SEARCH:   Advanced

Blood, 15 April 2004, Vol. 103, No. 8, pp. 3029-3037. Prepublished online as a Blood First Edition Paper on January 8, 2004; DOI 10.1182/blood-2003-10-3417. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-10-3417v1 103/8/3029    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hjortoe, G. M. Articles by Rao, L. V. M. Search for Related Content PubMed PubMed Citation Articles by Hjortoe, G. M. Articles by Rao, L. V. M. Related Collections Hemostasis, Thrombosis, and Vascular Biology Neoplasia Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Tissue factor-factor VIIa–specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration Gertrud M. Hjortoe, Lars C. Petersen, Tatjana Albrektsen, Brit B. Sorensen, Peder L. Norby, Samir K. Mandal, Usha R. Pendurthi, and L. Vijaya Mohan Rao From the Biomedical Research Division, University of Texas Health Center at Tyler, TX; and Health Care Discovery, Novo Nordisk A/S, Maaloev and Bagsvaerd, Denmark. Tissue factor (TF), the cellular receptor for factor VIIa (FVIIa), besides initiating blood coagulation, is believed to play an important role in tissue repair, inflammation, angiogenesis, and tumor metastasis. Like TF, the chemokine interleukin-8 (IL-8) is shown to play a critical role in these processes. To elucidate the potential mechanisms by which TF contributes to tumor invasion and metastasis, we investigated the effect of FVIIa on IL-8 expression and cell migration in a breast carcinoma cell line, MDA-MB-231, a cell line that constitutively expresses abundant TF. Expression of IL-8 mRNA in MDA-MB-231 cells was markedly up-regulated by plasma concentrations of FVII or an equivalent concentration of FVIIa (10 nM). Neither thrombin nor other proteases involved in hemostasis were effective in stimulating IL-8 in these cells. Increased transcriptional activation of the IL-8 gene is responsible for increased expression of IL-8 in FVIIa-treated cells. PAR-2–specific antibodies fully attenuated TF-FVIIa–induced IL-8 expression. Additional in vitro experiments showed that TF-FVIIa promoted tumor cell migration and invasion, active site–inactivated FVIIa, and specific antibodies against TF, PAR-2, and IL-8 inhibited TF-FVIIa–induced cell migration. In summary, the studies described herein provide insight into how TF may contribute to tumor invasion. (Blood. 2004;103:3029-3037) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Siegbahn, M. Johnell, A. Nordin, M. Aberg, and T. Velling TF/FVIIa Transactivate PDGFR to Regulate PDGF-BB Induced Chemotaxis in Different Cell Types: Involvement of Src And PLC Arterioscler. Thromb. Vasc. Biol., January 1, 2008; 28(1): 135 - 141. [Abstract] [Full Text] [PDF] H. H. Versteeg, F. Schaffner, M. Kerver, H. H. Petersen, J. Ahamed, B. Felding-Habermann, Y. Takada, B. M. Mueller, and W. Ruf Inhibition of tissue factor signaling suppresses tumor growth Blood, January 1, 2008; 111(1): 190 - 199. [Abstract] [Full Text] [PDF] U. R. Pendurthi, S. Ghosh, S. K. Mandal, and L. V. M. Rao Tissue factor activation: is disulfide bond switching a regulatory mechanism? Blood, December 1, 2007; 110(12): 3900 - 3908. [Abstract] [Full Text] [PDF] M. Zoudilova, P. Kumar, L. Ge, P. Wang, G. M. Bokoch, and K. A. DeFea beta-Arrestin-dependent Regulation of the Cofilin Pathway Downstream of Protease-activated Receptor-2 J. Biol. Chem., July 13, 2007; 282(28): 20634 - 20646. [Abstract] [Full Text] [PDF] M. Wygrecka, P. Markart, C. Ruppert, K. Petri, K. T. Preissner, W. Seeger, and A. Guenther Cellular origin of pro-coagulant and (anti)-fibrinolytic factors in bleomycin-injured lungs Eur. Respir. J., June 1, 2007; 29(6): 1105 - 1114. [Abstract] [Full Text] [PDF] V. Awasthi, S. K. Mandal, V. Papanna, L. V. M. Rao, and U. R. Pendurthi Modulation of Tissue Factor-Factor VIIa Signaling by Lipid Rafts and Caveolae Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1447 - 1455. [Abstract] [Full Text] [PDF] P. Arora, T. K. Ricks, and J. Trejo Protease-activated receptor signalling, endocytic sorting and dysregulation in cancer J. Cell Sci., March 15, 2007; 120(6): 921 - 928. [Abstract] [Full Text] [PDF] J. Sevastos, S. E. Kennedy, D. R. Davis, M. Sam, P. W. Peake, J. A. Charlesworth, N. Mackman, and J. H. Erlich Tissue factor deficiency and PAR-1 deficiency are protective against renal ischemia reperfusion injury Blood, January 15, 2007; 109(2): 577 - 583. [Abstract] [Full Text] [PDF] S. Koizume, M.