Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 15 November 2004, Vol. 104, No. 10, pp. 3161-3168.
Prepublished online as a Blood First Edition Paper on July 22, 2004; DOI 10.1182/blood-2004-03-0893.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-03-0893v1
104/10/3161    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kanaji, T.
Right arrow Articles by Ware, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kanaji, T.
Right arrow Articles by Ware, J.
Related Collections
Right arrow Cell Cycle
Right arrow Cell Adhesion and Motility
Right arrow Signal Transduction
Right arrow Hematopoiesis and Stem Cells
Right arrowRelated Article in Blood Online
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

HEMATOPOIESIS

Megakaryocyte proliferation and ploidy regulated by the cytoplasmic tail of glycoprotein Ib{alpha}

Taisuke Kanaji, Susan Russell, Janet Cunningham, Kenji Izuhara, Joan E. B. Fox, and Jerry Ware

From the Department of Molecular and Experimental Medicine, Division of Experimental Hemostasis and Thrombosis, Roon Research Center for Arteriosclerosis and Thrombosis, La Jolla, CA; the Department of Molecular Cardiology, Joseph J. Jacobs Center for Thrombosis and Vascular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, OH; and the Department of Biomolecular Sciences, Saga Medical School, Japan.

We have investigated the ability of glycoprotein (GP) Ib{alpha}, a megakaryocytic gene product, to sequester the signal transduction protein 14-3-3{xi} and to influence megakaryocytopoiesis. Using a Gp1ba–/– mouse colony, we compared the rescued phenotypes produced by a wild-type human GP Ib{alpha} allele or a similar allele containing a 6-residue cytoplasmic tail truncation that abrogates binding to 14-3-3{xi}. The observed phenotypes illustrate an involvement for GP Ib{alpha} in thrombopoietin-mediated events of megakaryocyte proliferation, polyploidization, and the expression of apoptotic markers in maturing megakaryocytes. We developed a hypothesis for the involvement of a GP Ib{alpha}/14-3-3{xi}/PI-3 kinase complex in regulating thrombopoietin-mediated responses. An observed increase in thrombopoietin-mediated Akt phosphorylation in the truncated variant supported the hypothesis and led to the development of a model in which the GP Ib{alpha} cytoplasmic tail sequestered signaling proteins during megakaryocytopoiesis and, as such, became a critical regulator in the temporal sequence of events that led to normal megakaryocyte maturation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article in Blood Online:

A tail with a leading role in megakaryocytes: the glycoprotein Ib
Katya Ravid
Blood 2004 104: 3004-3005. [Full Text] [PDF]



This article has been cited by other articles:


Home page
 BloodHome page
F.-T. Mu, R. K. Andrews, J. F. Arthur, A. D. Munday, S. L. Cranmer, S. P. Jackson, F. C. Stomski, A. F. Lopez, and M. C. Berndt
A functional 14-3-3{zeta}-independent association of PI3-kinase with glycoprotein Ib{alpha}, the major ligand-binding subunit of the platelet glycoprotein Ib-IX-V complex
Blood, May 1, 2008; 111(9): 4580 - 4587.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Iannacone, G. Sitia, M. Isogawa, J. K. Whitmire, P. Marchese, F. V. Chisari, Z. M. Ruggeri, and L. G. Guidotti
Platelets prevent IFN-{alpha}/ -induced lethal hemorrhage promoting CTL-dependent clearance of lymphocytic choriomeningitis virus
PNAS, January 15, 2008; 105(2): 629 - 634.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Jain, M. Zuka, J. Liu, S. Russell, J. Dent, J. A. Guerrero, J. Forsyth, B. Maruszak, T. K. Gartner, B. Felding-Habermann, et al.
Platelet glycoprotein Ib{alpha} supports experimental lung metastasis
PNAS, May 22, 2007; 104(21): 9024 - 9028.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Li, J. Lu, and E. V. Prochownik
c-Myc-mediated genomic instability proceeds via a megakaryocytic endomitosis pathway involving Gp1b{alpha}
PNAS, February 27, 2007; 104(9): 3490 - 3495.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Guerriero, I. Parolini, U. Testa, P. Samoggia, E. Petrucci, M. Sargiacomo, C. Chelucci, M. Gabbianelli, and C. Peschle
Inhibition of TPO-induced MEK or mTOR activity induces opposite effects on the ploidy of human differentiating megakaryocytes
J. Cell Sci., February 15, 2006; 119(4): 744 - 752.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
Sponsor: Genentech BioOncology and and Biogen Idec
Blood Online is supported in part by
Genentech BioOncology and Biogen Idec
  Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020