SEARCH:   Advanced

Blood, 1 May 2004, Vol. 103, No. 9, pp. 3396-3402. Prepublished online as a Blood First Edition Paper on January 22, 2004; DOI 10.1182/blood-2003-10-3721. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-10-3721v1 103/9/3396    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Li, T.-T. Articles by Bray, P. F. Search for Related Content PubMed PubMed Citation Articles by Li, T.-T. Articles by Bray, P. F. Related Collections Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Signal Transduction Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Genetic variation responsible for mouse strain differences in integrin 2 expression is associated with altered platelet responses to collagen Tong-Tong Li, Susana Larrucea, Shiloe Souza, Suzanne M. Leal, José A. López, Edward M. Rubin, Bernhard Nieswandt, and Paul F. Bray From the Departments of Medicine and Molecular and Human Genetics, Baylor College of Medicine, Houston, TX; the Lawrence Berkeley Laboratories, Berkeley, CA; and the Department of Vascular Biology, Rudolf Virchow Center for Experimental Biomedicine Versbacher, Würzburg, Germany. As mouse models have become commonplace for studying hemostasis and thrombosis, we considered whether the mouse system had utility for assessing genetic alterations in platelet receptors. Platelets from 5 mouse strains (C57BL/6 [C57], FVB/N [FVB], BALB/c, C3H/He, and 129Sv) showed only minor differences in the expression of integrin IIb, integrin 3, glycoprotein (GP) Ib, or GPVI across strains. However, FVB platelets expressed approximately 50% the level of integrin 2 as platelets from other strains (P < .0001). We bred FVB mice with C57 and assessed 2 expression in FVB/C57xFVB/C57 (F2) offspring. Linkage analysis demonstrated the gene responsible for 2 levels is tightly linked to the D13mit260 marker (log odds [lod] score 6.7) near the 2 gene. FVB platelets showed reduced aggregation and a longer lag phase to collagen. FVB and C57 platelets aggregated similarly to collagen-related peptide, but FVB platelets showed a reduction in rhodocytin-induced Syk and PLC2 tyrosine phosphorylation. Thus, FVB platelets express half the level of 2 as other mouse strains, a trait linked to the 2 gene and seemingly responsible for reduced platelet aggregation to collagen. These strain differences serve as a useful model for the 2-fold difference in human platelet 21 expression and demonstrate that 21 participates in signaling during platelet activation. (Blood. 2004;103:3396-3402) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Of men; why not mice? Thomas J. Kunicki Blood 2004 103: 3251-3252. [Full Text] [PDF] This article has been cited by other articles: M. Mazzucato, M. R. Cozzi, M. Battiston, M. Jandrot-Perrus, M. Mongiat, P. Marchese, T. J. Kunicki, Z. M. Ruggeri, and L. De Marco Distinct spatio-temporal Ca2+ signaling elicited by integrin {alpha}2{beta}1 and glycoprotein VI under flow Blood, September 24, 2009; 114(13): 2793 - 2801. [Abstract] [Full Text] [PDF] X. Yan, B. D. Johnson, and R. J. Orentas Induction of a VLA-2 (CD49b)-Expressing Effector T Cell Population by a Cell-Based Neuroblastoma Vaccine Expressing CD137L J. Immunol., October 1, 2008; 181(7): 4621 - 4631. [Abstract] [Full Text] [PDF] R. J. Westrick, M. E. Winn, and D. T. Eitzman Murine Models of Vascular Thrombosis Arterioscler Thromb Vasc Biol, October 1, 2007; 27(10): 2079 - 2093. [Abstract] [Full Text] [PDF] U. J.H. Sachs and B. Nieswandt In Vivo Thrombus Formation in Murine Models Circ. Res., April 13, 2007; 100(7): 979 - 991. [Abstract] [Full Text] [PDF] Y. Cheli and T. J. Kunicki hnRNP L regulates differences in expression of mouse integrin {alpha}2beta1 Blood, June 1, 2006; 107(11): 4391 - 4398. [Abstract] [Full Text] [PDF] K. L. Sarratt, H. Chen, M. M. Zutter, S. A. Santoro, D. A. Hammer, and M. L. Kahn GPVI and {alpha}2{beta}1 play independent critical roles during platelet adhesion and aggregate formation to collagen under flow Blood, August 15, 2005; 106(4): 1268 - 1277. [Abstract] [Full Text] [PDF] T. J. Kunicki, A. B. Federici, D. R. Salomon, J. A. Koziol, S. R. Head, T. S. Mondala, J. D. Chismar, L. Baronciani, M. T. Canciani, and I. R. Peake An association of candidate gene haplotypes and bleeding severity in von Willebrand disease (VWD) type 1 pedigrees Blood, October 15, 2004; 104(8): 2359 - 2367. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020