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Blood, 1 July 2004, Vol. 104, No. 1, pp. 290-294. Prepublished online as a Blood First Edition Paper on March 9, 2004; DOI 10.1182/blood-2003-03-0688. This Article Full Text Full Text (PDF) Supplemental Video All Versions of this Article: 2003-03-0688v1 104/1/290    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shi, D. Articles by Torok-Storb, B. Search for Related Content PubMed PubMed Citation Articles by Shi, D. Articles by Torok-Storb, B. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Myogenic fusion of human bone marrow stromal cells, but not hematopoietic cells Daqing Shi, Hans Reinecke, Charles E. Murry, and Beverly Torok-Storb From the Fred Hutchinson Cancer Research Center, Division of Clinical Research, and the Department of Pathology, University of Washington, Seattle, WA. Following marrow transplantation in both patients and animals, cells containing donor nuclei have been detected in a variety of nonhematopoietic tissue. Whether this phenomenon represents transdifferentiation of marrow-derived cells, infiltration of blood cells, or cell fusion is still controversial. In muscle, where cell fusion occurs during normal myogenesis, fusion of marrow-derived cells with resident myotubes is a likely explanation. We tested 8 subpopulations of human bone marrow for their ability to fuse with mouse C2C12 myoblast cells. Relatively high fusion efficiency was observed with marrow stromal cells whereas hematopoietic cells, including populations enriched for stem cells, progenitor cells, and monocytes were refractory to fusion. Mouse myotubes containing human nuclei also contained transcripts for human muscle–specific genes. Injection in vivo of human stromal cells expressing green fluorescent protein (GFP) into the regenerating tibialis anterior muscle of nonobese diabetic–severe combined immunodeficient (NOD/SCID) 2m–/– mice resulted in regenerating mouse muscle fibers expressing GFP. These data suggest that marrow-derived cells contribute to myogenesis through fusion and that stromal cells are better fusion partners than hematopoietic cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Murakami, X. Chen, K. Hase, A. Sakamoto, C. Nishigaki, and H. Ohno Splenic CD19 CD35+B220+ cells function as an inducer of follicular dendritic cell network formation Blood, August 15, 2007; 110(4): 1215 - 1224. [Abstract] [Full Text] [PDF] S. Li, E. Kimura, R. Ng, B. M. Fall, L. Meuse, M. Reyes, J. A. Faulkner, and J. S. Chamberlain A highly functional mini-dystrophin/GFP fusion gene for cell and gene therapy studies of Duchenne muscular dystrophy Hum. Mol. Genet., May 15, 2006; 15(10): 1610 - 1622. [Abstract] [Full Text] [PDF] B. B. Ayach, M. Yoshimitsu, F. Dawood, M. Sun, S. Arab, M. Chen, K. Higuchi, C. Siatskas, P. Lee, H. Lim, et al. Stem cell factor receptor induces progenitor and natural killer cell-mediated cardiac survival and repair after myocardial infarction PNAS, February 14, 2006; 103(7): 2304 - 2309. [Abstract] [Full Text] [PDF] M. A.F.V. Goncalves, A. A.F. de Vries, M. Holkers, M. J.M. van de Watering, I. van der Velde, G. P. van Nierop, D. Valerio, and S. Knaan-Shanzer Human mesenchymal stem cells ectopically expressing full-length dystrophin can complement Duchenne muscular dystrophy myotubes by cell fusion Hum. Mol. Genet., January 15, 2006; 15(2): 213 - 221. [Abstract] [Full Text] [PDF] L. Wang, Y. Liu, Z. Kalajzic, X. Jiang, and D. W. Rowe Heterogeneity of engrafted bone-lining cells after systemic and local transplantation Blood, November 15, 2005; 106(10): 3650 - 3657. [Abstract] [Full Text] [PDF] A. Sacco, R. Doyonnas, M. A. LaBarge, M. M. Hammer, P. Kraft, and H. M. Blau IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors J. Cell Biol., November 7, 2005; 171(3): 483 - 492. [Abstract] [Full Text] [PDF] S. Fazel, L. Chen, R. D. Weisel, D. Angoulvant, C. Seneviratne, A. Fazel, P. Cheung, J. Lam, P. W.M. Fedak, T. M. Yau, et al. Cell transplantation preserves cardiac function after infarction by infarct stabilization: Augmentation by stem cell factor J. Thorac. Cardiovasc. Surg., November 1, 2005; 130(5): 1310 - 1310. [Abstract] [Full Text] [PDF] H. K. Haider and M. Ashraf Bone marrow stem cell transplantation for cardiac repair Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2557 - H2567. [Abstract] [Full Text] [PDF]

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