SEARCH:   Advanced

Blood, 15 April 2004, Vol. 103, No. 8, pp. 2956-2964. Prepublished online as a Blood First Edition Paper on December 30, 2003; DOI 10.1182/blood-2003-09-3314. This Article Full Text Full Text (PDF) Supplemental Figures and Tables All Versions of this Article: 2003-09-3314v1 103/8/2956    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bhattacharya, B. Articles by Puri, R. K. Search for Related Content PubMed PubMed Citation Articles by Bhattacharya, B. Articles by Puri, R. K. Related Collections Hematopoiesis and Stem Cells Gene Expression Genomics Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Gene expression in human embryonic stem cell lines: unique molecular signature Bhaskar Bhattacharya, Takumi Miura, Ralph Brandenberger, Josef Mejido, Yongquan Luo, Amy X. Yang, Bharat H. Joshi, Irene Ginis, R. Scott Thies, Michal Amit, Ian Lyons, Brian G. Condie, Joseph Itskovitz-Eldor, Mahendra S. Rao, and Raj K. Puri From the Laboratory of Molecular Tumor Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD; Laboratory of Neuroscience, National Institute on Aging, Baltimore, MD; Geron Corp, Menlo Park, CA; BresaGen Inc, Athens, GA; Department of Genetics, University of Georgia, Athens; and Department of Obstetrics and Gynecology, The Rambam Medical Center and Faculty of Medicine, Haifa, Israel. Human embryonic stem (huES) cells have the ability to differentiate into a variety of cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. However, little is known about the mechanism of differentiation of huES cells and factors regulating cell development. We have used high-quality microarrays containing 16 659 seventy–base pair oligonucleotides to examine gene expression in 6 of the 11 available huES cell lines. Expression was compared against pooled RNA from multiple tissues (universal RNA) and genes enriched in huES cells were identified. All 6 cell lines expressed multiple markers of the undifferentiated state and shared significant homology in gene expression (overall similarity coefficient > 0.85).A common subset of 92 genes was identified that included Nanog, GTCM-1, connexin 43 (GJA1), oct-4, and TDGF1 (cripto). Gene expression was confirmed by a variety of techniques including comparison with databases, reverse transcriptase–polymerase chain reaction, focused cDNA microarrays, and immunocytochemistry. Comparison with published "stemness" genes revealed a limited overlap, suggesting little similarity with other stem cell populations. Several novel ES cell–specific expressed sequence tags were identified and mapped to the human genome. These results represent the first detailed characterization of undifferentiated huES cells and provide a unique set of markers to profile and better understand the biology of huES cells. (Blood. 2004;103: 2956-2964) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Unique genetics of human embryonic stem cells Dan S. Kaufman Blood 2004 103: 2872. [Full Text] [PDF] This article has been cited by other articles: R. Pal, S. Totey, M. K. Mamidi, V. S. Bhat, and S. Totey Propensity of Human Embryonic Stem Cell Lines During Early Stage of Lineage Specification Controls Their Terminal Differentiation into Mature Cell Types Experimental Biology and Medicine, October 1, 2009; 234(10): 1230 - 1243. [Abstract] [Full Text] [PDF] D. W. McMillin, M. Ooi, J. Delmore, J. Negri, P. Hayden, N. Mitsiades, J. Jakubikova, S.-M. Maira, C. Garcia-Echeverria, R. Schlossman, et al. Antimyeloma Activity of the Orally Bioavailable Dual Phosphatidylinositol 3-Kinase/Mammalian Target of Rapamycin Inhibitor NVP-BEZ235 Cancer Res., July 15, 2009; 69(14): 5835 - 5842. [Abstract] [Full Text] [PDF] S. P. Gong, H. Kim, E. J. Lee, S. T. Lee, S. Moon, H.-J. Lee, and J. M. Lim Change in gene expression of mouse embryonic stem cells derived from parthenogenetic activation Hum. Reprod., April 1, 2009; 24(4): 805 - 814. [Abstract] [Full Text] [PDF] G. Cauffman, M. De Rycke, K. Sermon, I. Liebaers, and H. Van de Velde Markers that define stemness in ESC are unable to identify the totipotent cells in human preimplantation embryos Hum. Reprod., January 1, 2009; 24(1): 63 - 70. [Abstract] [Full Text] [PDF] D. Xie, J. Cai, N.