Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 May 2004, Vol. 103, No. 9, pp. 3396-3402.
Prepublished online as a Blood First Edition Paper on January 22, 2004; DOI 10.1182/blood-2003-10-3721.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2003-10-3721v1
103/9/3396    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, T.-T.
Right arrow Articles by Bray, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, T.-T.
Right arrow Articles by Bray, P. F.
Related Collections
Right arrow Hemostasis, Thrombosis, and Vascular Biology
Right arrow Cell Adhesion and Motility
Right arrow Signal Transduction
Right arrowRelated Article in Blood Online
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY

Genetic variation responsible for mouse strain differences in integrin {alpha}2 expression is associated with altered platelet responses to collagen

Tong-Tong Li, Susana Larrucea, Shiloe Souza, Suzanne M. Leal, José A. López, Edward M. Rubin, Bernhard Nieswandt, and Paul F. Bray

From the Departments of Medicine and Molecular and Human Genetics, Baylor College of Medicine, Houston, TX; the Lawrence Berkeley Laboratories, Berkeley, CA; and the Department of Vascular Biology, Rudolf Virchow Center for Experimental Biomedicine Versbacher, Würzburg, Germany.

As mouse models have become commonplace for studying hemostasis and thrombosis, we considered whether the mouse system had utility for assessing genetic alterations in platelet receptors. Platelets from 5 mouse strains (C57BL/6 [C57], FVB/N [FVB], BALB/c, C3H/He, and 129Sv) showed only minor differences in the expression of integrin {alpha}IIb, integrin {beta}3, glycoprotein (GP) Ib{alpha}, or GPVI across strains. However, FVB platelets expressed approximately 50% the level of integrin {alpha}2 as platelets from other strains (P < .0001). We bred FVB mice with C57 and assessed {alpha}2 expression in FVB/C57xFVB/C57 (F2) offspring. Linkage analysis demonstrated the gene responsible for {alpha}2 levels is tightly linked to the D13mit260 marker (log odds [lod] score 6.7) near the {alpha}2 gene. FVB platelets showed reduced aggregation and a longer lag phase to collagen. FVB and C57 platelets aggregated similarly to collagen-related peptide, but FVB platelets showed a reduction in rhodocytin-induced Syk and PLC{gamma}2 tyrosine phosphorylation. Thus, FVB platelets express half the level of {alpha}2 as other mouse strains, a trait linked to the {alpha}2 gene and seemingly responsible for reduced platelet aggregation to collagen. These strain differences serve as a useful model for the 2-fold difference in human platelet {alpha}2{beta}1 expression and demonstrate that {alpha}2{beta}1 participates in signaling during platelet activation. (Blood. 2004;103:3396-3402)


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article in Blood Online:

Of men; why not mice?
Thomas J. Kunicki
Blood 2004 103: 3251-3252. [Full Text] [PDF]



This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
R. J. Westrick, M. E. Winn, and D. T. Eitzman
Murine Models of Vascular Thrombosis
Arterioscler. Thromb. Vasc. Biol., October 1, 2007; 27(10): 2079 - 2093.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
U. J.H. Sachs and B. Nieswandt
In Vivo Thrombus Formation in Murine Models
Circ. Res., April 13, 2007; 100(7): 979 - 991.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Cheli and T. J. Kunicki
hnRNP L regulates differences in expression of mouse integrin {alpha}2beta1
Blood, June 1, 2006; 107(11): 4391 - 4398.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. L. Sarratt, H. Chen, M. M. Zutter, S. A. Santoro, D. A. Hammer, and M. L. Kahn
GPVI and {alpha}2{beta}1 play independent critical roles during platelet adhesion and aggregate formation to collagen under flow
Blood, August 15, 2005; 106(4): 1268 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. J. Kunicki, A. B. Federici, D. R. Salomon, J. A. Koziol, S. R. Head, T. S. Mondala, J. D. Chismar, L. Baronciani, M. T. Canciani, and I. R. Peake
An association of candidate gene haplotypes and bleeding severity in von Willebrand disease (VWD) type 1 pedigrees
Blood, October 15, 2004; 104(8): 2359 - 2367.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020