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Blood, 15 December 2004, Vol. 104, No. 13, pp. 3918-3926. Prepublished online as a Blood First Edition Paper on August 26, 2004; DOI 10.1182/blood-2004-05-1845. This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 2004-05-1845v1 104/13/3918    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Haddad, R. Articles by Canque, B. Search for Related Content PubMed PubMed Citation Articles by Haddad, R. Articles by Canque, B. Related Collections Hematopoiesis and Stem Cells Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood Rima Haddad, Philippe Guardiola, Brigitte Izac, Christelle Thibault, Jerry Radich, Anne-Lise Delezoide, Claude Baillou, François M. Lemoine, Jean Claude Gluckman, Françoise Pflumio, and Bruno Canque From the EMI-0013 Institut National de la Santé et de la Recherche Médicale-Université Paris 7, and Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Institut Universitaire d'Hématologie, Hôpital Saint-Louis, Paris, France; Experimental Pathology, Fred Hutchinson Cancer Research Center, Seattle WA; Département d'Hématologie, Institut Cochin, Institut National de la Santé et de la Recherche Médicale (INSERM) U567, Paris; Institut de Génétique et Biologie Moléculaire et Cellulaire, Strasbourg, France; Service de Biologie du Développement, Hôpital Robert Debré AP-HP, Paris, France; and the Centre National de la Recherche Scientifique (CNRS) Unite Mixte de Recherche (UMR) 7087, Hôpital Pitié-Salpêtrière AP-HP, Paris, France. The early stages of human lymphopoiesis are poorly characterized. Here, we compared the lymphoid potential of a novel umbilical cord blood CD34+CD45RAhiCD7+ hematopoietic progenitor cell (HPC) population with that of CD34+CD45RAhiLin-CD10+ HPCs, previously proposed as candidate common lymphoid progenitors. Limiting-dilution and clonal analysis, fetal thymic organ cultures, and culture onto Notch ligand Delta-like-1-expressing OP9 cells, showed that although CD34+CD45RAhiCD7+ HPCs could generate cells of the 3 lymphoid lineages, their potential was skewed toward the T/natural killer (T/NK) lineages. In contrast, CD34+CD45RAhiLin-CD10+ HPCs predominantly exhibited a B-cell potential. Gene expression profiling with DNA microarrays confirmed that CD34+CD45RAhiCD7+ HPCs selectively expressed T-lymphoid and NK lineage-committed genes while retaining expression of genes affiliated to the granulomonocytic lineage, whereas CD34+CD45RAhiLin-CD10+ HPCs displayed a typical pro-B-cell transcription profile and essentially lacked genes unrelated to the B lineage. In addition, both populations could be generated in vitro from CD34+CD45RAintCD7- and CD34+CD45RAhiLin- HPCs with mixed lymphomyeloid potential, from which they emerged independently with different growth/differentiation factor requirements. These findings indicate that CD34+CD45RAhiCD7+ and CD34+CD45RAhiLin-CD10+ HPCs correspond to multipotent early lymphoid progenitors polarized toward either the T/NK or B lineage, respectively. (Blood. 2004;104: 3918-3926) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Strehl, K. Nebral, M. Konig, J. Harbott, H. Strobl, R. Ratei, S. Struski, B. Bielorai, M. Lessard, M. Zimmermann, et al. ETV6-NCOA2: A Novel Fusion Gene in Acute Leukemia Associated with Coexpression of T-Lymphoid and Myeloid Markers and Frequent NOTCH1 Mutations Clin. Cancer Res., February 15, 2008; 14(4): 977 - 983. [Abstract] [Full Text] [PDF] R. Haddad, F. Pflumio, I. Vigon, G. Visentin, C. 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