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Blood, 15 April 2004, Vol. 103, No. 8, pp. 2956-2964. Prepublished online as a Blood First Edition Paper on December 30, 2003; DOI 10.1182/blood-2003-09-3314. This Article Full Text Full Text (PDF) Supplemental Figures and Tables All Versions of this Article: 2003-09-3314v1 103/8/2956    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bhattacharya, B. Articles by Puri, R. K. Search for Related Content PubMed PubMed Citation Articles by Bhattacharya, B. Articles by Puri, R. K. Related Collections Hematopoiesis and Stem Cells Gene Expression Genomics Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Gene expression in human embryonic stem cell lines: unique molecular signature Bhaskar Bhattacharya, Takumi Miura, Ralph Brandenberger, Josef Mejido, Yongquan Luo, Amy X. Yang, Bharat H. Joshi, Irene Ginis, R. Scott Thies, Michal Amit, Ian Lyons, Brian G. Condie, Joseph Itskovitz-Eldor, Mahendra S. Rao, and Raj K. Puri From the Laboratory of Molecular Tumor Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD; Laboratory of Neuroscience, National Institute on Aging, Baltimore, MD; Geron Corp, Menlo Park, CA; BresaGen Inc, Athens, GA; Department of Genetics, University of Georgia, Athens; and Department of Obstetrics and Gynecology, The Rambam Medical Center and Faculty of Medicine, Haifa, Israel. Human embryonic stem (huES) cells have the ability to differentiate into a variety of cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. However, little is known about the mechanism of differentiation of huES cells and factors regulating cell development. We have used high-quality microarrays containing 16 659 seventy–base pair oligonucleotides to examine gene expression in 6 of the 11 available huES cell lines. Expression was compared against pooled RNA from multiple tissues (universal RNA) and genes enriched in huES cells were identified. All 6 cell lines expressed multiple markers of the undifferentiated state and shared significant homology in gene expression (overall similarity coefficient > 0.85).A common subset of 92 genes was identified that included Nanog, GTCM-1, connexin 43 (GJA1), oct-4, and TDGF1 (cripto). Gene expression was confirmed by a variety of techniques including comparison with databases, reverse transcriptase–polymerase chain reaction, focused cDNA microarrays, and immunocytochemistry. Comparison with published "stemness" genes revealed a limited overlap, suggesting little similarity with other stem cell populations. Several novel ES cell–specific expressed sequence tags were identified and mapped to the human genome. These results represent the first detailed characterization of undifferentiated huES cells and provide a unique set of markers to profile and better understand the biology of huES cells. (Blood. 2004;103: 2956-2964) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Unique genetics of human embryonic stem cells Dan S. Kaufman Blood 2004 103: 2872. [Full Text] [PDF] This article has been cited by other articles: D. W. McMillin, M. Ooi, J. Delmore, J. Negri, P. Hayden, N. Mitsiades, J. Jakubikova, S.-M. Maira, C. Garcia-Echeverria, R. Schlossman, et al. Antimyeloma Activity of the Orally Bioavailable Dual Phosphatidylinositol 3-Kinase/Mammalian Target of Rapamycin Inhibitor NVP-BEZ235 Cancer Res., July 15, 2009; 69(14): 5835 - 5842. [Abstract] [Full Text] [PDF] S. P. Gong, H. Kim, E. J. Lee, S. T. Lee, S. Moon, H.-J. Lee, and J. M. 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