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Blood, 1 May 2004, Vol. 103, No. 9, pp. 3287-3295. Prepublished online as a Blood First Edition Paper on January 15, 2004; DOI 10.1182/blood-2003-07-2428. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-07-2428v1 103/9/3287    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carlo-Stella, C. Articles by Gianni, A. M. Search for Related Content PubMed PubMed Citation Articles by Carlo-Stella, C. Articles by Gianni, A. M. Related Collections Hematopoiesis and Stem Cells Transplantation Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CLINICAL OBSERVATIONS, INTERVENTIONS, AND THERAPEUTIC TRIALS Use of recombinant human growth hormone (rhGH) plus recombinant human granulocyte colony-stimulating factor (rhG-CSF) for the mobilization and collection of CD34+ cells in poor mobilizers Carmelo Carlo-Stella, Massimo Di Nicola, Raffaella Milani, Anna Guidetti, Michele Magni, Marco Milanesi, Paolo Longoni, Paola Matteucci, Franca Formelli, Fernando Ravagnani, Paolo Corradini, and Alessandro M. Gianni From the Cristina Gandini Medical Oncology Unit, Hematology and Bone Marrow Transplantation Unit, the Department of Experimental Oncology, Division of Immunohematology and Transfusion Medicine, Istituto Nazionale Tumori, Milano, Italy; and the Departments of Hematology and Medical Oncology, University of Milano, Milano, Italy. The activity of recombinant human growth hormone (rhGH) in enhancing CD34+ cell mobilization elicited by chemotherapy plus recombinant human granulocyte colony-stimulating factor (rhG-CSF) was evaluated in 16 hard-to-mobilize patients, that is, those achieving a peak of circulating CD34+ cells 10/µL or less, or a collection of CD34+ cells equal to or less than 2 x 106/kg. Patients who had failed a first mobilization attempt with chemotherapy plus rhG-CSF (5 µg/kg/d) were remobilized with chemotherapy plus rhG-CSF and rhGH (100 µg/kg/d). As compared with rhG-CSF, the combined rhGH/rhG-CSF treatment induced significantly higher (P .05) median peak values for CD34+ cells/µL (7 versus 29), colony-forming cells (CFCs)/mL (2154 versus 28 510), and long-term culture-initiating cells (LTC-ICs)/mL (25 versus 511). Following rhG-CSF and rhGH/rhG-CSF, the median yields of CD34+ cells per leukapheresis were 1.1 x 106/kg and 2.3 x 106/kg (P .008), respectively; the median total collections of CD34+ cells were 1.1 x 106/kg and 6 x 106/kg (P .008), respectively. No specific side effect could be ascribed to rhGH, except a transient hyperglycemia occurring in 2 patients. Reinfusion of rhGH/rhG-CSF-mobilized cells following myeloablative therapy resulted in prompt hematopoietic recovery. In conclusion, our data demonstrate that in poor mobilizers addition of rhGH to rhG-CSF allows the patients to efficiently mobilize and collect CD34+ cells with maintained functional properties. (Blood. 2004;103: 3287-3295) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A van der Klaauw, A. M Pereira, T. J Rabelink, E. P M Corssmit, A.-J. Zonneveld, H. Pijl, H. C de Boer, J. W A Smit, J. A Romijn, and E. J P de Koning Recombinant human GH replacement increases CD34+ cells and improves endothelial function in adults with GH deficiency Eur. J. Endocrinol., August 1, 2008; 159(2): 105 - 111. [Abstract] [Full Text] [PDF] D. Focosi, R. E. Kast, S. Galimberti, and M. Petrini Conditioning response to granulocyte colony-stimulating factor via the dipeptidyl peptidase IV-adenosine deaminase complex J. Leukoc. Biol., August 1, 2008; 84(2): 331 - 337. [Abstract] [Full Text] [PDF] T. Papayannopoulou and D. T. Scadden Stem-cell ecology and stem cells in motion Blood, April 15, 2008; 111(8): 3923 - 3930. [Abstract] [Full Text] [PDF] R. M. Lemoli and A. D'Addio Hematopoietic stem cell mobilization Haematologica, March 1, 2008; 93(3): 321 - 324. [Full Text] [PDF] O. M. Pello, M. del Carmen Moreno-Ortiz, J. M. Rodriguez-Frade, L. Martinez-Munoz, D. Lucas, L. Gomez, P. Lucas, E. Samper, M. Aracil, C. Martinez-A, et al. SOCS up-regulation mobilizes autologous stem cells through CXCR4 blockade Blood, December 1, 2006; 108(12): 3928 - 3937. [Abstract] [Full Text] [PDF]

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