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Blood, 15 April 2004, Vol. 103, No. 8, pp. 3208-3215. Prepublished online as a Blood First Edition Paper on January 8, 2004; DOI 10.1182/blood-2003-07-2335. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-07-2335v1 103/8/3208    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zompi, S. Articles by Delfau-Larue, M.-H. Search for Related Content PubMed PubMed Citation Articles by Zompi, S. Articles by Delfau-Larue, M.-H. Related Collections Clinical Trials and Observations Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Clonality analysis of alveolar B lymphocytes contributes to the diagnostic strategy in clinical suspicion of pulmonary lymphoma Simona Zompi, Louis-Jean Couderc, Jacques Cadranel, Martine Antoine, Bernard Epardeau, Jocelyne Fleury-Feith, Natalia Popa, François Santoli, Jean-Pierre Farcet, and Marie-Hélène Delfau-Larue From the Services de Pneumologie and d'Anatomopathologie, Hôpital Foch, Suresnes, France; Services de Pneumologie, d'Anatomopathologie, and d'Histologie-Biologie Tumorale, Hôpital Tenon, AP-HP, EA3493, Université Paris VI, Paris, France; and Service d'Immunologie Biologique, Hôpital Henri Mondor, VI AP-HP, EA2348, Université Paris XII, Créteil, France. The diagnostic procedure of chronic pulmonary opacities may envisage the search for non-Hodgkin lymphoma (NHL). Previous retrospective studies have shown that clonality analysis of bronchoalveolar B lymphocytes could reflect the clonality of pulmonary lymphocytes. Our objective was to define the diagnostic usefulness of bronchoalveolar lavage (BAL) B-lymphocyte clonality analysis in the setting of a clinical suspicion of both primary and secondary pulmonary lymphoma. A prospective BAL fluid B-cell clonality analysis was performed by polymerase chain reaction (PCR) in 106 consecutive patients presenting with a clinical suspicion of pulmonary NHL. Diagnosis was pulmonary B-cell lymphoma for 22 patients (13 primary and 9 secondary). When compared, pulmonary biopsy and BAL fluid have clonal identity. The detection of a strong B-cell clonal population in BAL fluid was associated with the diagnosis of pulmonary NHL (P < .0001), with a 97% specificity and a 95% negative predictive value. Thus, the absence of a dominant B-cell clone detection in BAL fluid could help to dismiss invasive investigations of pulmonary lesions. The detection of a dominant B-cell clone would lead to the performance of a pulmonary biopsy to get histologic diagnosis in primary pulmonary lymphoma and, by contrast, would avoid the need for biopsy in the setting of a secondary pulmonary lymphoma. (Blood. 2004;103: 3208-3215) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Colombat, H. Mal, C. Copie-Bergman, J. Diebold, D. Damotte, P. Callard, M. Fournier, J.-P. Farcet, M. Stern, and M.-H. Delfau-Larue Primary cystic lung light chain deposition disease: a clinicopathologic entity derived from unmutated B cells with a stereotyped IGHV4-34/IGKV1 receptor Blood, September 1, 2008; 112(5): 2004 - 2012. [Abstract] [Full Text] [PDF] I. Ito, S. Nagai, M. Kitaichi, A. G. Nicholson, T. Johkoh, S. Noma, D. S. Kim, T. Handa, T. Izumi, and M. Mishima Pulmonary Manifestations of Primary Sjogren's Syndrome: A Clinical, Radiologic, and Pathologic Study Am. J. Respir. Crit. Care Med., March 15, 2005; 171(6): 632 - 638. [Abstract] [Full Text] [PDF]

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