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Blood, 15 April 2004, Vol. 103, No. 8, pp. 3058-3064. Prepublished online as a Blood First Edition Paper on December 30, 2003; DOI 10.1182/blood-2003-08-2972. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-08-2972v1 103/8/3058    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Poeck, H. Articles by Hartmann, G. Search for Related Content PubMed PubMed Citation Articles by Poeck, H. Articles by Hartmann, G. Related Collections Immunobiology Phagocytes Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T-cell help Hendrik Poeck, Moritz Wagner, Julia Battiany, Simon Rothenfusser, Daniela Wellisch, Veit Hornung, Bernd Jahrsdorfer, Thomas Giese, Stefan Endres, and Gunther Hartmann From the Department of Internal Medicine, Division of Clinical Pharmacology, Ludwig-Maximilians-University of Munich, Germany; and Institute of Immunology, University of Heidelberg, Germany. It has been reported that interferon (IFN-) enhances humoral immunity and that dendritic cells of the myeloid lineage promote B-cell differentiation. Here we studied whether the plasmacytoid dendritic cell (PDC), a subset of dendritic cells specialized for the production of IFN-, is involved in regulating B-cell differentiation and immunoglobulin production. The recently identified class of CpG oligonucleotides (CpG-C) was used to activate both B cells and PDCs via Toll-like receptor 9 (TLR9). The presence of PDCs synergistically enhanced CD86 expression, cytokine production (interleukin 6 [IL-6], tumor necrosis factor , and IL-10) and plasma cell differentiation of isolated human peripheral blood B cells stimulated through CpG-C and B-cell antigen receptor (BCR) ligation. This stimulation protocol was sufficient to drive purified naive B cells into IgM-producing plasma cells and to trigger IgG synthesis in memory B cells. PDCs contributed to B-cell activation via IFN- secretion. Up-regulation of TLR9 on B cells was not involved. These results demonstrate that CpG-stimulated PDCs induce plasma cell differentiation in naive and memory B cells in the absence of T-cell help, providing an explanation for the excellent activity of CpG oligonucleotides as a humoral vaccine adjuvant. (Blood. 2004;103:3058-3064) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Ding, Y. Cai, J. Marroquin, S. T. Ildstad, and J. Yan Plasmacytoid Dendritic Cells Regulate Autoreactive B Cell Activation via Soluble Factors and in a Cell-to-Cell Contact Manner J. Immunol., December 1, 2009; 183(11): 7140 - 7149. [Abstract] [Full Text] [PDF] Y. Gao, B. Majchrzak-Kita, E. N. Fish, and J. L. Gommerman Dynamic accumulation of plasmacytoid dendritic cells in lymph nodes is regulated by interferon-{beta} Blood, September 24, 2009; 114(13): 2623 - 2631. [Abstract] [Full Text] [PDF] L. Giordani, M. Sanchez, I. Libri, M. G. Quaranta, B. Mattioli, and M. 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