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Blood, 15 March 2004, Vol. 103, No. 6, pp. 2062-2070. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-07-2388. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-07-2388v1 103/6/2062    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bond, H. M. Articles by Venuta, S. Search for Related Content PubMed PubMed Citation Articles by Bond, H. M. Articles by Venuta, S. Related Collections Hematopoiesis and Stem Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells Heather M. Bond, Maria Mesuraca, Ennio Carbone, Patrizia Bonelli, Valter Agosti, Nicola Amodio, Gennaro De Rosa, Massimo Di Nicola, Alessandro M. Gianni, Malcolm A. S. Moore, Akiko Hata, Michele Grieco, Giovanni Morrone, and Salvatore Venuta From the Department of Experimental and Clinical Medicine "Gaetano Salvatore," University of Catanzaro Magna, Graecia, Italy; Department of Experimental Oncology, National Cancer Institute Fondazione G. Pascale; Clinical Hematology Divison, University of Napoli Federico II, Napoli, Italy; Cristina Gandini Transplantation Unit, Istituto Nazionale Tumori, Milano, Italy; Molecular Cardiology Research Institute, New England Medical Center, Tufts University School of Medicine, Boston, MA; James Ewing Laboratory of Developmental Hematopoiesis and Department of Developmental Biology, Memorial Sloan-Kettering Cancer Center, New York, NY. Comparison of the gene expression repertoire in human hematopoietic progenitors and mature leukocytes led to identification of a transcript expressed in CD34+cells and undetectable in differentiated cells. Sequencing of the cDNA (termed EHZF: early hematopoietic zinc finger) revealed 30 zinc fingers with 96% homology to mouse Evi3, a recently identified gene associated with the retroviral integration site in AKXD-27 B-cell lymphomas. EHZF and Evi3 share high homology with the transcription cofactor OAZ, implicated in the control of olfactory epithelium and B-lymphocyte differentiation and in the bone morphogenic protein (BMP) signal transduction. Here we show that (1) EHZF expression is abundant in human CD34+ progenitors and declines rapidly during cytokine-driven differentiation; (2) significant mRNA levels are found in most acute myelogenous leukemias; (3) in response to BMPs EHZF complexes SMADs 1 and 4, binds to, and enhances the transcriptional activity of, a BMP2/4 responsive element; (4) EHZF inhibits the transcriptional activity of early B-cell factor (EBF), a transcription factor essential for specification of the B-cell lineage. Taken together, our data suggest that EHZF is likely to play a relevant role in the control of human hematopoiesis and might be implicated in the development of hematopoietic malignancies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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