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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2655-2660. Prepublished online as a Blood First Edition Paper on November 6, 2003; DOI 10.1182/blood-2003-09-3146. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-09-3146v1 103/7/2655    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pennock, J. L. Articles by Grencis, R. K. Search for Related Content PubMed PubMed Citation Articles by Pennock, J. L. Articles by Grencis, R. K. Related Collections Hematopoiesis and Stem Cells Immunobiology Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY In vivo exit of c-kit+/CD49dhi/7+ mucosal mast cell precursors from the bone marrow following infection with the intestinal nematode Trichinella spiralis Joanne L. Pennock, and Richard K. Grencis From the School of Biological Sciences, University of Manchester, Manchester, United Kingdom. We have used the parasite helminth Trichinella spiralis to study the generation and differentiation of mast cell progenitors in the bone marrow of mice, as this infection triggers an intestinal mastocytosis which correlates with parasite expulsion. C-kit+ mast cell progenitors have previously been defined by methylcellulose colony-forming units and by limiting dilution assays in vitro. In vivo experiments have demonstrated the essential requirement by mast cells for specific integrin expression. We have defined 2 c-kit+ populations in the bone marrow, one of which coexpresses CD49d/7 integrin, a marker essential for small intestine immigration. We have confirmed the phenotype of these cells by using antagonistic anti-c-kit antibody in vivo. Our data show that the loss of c-kit+/7+ cells from the bone marrow correlates with their appearance in the blood and precedes detection of mature mast cells in the gut by 3 days. This exit correlates with an increase in soluble stem cell factor (SCF) in the serum, suggesting that the c-kit/SCF interaction may be chemotactic or haptotactic in nature. This study shows that during infection the bone marrow environment generates mast cells destined for the intestinal mucosa before their exit into the periphery, indicating a clear interplay between infection site and hematopoietic tissue. 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