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Blood, 1 July 2004, Vol. 104, No. 1, pp. 200-206. Prepublished online as a Blood First Edition Paper on March 11, 2004; DOI 10.1182/blood-2003-11-4072. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-11-4072v1 104/1/200    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, B. Articles by Cao, X. Search for Related Content PubMed PubMed Citation Articles by Wang, B. Articles by Cao, X. Related Collections Immunobiology Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Identification of an HLA-A*0201–restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein Baomei Wang, Huabiao Chen, Xiaodong Jiang, Minghui Zhang, Tao Wan, Nan Li, Xiangyang Zhou, Yanfeng Wu, Feng Yang, Yizhi Yu, Xiaoning Wang, Ruifu Yang, and Xuetao Cao From the Institute of Immunology, Second Military Medical University, Shanghai, People's Republic of China; the Institute of Immunology, Zhejiang University, Hangzhou, People's Republic of China; the Institute of Molecular Immunology, First Military Medical University, Guangzhou, People's Republic of China; and the Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing, People's Republic of China. A novel coronavirus, severe acute respiratory syndrome (SARS)–associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein–derived peptides was tested for their binding affinity to HLA-A*0201 molecules. Peptides with high affinity for HLA-A*0201 were then assessed for their capacity to elicit specific immune responses mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A2.1/Kb transgenic mice, and in vitro, from peripheral blood lymphocytes (PBLs) sourced from healthy HLA-A2.1+ donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced peptide-specific CTLs both in vivo (transgenic mice) and in vitro (human PBLs), which specifically released interferon- (IFN-) upon stimulation with SSp-1–pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1–specific CTLs also lysed major histocompatibility complex (MHC)–matched tumor cell lines engineered to express S proteins. HLA-A*0201–SSp-1 tetramer staining revealed the presence of significant populations of SSp-1–specific CTLs in SSp-1–induced CD8+ T cells. We propose that the newly identified epitope SSp-1 will help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherapeutic approaches for SARS. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Yang, E. James, M. Roti, L. Huston, J. A. Gebe, and W. W. Kwok Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals Int. Immunol., January 1, 2009; 21(1): 63 - 71. [Abstract] [Full Text] [PDF] L. Yang, H. Peng, Z. Zhu, G. Li, Z. Huang, Z. Zhao, R. A. Koup, R. T. Bailer, and C. Wu Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen J. Gen. Virol., October 1, 2007; 88(10): 2740 - 2748. [Abstract] [Full Text] [PDF] Y. Yu, H. Jin, Z. Chen, Q. L Yu, Y. J Ma, X. L Sun, and B. Wang Children's vaccines do not induce cross reactivity against SARS-CoV J. Clin. Pathol., February 1, 2007; 60(2): 208 - 211. [Abstract] [Full Text] [PDF] M. Zhou, D. Xu, X. Li, H. Li, M. Shan, J. Tang, M. Wang, F.-S. Wang, X. Zhu, H. Tao, et al. Screening and Identification of Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific CTL Epitopes J. Immunol., August 15, 2006; 177(4): 2138 - 2145. [Abstract] [Full Text] [PDF] K.-M. Lip, S. Shen, X. Yang, C.-T. Keng, A. Zhang, H.-L. J. Oh, Z.-H. Li, L.-A. Hwang, C.-F. Chou, B. C. Fielding, et al. Monoclonal Antibodies Targeting the HR2 Domain and the Region Immediately Upstream of the HR2 of the S Protein Neutralize In Vitro Infection of Severe Acute Respiratory Syndrome Coronavirus J. Virol., January 15, 2006; 80(2): 941 - 950. [Abstract] [Full Text] [PDF] S. Dai, T. Wan, B. Wang, X. Zhou, F. Xiu, T. Chen, Y. Wu, and X. Cao More Efficient Induction of HLA-A*0201-Restricted and Carcinoembryonic Antigen (CEA)-Specific CTL Response by Immunization with Exosomes Prepared from Heat-Stressed CEA-Positive Tumor Cells Clin. Cancer Res., October 15, 2005; 11(20): 7554 - 7563. [Abstract] [Full Text] [PDF] H. Chen, J. Hou, X. Jiang, S. Ma, M. Meng, B. Wang, M. Zhang, M. Zhang, X. Tang, F. Zhang, et al. Response of Memory CD8+ T Cells to Severe Acute Respiratory Syndrome (SARS) Coronavirus in Recovered SARS Patients and Healthy Individuals J. Immunol., July 1, 2005; 175(1): 591 - 598. [Abstract] [Full Text] [PDF] Y. Wu, T. Wan, X. Zhou, B. Wang, F. Yang, N. Li, G. Chen, S. Dai, S. Liu, M. Zhang, et al. Hsp70-Like Protein 1 Fusion Protein Enhances Induction of Carcinoembryonic Antigen-Specific CD8+ CTL Response by Dendritic Cell Vaccine Cancer Res., June 1, 2005; 65(11): 4947 - 4954. [Abstract] [Full Text] [PDF] A. Chen, L. Wang, J. Zhang, L. Zou, Z. Jia, W. Zhou, Y. Wan, and Y. Wu H-2 Kd-Restricted Hepatitis B Virus-Derived Epitope Whose Specific CD8+ T Lymphocytes Can Produce Gamma Interferon without Cytotoxicity J. Virol., May 1, 2005; 79(9): 5568 - 5576. [Abstract] [Full Text] [PDF]

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