent Mitogenic Activity, Tube Formation, and Its Receptor Phosphorylation of Human Endothelial Cells: COMPARISON OF THE EFFECTS OF HEPARIN AND MODIFIED HEPARINS J. Biol. Chem., September 9, 2005; 280(36): 31508 - 31515. [Abstract] [Full Text] [PDF] E. Tkachenko, J. M. Rhodes, and M. Simons Syndecans: New Kids on the Signaling Block Circ. Res., March 18, 2005; 96(5): 488 - 500. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020