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Blood, 15 November 2004, Vol. 104, No. 10, pp. 3106-3116. Prepublished online as a Blood First Edition Paper on July 20, 2004; DOI 10.1182/blood-2004-04-1333. This Article Full Text Full Text (PDF) Supplemental Table and Figures All Versions of this Article: 2004-04-1333v1 104/10/3106    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Valverde-Garduno, V. Articles by Vyas, P. Search for Related Content PubMed PubMed Citation Articles by Valverde-Garduno, V. Articles by Vyas, P. Related Collections Hematopoiesis and Stem Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification Veronica Valverde-Garduno, Boris Guyot, Eduardo Anguita, Isla Hamlett, Catherine Porcher, and Paresh Vyas From the Department of Haematology and the Medical Research Council (MRC) Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. Cis-element identification is a prerequisite to understand transcriptional regulation of gene loci. From analysis of a limited number of conserved gene loci, sequence comparison has proved a robust and efficient way to locate cis-elements. Human and mouse GATA1 genes encode a critical hematopoietic transcription factor conserved in expression and function. Proper control of GATA1 transcription is critical in regulating myeloid lineage specification and maturation. Here, we compared sequence and systematically mapped position of DNase I hypersensitive sites, acetylation status of histone H3/H4, and in vivo binding of transcription factors over approximately 120 kilobases flanking the human GATA1 gene and the corresponding region in mice. Despite lying in approximately 10 megabase (Mb) conserved syntenic segment, the chromatin structures of the 2 homologous loci are strikingly different. The 2 previously unidentified hematopoietic cis-elements, one in each species, are not conserved in position and sequence and have enhancer activity in erythroid cells. In vivo, they both bind the transcription factors GATA1, SCL, LMO2, and Ldb1. More broadly, there are both species- and regulatory element–specific patterns of transcription factor binding. These findings suggest that some cis-elements regulating human and mouse GATA1 genes differ. More generally, mouse human sequence comparison may fail to identify all cis-elements. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. R. Migliaccio, F. Martelli, M. Verrucci, M. Sanchez, M. Valeri, G. Migliaccio, A. M. Vannucchi, M. Zingariello, A. Di Baldassarre, B. Ghinassi, et al. Gata1 expression driven by the alternative HS2 enhancer in the spleen rescues the hematopoietic failure induced by the hypomorphic Gata1low mutation Blood, September 3, 2009; 114(10): 2107 - 2120. [Abstract] [Full Text] [PDF] N. K. Wilson, D. Miranda-Saavedra, S. Kinston, N. Bonadies, S. D. Foster, F. Calero-Nieto, M. A. Dawson, I. J. Donaldson, S. Dumon, J. Frampton, et al. The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development Blood, May 28, 2009; 113(22): 5456 - 5465. [Abstract] [Full Text] [PDF] T. Tripic, W. Deng, Y. Cheng, Y. Zhang, C. R. Vakoc, G. D. Gregory, R. C. Hardison, and G. A. Blobel SCL and associated proteins distinguish active from repressive GATA transcription factor complexes Blood, March 5, 2009; 113(10): 2191 - 2201. [Abstract] [Full Text] [PDF] M. Suzuki, T. Moriguchi, K. Ohneda, and M. Yamamoto Differential Contribution of the Gata1 Gene Hematopoietic Enhancer to Erythroid Differentiation Mol. Cell. Biol., March 1, 2009; 29(5): 1163 - 1175. [Abstract] [Full Text] [PDF] Y. Cheng, D. C. King, L. C. Dore, X. Zhang, Y. Zhou, Y. Zhang, C. Dorman, D. Abebe, S. A. Kumar, F. Chiaromonte, et al. Transcriptional enhancement by GATA1-occupied DNA segments is strongly associated with evolutionary constraint on the binding site motif Genome Res., December 1, 2008; 18(12): 1896 - 1905. [Abstract] [Full Text] [PDF] I. Hamlett, J. Draper, J. Strouboulis, F. Iborra, C. Porcher, and P. 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[Abstract] [Full Text] [PDF] B. Guyot, K. Murai, Y. Fujiwara, V. Valverde-Garduno, M. Hammett, S. Wells, N. Dear, S. H. Orkin, C. Porcher, and P. Vyas Characterization of a Megakaryocyte-specific Enhancer of the Key Hemopoietic Transcription Factor GATA1 J. Biol. Chem., May 12, 2006; 281(19): 13733 - 13742. [Abstract] [Full Text] [PDF] R. M. Johnson, T. Prychitko, D. Gumucio, D. E. Wildman, M. Uddin, and M. Goodman Phylogenetic comparisons suggest that distance from the locus control region guides developmental expression of primate beta-type globin genes PNAS, February 28, 2006; 103(9): 3186 - 3191. [Abstract] [Full Text] [PDF] A. H. Schuh, A. J. Tipping, A. J. Clark, I. Hamlett, B. Guyot, F. J. Iborra, P. Rodriguez, J. Strouboulis, T. Enver, P. Vyas, et al. ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis Mol. Cell. Biol., December 1, 2005; 25(23): 10235 - 10250. [Abstract] [Full Text] [PDF] S. Rodriguez-Jato, R. D. 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