|
|
Blood, 15 April 2004, Vol. 103, No. 8, pp. 2900-2907.
Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-06-1891.
 Previous Article | Table of Contents | Next Article 
CHEMOKINES
Unique SDF-1–induced activation of human precursor-B ALL cells as a result of altered CXCR4 expression and signaling
Asaf Spiegel,
Orit Kollet,
Amnon Peled,
Loya Abel,
Arnon Nagler,
Bella Bielorai,
Gideon Rechavi,
Josef Vormoor, and
Tsvee Lapidot
From the Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel; Gene Therapy Institute, Hadassah University Hospital, Jerusalem, Israel; Departments of Bone Marrow Transplantation and Pediatric Hemato-Oncology, Sheba Medical Center, Ramat-Gan, Israel; Sackler School of Medicine, Tel Aviv University, Tel-Aviv, Israel; and Department of Pediatric Hematology and Oncology, University Children's Hospital, Muenster, Germany.
The mechanisms governing migration and extramedullary dissemination of leukemic cells remain obscure. In this study the migration and in vivo homing to the bone marrow of nonobese diabetic severe combined immunodeficient (NOD/SCID) mice injected with human precursor-B acute lymphoblastic leukemia (ALL) cells in comparison to normal CD34+ progenitors (both cord blood and mobilized peripheral blood) was investigated. Although migration and homing of both cell populations was dependent on stromal cell-derived factor 1 (SDF-1)/CXCR4 interactions, major differences in receptor expression as well as the migratory capacity toward various concentrations of SDF-1 were found. Furthermore, unlike normal CD34+ progenitors, in vivo homing of the leukemic cells was superior when recipient NOD/SCID mice were not irradiated prior to transplantation. In addition, we report differences in the adhesion molecules activated following SDF-1 stimulation, documenting a major role for very late antigen 4 (VLA-4), but not VLA-5 and lymphocyte function-associated antigen-1 (LFA-1), in homing of precursor-B ALL cells. Interestingly, Toxin-B and pertussis toxin inhibited the homing of the leukemic cells but not that of normal CD34+ progenitors or normal CD10+/CD19+ precursor-B cells, revealing differences in CXCR4 signaling pathways that are based on changes that acquired by the leukemic cells. Altogether, our data provide new insights into different SDF-1–induced signaling, activation, and consequent motility between normal CD34+ and precursor-B ALL progenitors, which may lead to improved clinical protocols. (Blood. 2004;103: 2900-2907)
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
X. Wang, W. Zhang, T. Ishii, S. Sozer, J. Wang, M. Xu, and R. Hoffman
Correction of the Abnormal Trafficking of Primary Myelofibrosis CD34+ Cells by Treatment with Chromatin-Modifying Agents
Cancer Res.,
October 1, 2009;
69(19):
7612 - 7618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. N. Hartmann, V. Grabovsky, W. Wang, P. Desch, G. Rubenzer, S. Wollner, I. Binsky, A. Vallon-Eberhard, A. Sapoznikov, M. Burger, et al.
Circulating B-Cell Chronic Lymphocytic Leukemia Cells Display Impaired Migration to Lymph Nodes and Bone Marrow
Cancer Res.,
April 1, 2009;
69(7):
3121 - 3130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. N. Hartmann, V. Grabovsky, R. Pasvolsky, Z. Shulman, E. C. Buss, A. Spiegel, A. Nagler, T. Lapidot, M. Thelen, and R. Alon
A crosstalk between intracellular CXCR7 and CXCR4 involved in rapid CXCL12-triggered integrin activation but not in chemokine-triggered motility of human T lymphocytes and CD34+ cells
J. Leukoc. Biol.,
October 1, 2008;
84(4):
1130 - 1140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Spiegel, E. Zcharia, Y. Vagima, T. Itkin, A. Kalinkovich, A. Dar, O. Kollet, N. Netzer, K. Golan, I. Shafat, et al.
