SEARCH:   Advanced

Blood, 1 November 2004, Vol. 104, No. 9, pp. 2752-2760. Prepublished online as a Blood First Edition Paper on June 29, 2004; DOI 10.1182/blood-2004-04-1396. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-04-1396v1 104/9/2752    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ingram, D. A. Articles by Yoder, M. C. Search for Related Content PubMed PubMed Citation Articles by Ingram, D. A. Articles by Yoder, M. C. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood David A. Ingram, Laura E. Mead, Hiromi Tanaka, Virginia Meade, Amy Fenoglio, Kelly Mortell, Karen Pollok, Michael J. Ferkowicz, David Gilley, and Mervin C. Yoder From the Department of Pediatrics, Herman B. Wells Center for Pediatric Research, the Department of Biochemistry and Molecular Biology, and the Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis. Emerging evidence to support the use of endothelial progenitor cells (EPCs) for angiogenic therapies or as biomarkers to assess cardiovascular disease risk and progression is compelling. However, there is no uniform definition of an EPC, which makes interpretation of these studies difficult. Although hallmarks of stem and progenitor cells are their ability to proliferate and to give rise to functional progeny, EPCs are primarily defined by the expression of cell-surface antigens. Here, using adult peripheral and umbilical cord blood, we describe an approach that identifies a novel hierarchy of EPCs based on their clonogenic and proliferative potential, analogous to the hematopoietic cell system. In fact, some EPCs form replatable colonies when deposited at the single-cell level. Using this approach, we also identify a previously unrecognized population of EPCs in cord blood that can achieve at least 100 population doublings, replate into at least secondary and tertiary colonies, and retain high levels of telomerase activity. Thus, these studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferative potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood. (Blood. 2004;104:2752-2760) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Endothelial progenitor cells: reporting for duty Monica Bessler Blood 2004 104: 2616-2617. [Full Text] [PDF] This article has been cited by other articles: B. Li, A. Vincent, J. Cates, D. M. Brantley-Sieders, D. B. Polk, and P. P. Young Low Levels of Tumor Necrosis Factor {alpha} Increase Tumor Growth by Inducing an Endothelial Phenotype of Monocytes Recruited to the Tumor Site Cancer Res., January 1, 2009; 69(1): 338 - 348. [Abstract] [Full Text] [PDF] A.O. Robb, N.L. Mills, I.B.J. Smith, A. Short, O. Tura-Ceide, G.R. Barclay, A. Blomberg, H.O.D. Critchley, D.E. Newby, and F.C. Denison Influence of menstrual cycle on circulating endothelial progenitor cells Hum. Reprod., December 16, 2008; (2008) den411v1. [Abstract] [Full Text] [PDF] J. M. Melero-Martin, M. E. De Obaldia, S.-Y. Kang, Z. A. Khan, L. Yuan, P. Oettgen, and J. Bischoff Engineering Robust and Functional Vascular Networks In Vivo With Human Adult and Cord Blood-Derived Progenitor Cells Circ. Res., July 18, 2008; 103(2): 194 - 202. [Abstract] [Full Text] [PDF] A. Zeoli, P. Dentelli, A. Rosso, G. Togliatto, A. Trombetta, L. Damiano, P. F. di Celle, L. Pegoraro, F. Altruda, and M. F. Brizzi Interleukin-3 promotes expansion of hemopoietic-derived CD45+ angiogenic cells and their arterial commitment via STAT5 activation Blood, July 15, 2008; 112(2): 350 - 361. [Abstract] [Full Text] [PDF] A. Surdacki, E. Marewicz, E. Wieteska, G. Szastak, T. Rakowski, E. Wieczorek-Surdacka, D. Dudek, J. Pryjma, and J. S. Dubiel Association between endothelial progenitor cell depletion in blood and mild-to-moderate renal insufficiency in stable angina Nephrol. Dial. Transplant., July 1, 2008; 23(7): 2265 - 2273. [Abstract] [Full Text] [PDF] M. Thill, N. V. Strunnikova, M. J. Berna, N. Gordiyenko, K. Schmid, S. W. Cousins, D. J. S. Thompson, and K. G. Csaky Late Outgrowth Endothelial Progenitor Cells in Patients with Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2696 - 2708. [Abstract] [Full Text] [PDF] C Kalka and I. Baumgartner Gene and stem cell