-Y. Chia, H. H. Ng, and S. Zhong Cross-species de novo identification of cis-regulatory modules with GibbsModule: Application to gene regulation in embryonic stem cells Genome Res., August 1, 2008; 18(8): 1325 - 1335. [Abstract] [Full Text] [PDF] R. D. Palmer, N. L. Barbosa-Morais, E. L. Gooding, B. Muralidhar, C. M. Thornton, M. R. Pett, I. Roberts, D. T. Schneider, N. Thorne, S. Tavare, et al. Pediatric Malignant Germ Cell Tumors Show Characteristic Transcriptome Profiles Cancer Res., June 1, 2008; 68(11): 4239 - 4247. [Abstract] [Full Text] [PDF] S J Kimber, S F Sneddon, D J Bloor, A M El-Bareg, J A Hawkhead, A D Metcalfe, F D Houghton, H J Leese, A Rutherford, B A Lieberman, et al. Expression of genes involved in early cell fate decisions in human embryos and their regulation by growth factors Reproduction, May 1, 2008; 135(5): 635 - 647. [Abstract] [Full Text] [PDF] R. D. Morin, M. D. O'Connor, M. Griffith, F. Kuchenbauer, A. Delaney, A.-L. Prabhu, Y. Zhao, H. McDonald, T. Zeng, M. Hirst, et al. Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells Genome Res., April 1, 2008; 18(4): 610 - 621. [Abstract] [Full Text] [PDF] W. E. Lowry, L. Richter, R. Yachechko, A. D. Pyle, J. Tchieu, R. Sridharan, A. T. Clark, and K. Plath Generation of human induced pluripotent stem cells from dermal fibroblasts PNAS, February 26, 2008; 105(8): 2883 - 2888. [Abstract] [Full Text] [PDF] S. M. Tan, S. T. Wang, H. Hentze, and P. Droge A UTF1-based selection system for stable homogeneously pluripotent human embryonic stem cell cultures Nucleic Acids Res., September 25, 2007; 35(18): e118 - e118. [Abstract] [Full Text] [PDF] S. Chateauvieux, J.-L. Ichante, B. Delorme, V. Frouin, G. Pietu, A. Langonne, N. Gallay, L. Sensebe, M. T. Martin, K. A. Moore, et al. Molecular profile of mouse stromal mesenchymal stem cells Physiol Genomics, April 24, 2007; 29(2): 128 - 138. [Abstract] [Full Text] [PDF] C. Allegrucci and L.E. Young Differences between human embryonic stem cell lines Hum. Reprod. Update, March 1, 2007; 13(2): 103 - 120. [Abstract] [Full Text] [PDF] J. A. Byrne, S. M. Mitalipov, L. Clepper, and D. P. Wolf Transcriptional Profiling of Rhesus Monkey Embryonic Stem Cells Biol Reprod, December 1, 2006; 75(6): 908 - 915. [Abstract] [Full Text] [PDF] N. Auger, J. Thillet, K. Wanherdrick, A. Idbaih, M.-E. Legrier, B. Dutrillaux, M. Sanson, and M.-F. Poupon Genetic alterations associated with acquired temozolomide resistance in SNB-19, a human glioma cell line. Mol. Cancer Ther., September 1, 2006; 5(9): 2182 - 2192. [Abstract] [Full Text] [PDF] W. Shi, H. Wang, G. Pan, Y. Geng, Y. Guo, and D. Pei Regulation of the Pluripotency Marker Rex-1 by Nanog and Sox2 J. Biol. Chem., August 18, 2006; 281(33): 23319 - 23325. [Abstract] [Full Text] [PDF] L. Armstrong, O. Hughes, S. Yung, L. Hyslop, R. Stewart, I. Wappler, H. Peters, T. Walter, P. Stojkovic, J. Evans, et al. The role of PI3K/AKT, MAPK/ERK and NF{kappa}{beta} signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis Hum. Mol. Genet., June 1, 2006; 15(11): 1894 - 1913. [Abstract] [Full Text] [PDF] A. L. Olsen, D. L. Stachura, and M. J. Weiss Designer blood: creating hematopoietic lineages from embryonic stem cells Blood, February 15, 2006; 107(4): 1265 - 1275. [Abstract] [Full Text] [PDF] C. Chen, S. M. Ware, A. Sato, D. E. Houston-Hawkins, R. Habas, M. M. Matzuk, M. M. Shen, and C. W. Brown The Vg1-related protein Gdf3 acts in a Nodal signaling pathway in the pre-gastrulation mouse embryo Development, January 15, 2006; 133(2): 319 - 329. [Abstract] [Full Text] [PDF] L. G. Puente, D. J. Borris, J.-F. Carriere, J. F. Kelly, and L. A. Megeney Identification of Candidate Regulators of Embryonic Stem Cell Differentiation by Comparative Phosphoprotein Affinity Profiling Mol. Cell. Proteomics, January 1, 2006; 5(1): 57 - 67. [Abstract] [Full Text] [PDF] T. Enver, S. Soneji, C. Joshi, J. Brown, F. Iborra, T. Orntoft, T. Thykjaer, E. Maltby, K. Smith, R. A. Dawud, et al. Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells Hum. Mol. Genet., November 1, 2005; 14(21): 3129 - 3140. [Abstract] [Full Text] [PDF] C. A. Cowan, J. Atienza, D. A. Melton, and K. Eggan Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells Science, August 26, 2005; 309(5739): 1369 - 1373. [Abstract] [Full Text] [PDF] R. I. Skotheim, G. E. Lind, O. Monni, J. M. Nesland, V. M. Abeler, S. D. Fossa, N. Duale, G. Brunborg, O. Kallioniemi, P. W. Andrews, et al. Differentiation of Human Embryonal Carcinomas In vitro and In vivo Reveals Expression Profiles Relevant to Normal Development Cancer Res., July 1, 2005; 65(13): 5588 - 5598. [Abstract] [Full Text] [PDF] A. M. Wobus and K. R. Boheler Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy Physiol Rev, April 1, 2005; 85(2): 635 - 678. [Abstract] [Full Text] [PDF] M. F. Pera and A. O. Trounson Human embryonic stem cells: prospects for development Development, November 15, 2004; 131(22): 5515 - 5525. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020