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Blood, 15 April 2004, Vol. 103, No. 8, pp. 3111-3116. Prepublished online as a Blood First Edition Paper on December 24, 2003; DOI 10.1182/blood-2003-08-2717. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-08-2717v1 103/8/3111    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marelli-Berg, F. M. Articles by Lechler, R. I. Search for Related Content PubMed PubMed Citation Articles by Marelli-Berg, F. M. Articles by Lechler, R. I. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Cognate recognition of the endothelium induces HY-specific CD8+ T-lymphocyte transendothelial migration (diapedesis) in vivo Federica M. Marelli-Berg, Martha J. James, John Dangerfield, Julian Dyson, Maggie Millrain, Diane Scott, Elizabeth Simpson, Sussan Nourshargh, and Robert I. Lechler From the Department of Immunology, Division of Medicine, Imperial College London; the Cardiovascular Medicine Unit, Hammersmith Hospital Campus, Imperial College London, London, United Kingdom; and Transplantation Biology, MRC Clinical Sciences Centre, London, United Kingdom. The physiologic significance of MHC-peptide complex presentation by endothelial cells (ECs) to trafficking T lymphocytes remains unresolved. On the basis of our observation that cognate recognition of ECs enhanced transendothelial migration of antigen-specific T lymphocytes in vitro, we have proposed that by displaying antigenic peptides from the underlying tissue, ECs promote the recruitment of antigen-specific T cells. In this study, we have tested this hypothesis by comparing the trafficking of HY-specific T lymphocytes into antigenic and nonantigenic tissue using in vivo models of T-cell recruitment. Up-regulated expression of H2 molecules presenting endogenous antigen in the peritoneal mesothelium and vessels led to the local recruitment of HY-specific T cells in male, but not female, mice. Intravital microscopy experiments analyzing EC–HY-specific T-cell interactions in the cremasteric vascular bed revealed that cognate recognition of the endothelium results in enhanced diapedesis of T cells into the tissue, while not affecting rolling and adhesion. Our results are consistent with the hypothesis that, under inflammatory conditions, antigen presentation by the endothelium contributes to the development and specificity of T-cell–mediated inflammation by favoring the selective migration of antigen-specific T cells. (Blood. 2004;103:3111-3116) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. David, L. Ma, A. Ivetic, A. Takesono, A. J. Ridley, J.-G. Chai, V. L. Tybulewicz, and F. M. Marelli-Berg T-cell receptor- and CD28-induced Vav1 activity is required for the accumulation of primed T cells into antigenic tissue Blood, April 16, 2009; 113(16): 3696 - 3705. [Abstract] [Full Text] [PDF] B. Lozanoska-Ochser, N. J. Klein, G. C. Huang, R. A. Alvarez, and M. Peakman Expression of CD86 on Human Islet Endothelial Cells Facilitates T Cell Adhesion and Migration J. Immunol., November 1, 2008; 181(9): 6109 - 6116. [Abstract] [Full Text] [PDF] T. D. Manes and J. S. Pober Antigen Presentation by Human Microvascular Endothelial Cells Triggers ICAM-1-Dependent Transendothelial Protrusion by, and Fractalkine-Dependent Transendothelial Migration of, Effector Memory CD4+ T Cells J. Immunol., June 15, 2008; 180(12): 8386 - 8392. [Abstract] [Full Text] [PDF] I. Galea, M. Bernardes-Silva, P. A. Forse, N. van Rooijen, R. S. Liblau, and V. H. Perry An antigen-specific pathway for CD8 T cells across the blood-brain barrier J. Exp. Med., September 3, 2007; 204(9): 2023 - 2030. [Abstract] [Full Text] [PDF] V. Mirenda, S. J. Jarmin, R. David, J. Dyson, D. Scott, Y. Gu, R. I. Lechler, K. Okkenhaug, and F. M. Marelli-Berg Physiologic and aberrant regulation of memory T-cell trafficking by the costimulatory molecule CD28 Blood, April 1, 2007; 109(7): 2968 - 2977. [Abstract] [Full Text] [PDF] T. D. Manes, S. L. Shiao, T. J. Dengler, and J. S. Pober TCR Signaling Antagonizes Rapid IP-10-Mediated Transendothelial Migration of Effector Memory CD4+ T Cells J. Immunol., March 1, 2007; 178(5): 3237 - 3243. [Abstract] [Full Text] [PDF] D. A. Carlow and H. J. Ziltener CD43 Deficiency Has No Impact in Competitive In Vivo Assays of Neutrophil or Activated T Cell Recruitment Efficiency J. Immunol., November 1, 2006; 177(9): 6450 - 6459. [Abstract] [Full Text] [PDF] M. Kummer, A. Lev, Y. Reiter, and B. C. Biedermann Vascular Endothelial Cells Have Impaired Capacity to Present Immunodominant, Antigenic Peptides: A Mechanism of Cell Type-Specific Immune Escape J. Immunol., February 15, 2005; 174(4): 1947 - 1953. [Abstract] [Full Text] [PDF]

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