therapy in peripheral arterial occlusive disease Vascular Medicine, May 1, 2008; 13(2): 157 - 172. [Abstract] [PDF] D. A. Ingram, I. Z. Lien, L. E. Mead, M. Estes, D. N. Prater, E. Derr-Yellin, L. A. DiMeglio, and L. S. Haneline In Vitro Hyperglycemia or a Diabetic Intrauterine Environment Reduces Neonatal Endothelial Colony-Forming Cell Numbers and Function Diabetes, March 1, 2008; 57(3): 724 - 731. [Abstract] [Full Text] [PDF] J. Clark, D. F. Alvarez, M. Alexeyev, J. A. C. King, L. Huang, M. C. Yoder, and T. Stevens Regulatory role for nucleosome assembly protein-1 in the proliferative and vasculogenic phenotype of pulmonary endothelium Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L431 - L439. [Abstract] [Full Text] [PDF] D. F. Alvarez, L. Huang, J. A. King, M. K. ElZarrad, M. C. Yoder, and T. Stevens Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L419 - L430. [Abstract] [Full Text] [PDF] K. Asosingh, M. A. Aldred, A. Vasanji, J. Drazba, J. Sharp, C. Farver, S. A.A. Comhair, W. Xu, L. Licina, L. Huang, et al. Circulating Angiogenic Precursors in Idiopathic Pulmonary Arterial Hypertension Am. J. Pathol., March 1, 2008; 172(3): 615 - 627. [Abstract] [Full Text] [PDF] D. C. Rafii, B. Psaila, J. Butler, D. K. Jin, and D. Lyden Regulation of Vasculogenesis by Platelet-Mediated Recruitment of Bone Marrow-Derived Cells Arterioscler. Thromb. Vasc. Biol., February 1, 2008; 28(2): 217 - 222. [Abstract] [Full Text] [PDF] P. Au, L. M. Daheron, D. G. Duda, K. S. Cohen, J. A. Tyrrell, R. M. Lanning, D. Fukumura, D. T. Scadden, and R. K. Jain Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels Blood, February 1, 2008; 111(3): 1302 - 1305. [Abstract] [Full Text] [PDF] D. O. Traktuev, S. Merfeld-Clauss, J. Li, M. Kolonin, W. Arap, R. Pasqualini, B. H. Johnstone, and K. L. March A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks Circ. Res., January 4, 2008; 102(1): 77 - 85. [Abstract] [Full Text] [PDF] Y. Suarez, B. R. Shepherd, D. A. Rao, and J. S. Pober Alloimmunity to Human Endothelial Cells Derived from Cord Blood Progenitors J. Immunol., December 1, 2007; 179(11): 7488 - 7496. [Abstract] [Full Text] [PDF] G. Grenier, A. Scime, F. Le Grand, A. Asakura, C. Perez-Iratxeta, M. A. Andrade-Navarro, P. A. Labosky, and M. A. Rudnicki Resident Endothelial Precursors in Muscle, Adipose, and Dermis Contribute to Postnatal Vasculogenesis Stem Cells, December 1, 2007; 25(12): 3101 - 3110. [Abstract] [Full Text] [PDF] L. Cai, B. H. Johnstone, T. G. Cook, Z. Liang, D. Traktuev, K. Cornetta, D. A. Ingram, E. D. Rosen, and K. L. March Suppression of Hepatocyte Growth Factor Production Impairs the Ability of Adipose-Derived Stem Cells to Promote Ischemic Tissue Revascularization Stem Cells, December 1, 2007; 25(12): 3234 - 3243. [Abstract] [Full Text] [PDF] X. Wu, M. W. Lensch, J. Wylie-Sears, G. Q. Daley, and J. Bischoff Hemogenic Endothelial Progenitor Cells Isolated from Human Umbilical Cord Blood Stem Cells, November 1, 2007; 25(11): 2770 - 2776. [Abstract] [Full Text] [PDF] W. B. Slayton, X.-M. Li, J. Butler, S. M. Guthrie, M. L. Jorgensen, J. R. Wingard, and E. W. Scott The Role of the Donor in the Repair of the Marrow Vascular Niche Following Hematopoietic Stem Cell Transplant Stem Cells, November 1, 2007; 25(11): 2945 - 2955. [Abstract] [Full Text] [PDF] M. Ferrario, M. Massa, V. Rosti, R. Campanelli, M. Ferlini, B. Marinoni, G. M. De Ferrari, V. Meli, M. De Amici, A. Repetto, et al. Early haemoglobin-independent increase of plasma erythropoietin levels in patients with acute myocardial infarction Eur. Heart J., August 1, 2007; 28(15): 1805 - 1813. [Abstract] [Full Text] [PDF] U. M. Gehling, S. Ergun, and W. Fiedler CFU-EC: how they were originally defined Blood, August 1, 2007; 110(3): 1073 - 1073. [Full Text] [PDF] F. Timmermans, F. Van Hauwermeiren, M. De Smedt, R. Raedt, F. Plasschaert, M. L. De Buyzere, T. C. Gillebert, J. Plum, and B. Vandekerckhove Endothelial Outgrowth Cells Are Not Derived From CD133+ Cells or CD45+ Hematopoietic Precursors Arterioscler. Thromb. Vasc. Biol., July 1, 2007; 27(7): 1572 - 1579. [Abstract] [Full Text] [PDF] E. Rohde, C. Bartmann, K. Schallmoser, A. Reinisch, G. Lanzer, W. Linkesch, C. Guelly, and D. Strunk Immune Cells Mimic the Morphology of Endothelial Progenitor Colonies In Vitro Stem Cells, July 1, 2007; 25(7): 1746 - 1752. [Abstract] [Full Text] [PDF] M. Nagano, T. Yamashita, H. Hamada, K. Ohneda, K.