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Blood, 1 September 2004, Vol. 104, No. 5, pp. 1298-1305. Prepublished online as a Blood First Edition Paper on May 13, 2004; DOI 10.1182/blood-2004-01-0100. This Article Full Text Full Text (PDF) Supplemental Video All Versions of this Article: 2004-01-0100v1 104/5/1298    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Traver, D. Articles by Zon, L. I. Search for Related Content PubMed PubMed Citation Articles by Traver, D. Articles by Zon, L. I. Related Collections Hematopoiesis and Stem Cells Immunobiology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation David Traver, Alissa Winzeler, Howard M. Stern, Elizabeth A. Mayhall, David M. Langenau, Jeffrey L. Kutok, A. Thomas Look, and Leonard I. Zon From Children's Hospital Boston; the Howard Hughes Medical Institute; the Department of Pediatric Oncology, Dana-Farber Cancer Institute; and the Department of Pathology, Brigham and Women's Hospital, Boston, MA. The study of hematopoiesis has been greatly facilitated by transplantation of blood cell populations into recipient animals. Efficient engraftment of donor cells generally requires ablation of the host hematopoietic system. The zebrafish has recently emerged as a developmental and genetic system to study hematopoiesis. To enable the study of hematopoietic stem cell (HSC) biology, immune cell function, and leukemogenesis in zebrafish, we have developed hematopoietic cell transplantation (HCT) into adult recipient animals conditioned by irradiation. Dose-response experiments showed that the minimum lethal dose (MLD) of 40 Gy led to the specific ablation of hematolymphoid cells and death by 14 days after irradiation. Sublethal irradiation doses of 20 Gy predominantly ablated lymphocytes and permitted transplantation of a lethal T-cell leukemia. Finally, transplantation of hematopoietic cells carrying transgenes yielding red fluorescent erythrocytes and green fluorescent leukocytes showed that HCT is sufficient to rescue the MLD, that recipient hematolymphoid tissues were repopulated by donor-derived cells, and that donor blood cell lineages can be independently visualized in living recipients. Together, these results establish transplantation assays to test for HSC function and oncogenic transformation in zebrafish. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Y. Bertrand, A. D. Kim, S. Teng, and D. Traver CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis Development, May 15, 2008; 135(10): 1853 - 1862. [Abstract] [Full Text] [PDF] I. Kobayashi, K. Saito, T. Moritomo, K. Araki, F. Takizawa, and T. Nakanishi Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue Blood, February 1, 2008; 111(3): 1131 - 1137. [Abstract] [Full Text] [PDF] J. A. Martinez-Agosto, H. K.A. Mikkola, V. Hartenstein, and U. Banerjee The hematopoietic stem cell and its niche: a comparative view Genes & Dev., December 1, 2007; 21(23): 3044 - 3060. [Abstract] [Full Text] [PDF] C. E. Burns, D. Traver, E. Mayhall, J. L. Shepard, and L. I. Zon Hematopoietic stem cell fate is established by the Notch-Runx pathway Genes & Dev., October 1, 2005; 19(19): 2331 - 2342. [Abstract] [Full Text] [PDF]

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