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Blood, 15 August 2004, Vol. 104, No. 4, pp. 1042-1047. Prepublished online as a Blood First Edition Paper on April 20, 2004; DOI 10.1182/blood-2004-01-0315. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-01-0315v1 104/4/1042    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Washington, A. V. Articles by McVicar, D. W. Search for Related Content PubMed PubMed Citation Articles by Washington, A. V. Articles by McVicar, D. W. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Phagocytes Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY A TREM family member, TLT-1, is found exclusively in the -granules of megakaryocytes and platelets A. Valance Washington, Rebecca L. Schubert, Laura Quigley, Theresa Disipio, Robert Feltz, Edward H. Cho, and Daniel W. McVicar From the Laboratory of Experimental Immunology and The Image Analysis Laboratory, Science Application International Corporation Frederick, National Cancer Institute (NCI)–Frederick, MD. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system, including neutrophils, monocyte/macrophages, and dendritic cells, via their association with DAP12. TLT-1 (TREM-like transcript-1) lies within the TREM gene cluster and contains the characteristic single V-set immunoglobulin (Ig) domain of the family, but its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif, the latter known to be used for interactions with protein tyrosine phosphatases. Here we report that TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that TLT-1 expression is up-regulated dramatically upon platelet activation. Consistent with this observation, confocal microscopy demonstrates that TLT-1 is prepackaged, along with CD62P, into both MK and platelet -granules. Differences in thrombin-induced redistribution of CD62P and TLT-1 indicate that TLT-1 is not simply cargo of -granules but may instead regulate granule construction or dispersal. Together these data show that that TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. J.A. Korporaal, C. A. Koekman, S. Verhoef, D. E. van der Wal, M. Bezemer, M. Van Eck, and J.-W. N. Akkerman Downregulation of Platelet Responsiveness Upon Contact With LDL by the Protein-Tyrosine Phosphatases SHP-1 and SHP-2 Arterioscler Thromb Vasc Biol, March 1, 2009; 29(3): 372 - 379. [Abstract] [Full Text] [PDF] C. Wong, Y. Liu, J. Yip, R. Chand, J. L. Wee, L. Oates, B. Nieswandt, A. Reheman, H. Ni, N. Beauchemin, et al. CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo Blood, February 19, 2009; 113(8): 1818 - 1828. [Abstract] [Full Text] [PDF] J. Mori, A. C. Pearce, J. C. Spalton, B. Grygielska, J. A. Eble, M. G. Tomlinson, Y. A. Senis, and S. P. Watson G6b-B Inhibits Constitutive and Agonist-induced Signaling by Glycoprotein VI and CLEC-2 J. Biol. Chem., December 19, 2008; 283(51): 35419 - 35427. [Abstract] [Full Text] [PDF] P. Haselmayer, L. Grosse-Hovest, P. von Landenberg, H. Schild, and M. P. Radsak TREM-1 ligand expression on platelets enhances neutrophil activation Blood, August 1, 2007; 110(3): 1029 - 1035. [Abstract] [Full Text] [PDF] Y. A. Senis, M. G. Tomlinson, A. Garcia, S. Dumon, V. L. Heath, J. Herbert, S. P. Cobbold, J. C. Spalton, S. Ayman, R. Antrobus, et al. A Comprehensive Proteomics and Genomics Analysis Reveals Novel Transmembrane Proteins in Human Platelets and Mouse Megakaryocytes Including G6b-B, a Novel Immunoreceptor Tyrosine-based Inhibitory Motif Protein Mol. Cell. Proteomics, March 1, 2007; 6(3): 548 - 564. [Abstract] [Full Text] [PDF] R. G. King, B. R. Herrin, and L. B. Justement Trem-Like Transcript 2 Is Expressed on Cells of the Myeloid/Granuloid and B Lymphoid Lineage and Is Up-Regulated in Response to Inflammation J. Immunol., May 15, 2006; 176(10): 6012 - 6021. [Abstract] [Full Text] [PDF] J. L. Gattis, A. V. Washington, M. M. Chisholm, L. Quigley, A. Szyk, D. W. McVicar, and J. Lubkowski The Structure of the Extracellular Domain of Triggering Receptor Expressed on Myeloid Cells Like Transcript-1 and Evidence for a Naturally Occurring Soluble Fragment J. Biol. Chem., May 12, 2006; 281(19): 13396 - 13403. [Abstract] [Full Text] [PDF] J. B. McMahon, K. R. Gustafson, and C. J. Henrich Molecular-Targeted Drug Screening at the NCI Center for Cancer Research Am. Assoc. Cancer Res. Educ. Book, April 1, 2005; 2005(1): 125 - 129. [Full Text] [PDF]

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