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Blood, 1 August 2004, Vol. 104, No. 3, pp. 675-686. Prepublished online as a Blood First Edition Paper on April 13, 2004; DOI 10.1182/blood-2003-10-3423. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-10-3423v1 104/3/675    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, W. Articles by Ho, A. D. Search for Related Content PubMed PubMed Citation Articles by Wagner, W. Articles by Ho, A. D. Related Collections Hematopoiesis and Stem Cells Gene Expression Genomics Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis Wolfgang Wagner, Alexandra Ansorge, Ute Wirkner, Volker Eckstein, Christian Schwager, Jonathon Blake, Katrin Miesala, Jan Selig, Rainer Saffrich, Wilhelm Ansorge, and Anthony D. Ho From the Department of Medicine V, University of Heidelberg, Heidelberg, Germany; and Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany. The molecular mechanisms that regulate asymmetric divisions of hematopoietic progenitor cells (HPCs) are not yet understood. The slow-dividing fraction (SDF) of HPCs is associated with primitive function and self-renewal, whereas the fast-dividing fraction (FDF) predominantly proceeds to differentiation. CD34+/CD38– cells of human umbilical cord blood were separated into the SDF and FDF. Genomewide gene expression analysis of these populations was determined using the newly developed Human Transcriptome Microarray containing 51 145 cDNA clones of the Unigene Set-RZPD3. In addition, gene expression profiles of CD34+/CD38– cells were compared with those of CD34+/CD38+ cells. Among the genes showing the highest expression levels in the SDF were the following: CD133, ERG, cyclin G2, MDR1, osteopontin, CLQR1, IFI16, JAK3, FZD6, and HOXA9, a pattern compatible with their primitive function and self-renewal capacity. Furthermore, morphologic differences between the SDF and FDF were determined. Cells in the SDF have more membrane protrusions and CD133 is located on these lamellipodia. The majority of cells in the SDF are rhodamine-123dull. These results provide molecular evidence that the SDF is associated with primitive function and serves as basis for a detailed understanding of asymmetric division of stem cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Stem cells and the Heisenberg uncertainty principle H. Jeffrey Lawrence Blood 2004 104: 597-598. [Full Text] [PDF] This article has been cited by other articles: F. P. G. Silva, S. M. A. Swagemakers, C. Erpelinck-Verschueren, B. J. Wouters, R. Delwel, H. Vrieling, P. van der Spek, P. J. M. Valk, and M. Giphart-Gassler Gene expression profiling of minimally differentiated acute myeloid leukemia: M0 is a distinct entity subdivided by RUNX1 mutation status Blood, October 1, 2009; 114(14): 3001 - 3007. [Abstract] [Full Text] [PDF] A. Kandilci and G. C. 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