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 Blood, 15 November 2004, Vol. 104, No. 10, pp. 3148-3152.
Prepublished online as a Blood First Edition Paper on July 22, 2004; DOI 10.1182/blood-2004-03-0835.

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HEMATOPOIESIS

G6PD is indispensable for erythropoiesis after the embryonic-adult hemoglobin switch

Francesca Paglialunga, Annalisa Fico, Ingram Iaccarino, Rosario Notaro, Lucio Luzzatto, Giuseppe Martini, and Stefania Filosa

From the Istituto di Genetica e Biofisica "Adriano Buzzati Traverso" CNR, Napoli; IST, Istituto Nazionale per la Ricerca sul Cancro, Genova; and Università degli Studi di Genova, Italy.

Glucose 6-phosphate dehydrogenase (G6PD) (EC 1.1.1.42) is an essential enzyme for the rapid production of NADPH, as required on exposure to oxidative stress. Mouse embryonic stem (ES) cells can produce all embryonic and fetal/adult cell types. By studying the in vitro differentiation of embryoid bodies produced from G6pd{Delta} ES cells that are totally unable to produce G6PD protein, we found that these cells are able to differentiate into mesodermal cells, cardiomyocytes, hepatocytes, and primitive erythroid cells. However, we show here that, after the hemoglobin switch has taken place, definitive erythrocytes die by apoptosis. This apoptotic death is delayed by reducing agents and by a caspase inhibitor, but it is prevented only by the restoration of G6PD activity. Thus, G6PD proves indispensable for definitive erythropoiesis.


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Related Article in Blood Online:

G6PD activity is essential for definite erythropoiesis
Josef T. Prchal
Blood 2004 104: 2997. [Full Text] [PDF]





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