Heparanase regulates retention and proliferation of primitive Sca-1+/c-Kit+/Lin- cells via modulation of the bone marrow microenvironment
Blood,
May 15, 2008;
111(10):
4934 - 4943.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Meads, L. A. Hazlehurst, and W. S. Dalton
The Bone Marrow Microenvironment as a Tumor Sanctuary and Contributor to Drug Resistance
Clin. Cancer Res.,
May 1, 2008;
14(9):
2519 - 2526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhang and L. Li
Stem Cell Niche: Microenvironment and Beyond
J. Biol. Chem.,
April 11, 2008;
283(15):
9499 - 9503.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Alsayed, H. Ngo, J. Runnels, X. Leleu, U. K. Singha, C. M. Pitsillides, J. A. Spencer, T. Kimlinger, J. M. Ghobrial, X. Jia, et al.
Mechanisms of regulation of CXCR4/SDF-1 (CXCL12)-dependent migration and homing in multiple myeloma
Blood,
April 1, 2007;
109(7):
2708 - 2717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Moskovits, A. Kalinkovich, J. Bar, T. Lapidot, and M. Oren
p53 Attenuates Cancer Cell Migration and Invasion through Repression of SDF-1/CXCL12 Expression in Stromal Fibroblasts.
Cancer Res.,
November 15, 2006;
66(22):
10671 - 10676.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kalinkovich, S. Tavor, A. Avigdor, J. Kahn, A. Brill, I. Petit, P. Goichberg, M. Tesio, N. Netzer, E. Naparstek, et al.
Functional CXCR4-Expressing Microparticles and SDF-1 Correlate with Circulating Acute Myelogenous Leukemia Cells.
Cancer Res.,
November 15, 2006;
66(22):
11013 - 11020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lapidot
The Essential Roles of SDF-1/CXCR4 Interactions in Migration and Development of Normal and Leukemic Human Stem Cells, in Chimeric NOD/SCID mice
Am. Assoc. Cancer Res. Educ. Book,
April 1, 2006;
2006(1):
3 - 6.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Foudi, P. Jarrier, Y. Zhang, M. Wittner, J.-F. Geay, Y. Lecluse, T. Nagasawa, W. Vainchenker, and F. Louache
Reduced retention of radioprotective hematopoietic cells within the bone marrow microenvironment in CXCR4-/- chimeric mice
Blood,
March 15, 2006;
107(6):
2243 - 2251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Burger and T. J. Kipps
CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment
Blood,
March 1, 2006;
107(5):
1761 - 1767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Goichberg, A. Kalinkovich, N. Borodovsky, M. Tesio, I. Petit, A. Nagler, I. Hardan, and T. Lapidot
cAMP-induced PKC{zeta} activation increases functional CXCR4 expression on human CD34+ hematopoietic progenitors
Blood,
February 1, 2006;
107(3):
870 - 879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Siliceo, D. Garcia-Bernal, S. Carrasco, E. Diaz-Flores, F. C. Leskow, J. Teixido, M. G. Kazanietz, and I. Merida
{beta}2-chimaerin provides a diacylglycerol-dependent mechanism for regulation of adhesion and chemotaxis of T cells
J. Cell Sci.,
January 1, 2006;
119(1):
141 - 152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lapidot, A. Dar, and O. Kollet
How do stem cells find their way home?
Blood,
September 15, 2005;
106(6):
1901 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tavor, I. Petit, S. Porozov, P. Goichberg, A. Avigdor, S. Sagiv, A. Nagler, E. Naparstek, and T. Lapidot
Motility, proliferation, and egress to the circulation of human AML cells are elastase dependent in NOD/SCID chimeric mice
Blood,
September 15, 2005;
106(6):
2120 - 2127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Grignani, E. Perissinotto, G. Cavalloni, F. Carnevale Schianca, and M. Aglietta
Clinical Use of AMD3100 to Mobilize CD34+ Cells in Patients Affected by Non-Hodgkin's Lymphoma or Multiple Myeloma
J. Clin. Oncol.,
June 1, 2005;
23(16):
3871 - 3872.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bonig, G. V. Priestley, L. M. Nilsson, Y. Jiang, and T. Papayannopoulou
PTX-sensitive signals in bone marrow homing of fetal and adult hematopoietic progenitor cells
Blood,
October 15, 2004;
104(8):
2299 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
