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Blood, 15 November 2004, Vol. 104, No. 10, pp. 3228-3230.
Prepublished online as a Blood First Edition Paper on July 22, 2004; DOI 10.1182/blood-2004-04-1428.
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HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Brief report
Visualization of lymphatic vessels through NF- B activity
Marcia R. Saban,
Sylvie Mémet,
David G. Jackson,
John Ash,
Aurelio A. Roig,
Alain Israël, and
Ricardo Saban
From the Department of Physiology, University Oklahoma Health Sciences Center, Oklahoma City; Unité de Biologie Moléculaire de l'Expression Génique, URA CNRS 2582, Institut Pasteur, Paris, France; Medical Research Council (MRC) Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford, United Kingdom; and Department of Ophthalmology, University of Oklahoma Health Sciences Center, Dean A. McGee Eye Institute, Oklahoma City.
The molecular biology of lymphatics is only rudimentary owing to the long-standing absence of specific markers, and scanty is the information regarding bladder lymphatic vessels. By using mice with a reporter gene for nuclear factor  B (NF-B) activity (B-lacZ) in combination with immunohistochemical staining with a specific lymphatic marker (LYVE-1), we show, for the first time, that NF-B is constitutively active in lymphatic endothelium in the urinary bladder, uterus, intestine, heart, and airways. Tie2-lacZ mice confirmed that the structures observed in B-lacZ mice were not blood vessels. In addition, acute instillation of lipopolysaccharide (LPS) or tumor necrosis factor  (TNF-) into the B-lacZ mouse bladder revealed the capacity of this transgenic in reporting inducible NF-B activity. Our findings demonstrate an overriding constitutive NF-B activity in the lymphatic system. They also provide a working model for detecting lymphatic vessels and evoke testable hypotheses regarding the role of lymphatic vessels in health and disease.
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[Abstract]
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