-i. Kimura, T. Nakagawa, M. Shibuya, H. Yoshikawa, and O. Ohneda Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood Blood, July 1, 2007; 110(1): 151 - 160. [Abstract] [Full Text] [PDF] H. Yao, B. Liu, X. Wang, Y. Lan, N. Hou, X. Yang, and N. Mao Identification of High Proliferative Potential Precursors with Hemangioblastic Activity in the Mouse Aorta-Gonad- Mesonephros Region Stem Cells, June 1, 2007; 25(6): 1423 - 1430. [Abstract] [Full Text] [PDF] J. M. Melero-Martin, Z. A. Khan, A. Picard, X. Wu, S. Paruchuri, and J. Bischoff In vivo vasculogenic potential of human blood-derived endothelial progenitor cells Blood, June 1, 2007; 109(11): 4761 - 4768. [Abstract] [Full Text] [PDF] V. Balasubramaniam, C. F. Mervis, A. M. Maxey, N. E. Markham, and S. H. Abman Hyperoxia reduces bone marrow, circulating, and lung endothelial progenitor cells in the developing lung: implications for the pathogenesis of bronchopulmonary dysplasia Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1073 - L1084. [Abstract] [Full Text] [PDF] M. C. Yoder, L. E. Mead, D. Prater, T. R. Krier, K. N. Mroueh, F. Li, R. Krasich, C. J. Temm, J. T. Prchal, and D. A. Ingram Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals Blood, March 1, 2007; 109(5): 1801 - 1809. [Abstract] [Full Text] [PDF] D. G. Duda, K. S. Cohen, P. Au, D. T. Scadden, C. G. Willett, and R. K. Jain In Reply J. Clin. Oncol., February 10, 2007; 25(5): e3 - e5. [Full Text] [PDF] D. A. Ingram, T. R. Krier, L. E. Mead, C. McGuire, D. N. Prater, J. Bhavsar, M. R. Saadatzadeh, K. Bijangi-Vishehsaraei, F. Li, M. C. Yoder, et al. Clonogenic Endothelial Progenitor Cells Are Sensitive to Oxidative Stress Stem Cells, February 1, 2007; 25(2): 297 - 304. [Abstract] [Full Text] [PDF] G. C. Schatteman, M. Dunnwald, and C. Jiao Biology of bone marrow-derived endothelial cell precursors Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18. [Abstract] [Full Text] [PDF] R. M. Shepherd, B. J. Capoccia, S. M. Devine, J. DiPersio, K. M. Trinkaus, D. Ingram, and D. C. Link Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100 Blood, December 1, 2006; 108(12): 3662 - 3667. [Abstract] [Full Text] [PDF] H. M. Gilbert, A. I. Sergueva, V. R. Gordeuk, and J. T. Prchal Homocysteine Metabolism in Chuvash Polycythemia. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 4911 - 4911. [Abstract] [PDF] K. H. Wu, Y. L. Liu, B. Zhou, and Z. C. Han Cellular therapy and myocardial tissue engineering: the role of adult stem and progenitor cells Eur. J. Cardiothorac. Surg., November 1, 2006; 30(5): 770 - 781. [Abstract] [Full Text] [PDF] C. J. Boos, G. Y.H. Lip, and A. D. Blann Circulating Endothelial Cells in Cardiovascular Disease J. Am. Coll. Cardiol., October 17, 2006; 48(8): 1538 - 1547. [Abstract] [Full Text] [PDF] J. Leor and M. Marber Endothelial Progenitors: A New Tower of Babel? J. Am. Coll. Cardiol., October 17, 2006; 48(8): 1588 - 1590. [Full Text] [PDF] H. Guven, R. M. Shepherd, R. G. Bach, B. J. Capoccia, and D. C. Link The Number of Endothelial Progenitor Cell Colonies in the Blood Is Increased in Patients With Angiographically Significant Coronary Artery Disease J. Am. Coll. Cardiol., October 17, 2006; 48(8): 1579 - 1587. [Abstract] [Full Text] [PDF] F. Li, A. M. Munchhof, H. A. White, L. E. Mead, T. R. Krier, A. Fenoglio, S. Chen, X. Wu, S. Cai, F.-C. Yang, et al. Neurofibromin is a novel regulator of RAS-induced signals in primary vascular smooth muscle cells Hum. Mol. Genet., June 1, 2006; 15(11): 1921 - 1930. [Abstract] [Full Text] [PDF] A. M. Munchhof, F. Li, H. A. White, L. E. Mead, T. R. Krier, A. Fenoglio, X. Li, J. Yuan, F.-C. Yang, and D. A. Ingram Neurofibroma-associated growth factors activate a distinct signaling network to alter the function of neurofibromin-deficient endothelial cells Hum. Mol. Genet., June 1, 2006; 15(11): 1858 - 1869. [Abstract] [Full Text] [PDF]