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Blood, 15 August 2004, Vol. 104, No. 4, pp. 1025-1033. Prepublished online as a Blood First Edition Paper on May 4, 2004; DOI 10.1182/blood-2003-09-3334. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-09-3334v1 104/4/1025    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yuan, K. Articles by Lin, M. T. Search for Related Content PubMed PubMed Citation Articles by Yuan, K. Articles by Lin, M. T. Related Collections Cell Adhesion and Motility Signal Transduction Gene Expression Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Syndecan-1 up-regulated by ephrinB2/EphB4 plays dual roles in inflammatory angiogenesis Kuo Yuan, Tse-Ming Hong, Jeremy J. W. Chen, Wan Hua Tsai, and Ming T. Lin From the Periodontics Division, Department of Dentistry, National Cheng Kung University Hospital, Tainan, Taiwan; School of Pharmacy, National Taiwan University College of Medicine, Taipei, Taiwan; Institute of Biomedical Sciences and Molecular Biology, National Chung Hsing University, Taichung, Taiwan; and Department of Biochemistry and Institute of Basic Medical Sciences, Medical School, National Cheng Kung University, Tainan, Taiwan. EphrinB2 and EphB4, its cognate receptor, are important in the vascular development of the mouse embryo. Their roles in human inflammatory angiogenesis, however, are not well understood. By examining hyperinflammatory lesions, we saw that ephrinB2 was predominantly expressed in macrophage-like cells and EphB4 in small venules. Because macrophages usually transmigrate through postcapillary venules during inflammation, we wanted to explore the downstream effects of EphB4 after binding to ephrinB2. By using cDNA microarray technique and following reverse transcriptase–polymerase chain reaction (RT-PCR), we found that syntenin and syndecan-1 were up-regulated in EphB4-positive endothelial cells dose dependently and time dependently after stimulation with preclustered ephrinB2. In vitro, ephrinB2 suppressed the angiogenic effects of basic fibroblast growth factor (bFGF) on EphB4-positive endothelial cells, partially due to syndecan-1's competition with fibroblast growth factor receptor (FGFR) for bFGF. However, ephrinB2 exhibited angiogenic effects in vivo, possibly due to an inflammation-associated enzyme—heparanase. The enzymes could convert the inhibitory effect of ephrinB2 on EphB4-positive endothelial cells to an activating effect by removing poorly sulfated side chains of up-regulated syndecan-1 ectodomain. Depending on the presence of heparanases, the roles of syndecan-1 may be opposite in different physiological settings. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Cappuzzello, R. Melchionna, A. Mangoni, G. Tripodi, P. Ferrari, L. Torielli, D. Arcelli, M. Helmer-Citterich, G. Bianchi, M. C. Capogrossi, et al. Role of rat {alpha} adducin in angiogenesis: Null effect of the F316Y polymorphism Cardiovasc Res, August 1, 2007; 75(3): 608 - 617. [Abstract] [Full Text] [PDF] A. A. ROMANOVSKY, A. I. IVANOV, and S. R. PETERSEN Microsomal Prostaglandin E Synthase-1, Ephrins, and Ephrin Kinases as Suspected Therapeutic Targets in Arthritis: Exposed by "Criminal Profiling" Ann. N.Y. Acad. Sci., June 1, 2006; 1069(1): 183 - 194. [Abstract] [Full Text] [PDF] S. Mitola, M. Belleri, C. Urbinati, D. Coltrini, B. Sparatore, M. Pedrazzi, E. Melloni, and M. Presta Cutting Edge: Extracellular High Mobility Group Box-1 Protein Is a Proangiogenic Cytokine J. Immunol., January 1, 2006; 176(1): 12 - 15. [Abstract] [Full Text] [PDF] E. Tkachenko, J. M. Rhodes, and M. Simons Syndecans: New Kids on the Signaling Block Circ. Res., March 18, 2005; 96(5): 488 - 500. [Abstract] [Full Text] [PDF]

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