|
|
Blood, 1 April 2004, Vol. 103, No. 7, pp. 2522-2529.
Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-07-2439.
 Previous Article | Table of Contents | Next Article 
HEMATOPOIESIS
Role of RUNX1 in adult hematopoiesis: analysis of RUNX1-IRES-GFP knock-in mice reveals differential lineage expression
Robert B. Lorsbach,
Jennifer Moore,
Sonny O. Ang,
Weili Sun,
Noel Lenny, and
James R. Downing
From the Department of Pathology, St Jude Children's Research Hospital, Memphis, TN.
The Runx1/core binding factor- (CBF) transcriptional complex is required for the establishment of hematopoiesis during development. Despite its critical role during development, a detailed analysis of Runx1 expression within specific lineages and developmental stages of the adult hematopoietic system is lacking. To address this, we have developed a Runx1—green fluorescent protein (GFP) knock-in mouse. We show that Runx1 is expressed in several hematopoietic lineages, including myeloid, B-lymphoid, and T-lymphoid cells. By contrast, Runx1 is weakly expressed in early erythroid cells, and its expression is rapidly extinguished during later stages of erythropoiesis. Runx1 expression is induced during early B-cell development and is expressed at a uniform level during all subsequent stages of B-cell development. Within the thymus, Runx1 is expressed at the highest level in CD4-CD8- double-negative thymocytes. In peripheral T cells, Runx1 is differentially expressed, with CD4+ T cells expressing 2- to 3-fold higher levels of Runx1 than CD8+ cells. Taken together, these findings indicate that although widely expressed in the hematopoietic system, the expression of Runx1 is regulated in a cell type— and maturation stage—specific manner. In addition, the Runx1-IRES-GFP knock-in mouse strain should prove valuable for investigation of Runx1 function in adult hematopoiesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Wang and R. Bosselut
CD4-CD8 Lineage Differentiation: Thpok-ing into the Nucleus
J. Immunol.,
September 1, 2009;
183(5):
2903 - 2910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Ben-Ami, N. Pencovich, J. Lotem, D. Levanon, and Y. Groner
A regulatory interplay between miR-27a and Runx1 during megakaryopoiesis
PNAS,
January 6, 2009;
106(1):
238 - 243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Speck, M. Chen, T. Yokomizo, B. Zeigler, and E. Dzierzak
Runx1 Interactions in Stem Cell Biology.
Blood (ASH Annual Meeting Abstracts),
November 16, 2008;
112(11):
sci-15 - sci-15.
[Abstract]
|
|
|
|
|
|
|
Y.-H. Kuo, R. M. Gerstein, and L. H. Castilla
Cbf{beta}-SMMHC impairs differentiation of common lymphoid progenitors and reveals an essential role for RUNX in early B-cell development
Blood,
February 1, 2008;
111(3):
1543 - 1551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. F. Wildt, G. Sun, B. Grueter, M. Fischer, M. Zamisch, M. Ehlers, and R. Bosselut
The Transcription Factor Zbtb7b Promotes CD4 Expression by Antagonizing Runx-Mediated Activation of the CD4 Silencer
J. Immunol.,
October 1, 2007;
179(7):
4405 - 4414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Carter, J. M. Lefebvre, D. L. Wiest, and W. G. Tourtellotte
Redundant Role for Early Growth Response Transcriptional Regulators in Thymocyte Differentiation and Survival
J. Immunol.,
June 1, 2007;
178(11):
6796 - 6805.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Miething, R. Grundler, C. Mugler, S. Brero, J. Hoepfl, J. Geigl, M. R. Speicher, O. Ottmann, C. Peschel, and J. Duyster
Retroviral insertional mutagenesis identifies RUNX genes involved in chronic myeloid leukemia disease persistence under imatinib treatment
PNAS,
March 13, 2007;
104(11):
4594 - 4599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cammenga, B. Niebuhr, S. Horn, U. Bergholz, G. Putz, F. Buchholz, J. Lohler, and C. Stocking
RUNX1 DNA-Binding Mutants, Associated with Minimally Differentiated Acute Myelogenous Leukemia, Disrupt Myeloid Differentiation
Cancer Res.,
January 15, 2007;
67(2):
537 - 545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Talebian, Z. Li, Y. Guo, J. Gaudet, M. E. Speck, D. Sugiyama, P. Kaur, W. S. Pear, I. Maillard, and N. A. Speck
T-lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBF{beta} dosage.
Blood,
January 1, 2007;
109(1):
11 - 21.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Vegiopoulos, P. Garcia, N. Emambokus, and J. Frampton
Coordination of erythropoiesis by the transcription factor c-Myb
Blood,
June 15, 2006;
107(12):
4703 - 4710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Hamelin, C. Letourneux, P.-H. Romeo, F. Porteu, and M. Gaudry
Thrombopoietin regulates IEX-1 gene expression through ERK-induced AML1 phosphorylation
Blood,
April 15, 2006;
107(8):
3106 - 3113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Choi, K. E. Elagib, L. L. Delehanty, and A. N. Goldfarb
Erythroid Inhibition by the Leukemic Fusion AML1-ETO Is Associated with Impaired Acetylation of the Major Erythroid Transcription Factor GATA-1.
Cancer Res.,
March 15, 2006;
66(6):
2990 - 2996.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Carella, M. Potter, J. Bonten, J. E. Rehg, G. Neale, and G. C. Grosveld
The ETS factor TEL2 is a hematopoietic oncoprotein
Blood,
February 1, 2006;
107(3):
1124 - 1132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kawagoe and G. C. Grosveld
Conditional MN1-TEL knock-in mice develop acute myeloid leukemia in conjunction with overexpression of HOXA9
Blood,
December 15, 2005;
106(13):
4269 - 4277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kawagoe and G. C. Grosveld
MN1-TEL myeloid oncoprotein expressed in multipotent progenitors perturbs both myeloid and lymphoid growth and causes T-lymphoid tumors in mice
Blood,
December 15, 2005;
106(13):
4278 - 4286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. F. Peterson, A. Boyapati, V. Ranganathan, A. Iwama, D. G. Tenen, S. Tsai, and D.-E. Zhang
The Hematopoietic Transcription Factor AML1 (RUNX1) Is Negatively Regulated by the Cell Cycle Protein Cyclin D3
Mol. Cell. Biol.,
December 1, 2005;
25(23):
10205 - 10219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hirai, I. M Samokhvalov, T. Fujimoto, S. Nishikawa, J. Imanishi, and S.-I. Nishikawa
Involvement of Runx1 in the down-regulation of fetal liver kinase-1 expression during transition of endothelial cells to hematopoietic cells
Blood,
September 15, 2005;
106(6):
1948 - 1955.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Growney, H. Shigematsu, Z. Li, B. H. Lee, J. Adelsperger, R. Rowan, D. P. Curley, J. L. Kutok, K. Akashi, I. R. Williams, et al.
Loss of Runx1 perturbs adult hematopoiesis and is associated with a myeloproliferative phenotype
Blood,
July 15, 2005;
106(2):
494 - 504.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Sun and J. R. Downing
Haploinsufficiency of AML1 results in a decrease in the number of LTR-HSCs while simultaneously inducing an increase in more mature progenitors
Blood,
December 1, 2004;
104(12):
3565 - 3572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
