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Blood, 15 March 2004, Vol. 103, No. 6, pp. 2337-2342. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-09-3277. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-09-3277v1 103/6/2337    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ghia, P. Articles by Caligaris-Cappio, F. Search for Related Content PubMed PubMed Citation Articles by Ghia, P. Articles by Caligaris-Cappio, F. Related Collections Immunobiology Neoplasia Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Monoclonal CD5+ and CD5- B-lymphocyte expansions are frequent in the peripheral blood of the elderly Paolo Ghia, Giuseppina Prato, Cristina Scielzo, Stefania Stella, Massimo Geuna, Giuseppe Guida, and Federico Caligaris-Cappio From the Istituto per la Ricerca e la Cura del Cancro, Candiolo, Italy; Department of Oncological Sciences, University of Torino, Italy; the Division of Clinical Immunology and Hematology, Ospedale Mauriziano "Umberto I," Torino, Italy; and Università Vita-Salute, San Raffaele, Milano, Italy. The responsiveness and diversity of peripheral B-cell repertoire decreases with age, possibly because of B-cell clonal expansions, as suggested by the incidence of serum monoclonal immunoglobulins and of monoclonal chronic lymphocytic leukemia (CLL)–like B lymphocytes in clinically silent adults. We phenotyped peripheral blood cells from 500 healthy subjects older than 65 years with no history or suspicion of malignancies and no evidence of lymphocytosis. In 19 cases (3.8%) a / ratio of more than 3:1 or less than 1:3 was found: 9 were CD5+, CD19+, CD23+, CD20low, CD79blow, sIglow (classic CLL-like phenotype); 3 were CD5+, CD19+, CD23+, CD20high, CD79blow, sIglow (atypical CLL-like), and 7 were CD5-, CD19+, CD20high, CD23-, CD79bbright, FMC7+, sIgbright (non–CLL-like). In 2 subjects, 2 phenotypically distinct unrelated clones were concomitantly evident. No cases were CD10+. Polymerase chain reaction (PCR) analysis demonstrated a monoclonal rearrangement of IgH genes in 15 of 19 cases. No bcl-1 or bcl-2 rearrangements were detected. Using a gating strategy based on CD20/CD5/CD79 expression, 13 additional CLL-like B-cell clones were identified (cumulative frequency of classic CLL-like: 5.5%). Thus, phenotypically heterogeneous monoclonal B-lymphocyte expansions are common among healthy elderly individuals and are not limited to classic CLL-like clones but may have the phenotypic features of different chronic lymphoproliferative disorders, involving also CD5- B cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. D. Shanafelt, N. E. Kay, K. G. Rabe, T. G. Call, C. S. Zent, K. Maddocks, G. Jenkins, D. F. Jelinek, W. G. Morice, J. Boysen, et al. Brief Report: Natural History of Individuals With Clinically Recognized Monoclonal B-Cell Lymphocytosis Compared With Patients With Rai 0 Chronic Lymphocytic Leukemia J. Clin. Oncol., August 20, 2009; 27(24): 3959 - 3963. 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