SEARCH:   Advanced

Blood, 15 December 2004, Vol. 104, No. 13, pp. 4020-4028. Prepublished online as a Blood First Edition Paper on August 10, 2004; DOI 10.1182/blood-2004-03-0885. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-03-0885v1 104/13/4020    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bella, S. D. Articles by Berardi, A. C. Search for Related Content PubMed PubMed Citation Articles by Bella, S. D. Articles by Berardi, A. C. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Are interleukin-16 and thrombopoietin new tools for the in vitro generation of dendritic cells? Silvia Della Bella, Stefania Nicola, Inna Timofeeva, Maria Luisa Villa, Armando Santoro, and Anna C. Berardi From the Dipartimento di Scienze e Tecnologie Biomediche, Laboratorio di Immunologia, Università di Milano, Milan, Italy; Istituto Clinica Humanitas, Laboratorio di Ematologia/Oncologia, Rozzano, Milan, Italy; Ospedale Pediatrico Bambino Gesù, Laboratorio di Ricerche "Cellule Staminali," Rome, Italy. The effects of interleukin 16 (IL-16) on dendritic cell (DC) generation from human CD34+ progenitor cells are not known. Here, we show that IL-16 added to a basal cocktail comprised of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-4, Flt-3 ligand (Flt3L), and tumor necrosis factor (TNF-) does induce the CD34+ hematopoietic cells to proliferate in vitro and to differentiate into phenotypically and functionally mature DCs. IL-16 exerts this function more efficiently than stem cell factor (SCF) as a control, thrombopoietin (TPO), or IL-16 plus TPO. Moreover, we show that the combination of IL-16 plus TPO induces the generation of tolerogenic DCs, able to induce an anergic state in T cells that persists when T cells are rechallenged with immunogenic DCs. An altered pattern of cytokine production, a reduced expression of the C-type lectin DC-SIGN, and an increased surface expression of the inhibitory molecules immunoglobulin-like transcript 2 (ILT-2), ILT-3, and ILT-4 may all contribute to confer the tolerogenic properties of these DCs. Generation of tolerogenic DCs may aid the exploration of new therapeutic strategies to promote tolerance to autoantigens and prevent disease development. (Blood. 2004;104:4020-4028) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Rutella, S. Danese, and G. Leone Tolerogenic dendritic cells: cytokine modulation comes of age Blood, September 1, 2006; 108(5): 1435 - 1440. [Abstract] [Full Text] [PDF] V. Moos, D. Kunkel, T. Marth, G. E. Feurle, B. LaScola, R. Ignatius, M. Zeitz, and T. Schneider Reduced Peripheral and Mucosal Tropheryma whipplei-Specific Th1 Response in Patients with Whipple's Disease J. Immunol., August 1, 2006; 177(3): 2015 - 2022. [Abstract] [Full Text] [PDF] B. Desnues, D. Raoult, and J.-L. Mege IL-16 Is Critical for Tropheryma whipplei Replication in Whipple's Disease J. Immunol., October 1, 2005; 175(7): 4575 - 4582. [Abstract] [Full Text] [PDF] G. Vlad, R. Cortesini, and N. Suciu-Foca License to Heal: Bidirectional Interaction of Antigen-Specific Regulatory T Cells and Tolerogenic APC J. Immunol., May 15, 2005; 174(10): 5907 - 5914. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020