-S. Jin, E. Miyagi, F. Hirahara, Y. Nakamura, J.-H. Piao, A. Asai, A. Yoshida, E. Tsuchiya, W. Ruf, et al. Activation of Cancer Cell Migration and Invasion by Ectopic Synthesis of Coagulation Factor VII Cancer Res., October 1, 2006; 66(19): 9453 - 9460. [Abstract] [Full Text] [PDF] J. Ahamed, H. H. Versteeg, M. Kerver, V. M. Chen, B. M. Mueller, P. J. Hogg, and W. Ruf Disulfide isomerization switches tissue factor from coagulation to cell signaling PNAS, September 19, 2006; 103(38): 13932 - 13937. [Abstract] [Full Text] [PDF] H. C. Lane, A. R. Anand, and R. K. Ganju Cbl and Akt regulate CXCL8-induced and CXCR1- and CXCR2-mediated chemotaxis Int. Immunol., August 1, 2006; 18(8): 1315 - 1325. [Abstract] [Full Text] [PDF] M. Amarzguioui, Q. Peng, M. T. Wiiger, V. Vasovic, E. Babaie, T. Holen, J. M. Nesland, and H. Prydz Ex vivo and In vivo Delivery of Anti-Tissue Factor Short Interfering RNA Inhibits Mouse Pulmonary Metastasis of B16 Melanoma Cells. Clin. Cancer Res., July 1, 2006; 12(13): 4055 - 4061. [Abstract] [Full Text] [PDF] T. Zhu, F. Sennlaub, M. H. Beauchamp, L. Fan, J. S. Joyal, D. Checchin, S. Nim, P. Lachapelle, M. Sirinyan, X. Hou, et al. Proangiogenic Effects of Protease-Activated Receptor 2 Are Tumor Necrosis Factor-{alpha} and Consecutively Tie2 Dependent Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 744 - 750. [Abstract] [Full Text] [PDF] T. M. Hackeng, K. M. Sere, G. Tans, and J. Rosing Protein S stimulates inhibition of the tissue factor pathway by tissue factor pathway inhibitor PNAS, February 28, 2006; 103(9): 3106 - 3111. [Abstract] [Full Text] [PDF] D. R. Morris, Y. Ding, T. K. Ricks, A. Gullapalli, B. L. Wolfe, and J. Trejo Protease-Activated Receptor-2 Is Essential for Factor VIIa and Xa-Induced Signaling, Migration, and Invasion of Breast Cancer Cells Cancer Res., January 1, 2006; 66(1): 307 - 314. [Abstract] [Full Text] [PDF] K. E. Welty-Wolf, M. S. Carraway, T. L. Ortel, A. J. Ghio, S. Idell, J. Egan, X. Zhu, J.-a. Jiao, H. C. Wong, and C. A. Piantadosi Blockade of tissue factor-factor X binding attenuates sepsis-induced respiratory and renal failure Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L21 - L31. [Abstract] [Full Text] [PDF] S.-W. Kim, M. Hayashi, J.-F. Lo, C. Fearns, R. Xiang, G. Lazennec, Y. Yang, and J.-D. Lee Tid1 Negatively Regulates the Migratory Potential of Cancer Cells by Inhibiting the Production of Interleukin-8 Cancer Res., October 1, 2005; 65(19): 8784 - 8791. [Abstract] [Full Text] [PDF] U. Johansson, C. Lawson, M. Dabare, D. Syndercombe-Court, A. C. Newland, G. L. Howells, and M. G. Macey Human peripheral blood monocytes express protease receptor-2 and respond to receptor activation by production of IL-6, IL-8, and IL-1{beta} J. Leukoc. Biol., October 1, 2005; 78(4): 967 - 975. [Abstract] [Full Text] [PDF] M. Belting, J. Ahamed, and W. Ruf Signaling of the Tissue Factor Coagulation Pathway in Angiogenesis and Cancer Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1545 - 1550. [Abstract] [Full Text] [PDF] F. Noorbakhsh, N. Vergnolle, J. C. McArthur, C. Silva, M. Vodjgani, P. Andrade-Gordon, M. D. Hollenberg, and C. Power Proteinase-Activated Receptor-2 Induction by Neuroinflammation Prevents Neuronal Death during HIV Infection J. Immunol., June 1, 2005; 174(11): 7320 - 7329. [Abstract] [Full Text] [PDF] H. W. van Deventer, W. O'Connor Jr., W. J. Brickey, R. M. Aris, J. P.Y. Ting, and J. S. Serody C-C Chemokine Receptor 5 on Stromal Cells Promotes Pulmonary Metastasis Cancer Res., April 15, 2005; 65(8): 3374 - 3379. [Abstract] [Full Text] [PDF] J. Ahamed, M. Belting, and W. Ruf Regulation of tissue factor-induced signaling by endogenous and recombinant tissue factor pathway inhibitor 1 Blood, March 15, 2005; 105(6): 2384 - 2391. [Abstract] [Full Text] [PDF] L. V. M. Rao and U. R. Pendurthi Tissue Factor-Factor VIIa Signaling Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 47 - 56. [Abstract] [Full Text] [PDF] L. Ge, S. K. Shenoy, R. J. Lefkowitz, and K. DeFea Constitutive Protease-activated Receptor-2-mediated Migration of MDA MB-231 Breast Cancer Cells Requires Both {beta}-Arrestin-1 and -2 J. Biol. Chem., December 31, 2004; 279(53): 55419 - 55424. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020