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Blood, 15 December 2004, Vol. 104, No. 13, pp. 4104-4112. Prepublished online as a Blood First Edition Paper on August 19, 2004; DOI 10.1182/blood-2004-05-1986. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: 2004-05-1986v1 104/13/4104    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gauguet, J.-M. Articles by von Andrian, U. H. Search for Related Content PubMed PubMed Citation Articles by Gauguet, J.-M. Articles by von Andrian, U. H. Related Collections Cell Adhesion and Motility Hemostasis, Thrombosis, and Vascular Biology Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Core 2 branching 1,6-N-acetylglucosaminyltransferase and high endothelial cell N-acetylglucosamine-6-sulfotransferase exert differential control over B- and T-lymphocyte homing to peripheral lymph nodes Jean-Marc Gauguet, Steven D. Rosen, Jamey D. Marth, and Ulrich H. von Andrian From the CBR Institute for Biomedical Research and the Department of Pathology, Harvard Medical School, Boston, MA; the Department of Anatomy, Program in Immunology, Cardiovascular Research Institute, University of California at San Francisco; and the Howard Hughes Medical Institute and the Department of Cellular and Molecular Medicine, University of California, San Diego. Blood-borne lymphocyte trafficking to peripheral lymph nodes (PLNs) depends on the successful initiation of rolling interactions mediated by L-selectin binding to sialomucin ligands in high endothelial venules (HEVs). Biochemical analysis of purified L-selectin ligands has identified posttranslational modifications mediated by Core2GlcNAcT-I and high endothelial cell GlcNAc-6-sulfotransferase (HECGlcNAc6ST). Consequently, lymphocyte migration to PLNs of C2GlcNAcT-I-/- and HEC-GlcNAc6ST-/- mice was reduced; however, B-cell homing was more severely compromised than T-cell migration. Accordingly, intravital microscopy (IVM) of PLN HEVs revealed a defect in B-cell tethering and increased rolling velocity (Vroll) in C2GlcNAcT-I-/- mice that was more pronounced than it was for T cells. By contrast, B- and T-cell tethering was normal in HEC-GlcNAc6ST-/- HEVs, but Vroll was accelerated, especially for B cells. The increased sensitivity of B cells to glycan deficiencies was caused by lower expression levels of L-selectin; L-selectin+/- T cells expressing L-selectin levels equivalent to those of B cells exhibited intravascular behavior similar to that of B cells. These results demonstrate distinct functions for C2GlcNAcT-I and HEC-GlcNAc6ST in the differential elaboration of HEV glycoproteins that set a threshold for the amount of L-selectin needed for lymphocyte homing. (Blood. 2004;104:4104-4112) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Tenno, K. Ohtsubo, F. K. Hagen, D. Ditto, A. Zarbock, P. Schaerli, U. H. von Andrian, K. Ley, D. Le, L. A. Tabak, et al. Initiation of Protein O Glycosylation by the Polypeptide GalNAcT-1 in Vascular Biology and Humoral Immunity Mol. Cell. Biol., December 15, 2007; 27(24): 8783 - 8796. [Abstract] [Full Text] [PDF] J. D Canete, B. Santiago, T. Cantaert, R. Sanmarti, A. Palacin, R. Celis, E. Graell, B. Gil-Torregrosa, D. Baeten, and J. L Pablos Ectopic lymphoid neogenesis in psoriatic arthritis Ann Rheum Dis, June 1, 2007; 66(6): 720 - 726. [Abstract] [Full Text] [PDF] L. M. Yu, D. X. Chen, Q. X. Zhou, N. Fang, and Z. L. Liu Effects of Histamine on Immunophenotype and Notch Signaling in Human HL-60 Leukemia Cells. Experimental Biology and Medicine, November 1, 2006; 231(10): 1633 - 1637. [Abstract] [Full Text] [PDF] C. Halin, M. L. Scimone, R. Bonasio, J.-M. Gauguet, T. R. Mempel, E. Quackenbush, R. L. Proia, S. Mandala, and U. H. von Andrian The S1P-analog FTY720 differentially modulates T-cell homing via HEV: T-cell-expressed S1P1 amplifies integrin activation in peripheral lymph nodes but not in Peyer patches Blood, August 15, 2005; 106(4): 1314 - 1322. [Abstract] [Full Text] [PDF]

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